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V9881

Sigma-Aldrich

Vitronectin from bovine plasma

lyophilized powder, BioReagent, suitable for cell culture

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Synonym(s):
Serum spreading factor
CAS Number:
MDL number:
NACRES:
NA.75

biological source

bovine plasma

Quality Level

product line

BioReagent

form

lyophilized powder

packaging

pkg of 50 μg

technique(s)

cell culture | mammalian: suitable

surface coverage

0.1 μg/cm2

solubility

water: soluble 1.0 mL, clear, colorless

UniProt accession no.

shipped in

ambient

storage temp.

2-8°C

Gene Information

bovine ... VTN(507525)

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vibrant-m

V9881

Vitronectin from bovine plasma

vibrant-m

V8379

Vitronectin from human plasma

vibrant-m

V0132

Vitronectin from rat plasma

vibrant-m

F2379

Fetuin from fetal bovine serum

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

Gene Information

bovine ... VTN(507525)

Gene Information

human ... VTN(7448)

Gene Information

rat ... Vtn(29169)

Gene Information

bovine ... FETUB(504615)

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

solubility

water: soluble 1.0 mL, clear, colorless

solubility

water: soluble 0.050 mg/mL, clear, colorless

solubility

water: soluble 1.00 mL, clear, colorless

solubility

H2O: 1 mg/mL, colorless to faint yellow or tan

Application

Vitronectin can be used with cells with integrin receptors that bind vitronectin, including platelets, endothelial cells, melanoma cells, and osteosarcoma. It is recommended as a cell culture substratum at 0.1 μg/cm2. Optimal conditions for attachment depend on cell line and application.

Biochem/physiol Actions

Vitronectin binds to glycosaminoglycans, is incorporated as an inhibitor to the membrane cytolytic attack complex of the complement system, has interaction with thrombin and antithrombin III during coagulation, and may play a role in the coagulation pathway.

Components

Vitronectin is a glycoprotein present in plasma and tissues, and is one of the major cell adhesion proteins, along with fibronectin, in plasma. The N-terminal residues of vitronectin are identical to somatomedin B and are followed by an R-G-D sequence that interacts with a specific cell-surface receptor. The central domain of vitronectin is enriched with hydrophobic residues. Closer to the C-terminus, it has an arginine rich region that allows for heparin binding activity.

Caution

The lyophilized product should be stored desiccated at 2-8°C. Under these conditions it is stable for at least 2 years. Aliquots should be stored at -20°C or -70°C, but repeated freezing and thawing should be avoided.

Preparation Note

This product is supplied as a cell culture tested lyophilized powder from buffered saline. It is recommended to use polypropylene vessels during handling, due to the strong affinity of vitronectin for hydrophilic surfaces. Reconstitution of this product can be done with 1 mL of water per vial. The stock solution should then be filter-sterilized using a 0.2μm membrane and aliquoted and frozen for long term storage.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Stephanie L Gupton et al.
The Journal of cell biology, 198(4), 657-676 (2012-08-22)
Mena is an Ena/VASP family actin regulator with roles in cell migration, chemotaxis, cell-cell adhesion, tumor cell invasion, and metastasis. Although enriched in focal adhesions, Mena has no established function within these structures. We find that Mena forms an adhesion-regulated
Yusuke Arima et al.
Acta biomaterialia, 26, 72-81 (2015-08-27)
We examined the effect of surface chemistry on adsorption of fibronectin (Fn) and vitronectin (Vn) and subsequent cell adhesion, employing self-assembled monolayers (SAMs) of alkanethiols carrying terminal methyl (CH3), hydroxyl groups (OH), carboxylic acid (COOH), and amine (NH2). More Fn
Joseph Mathew Kalappurakkal et al.
Cell, 177(7), 1738-1756 (2019-05-21)
Glycosylphosphatidylinositol-anchored proteins (GPI-APs) are a major class of lipid-anchored plasma membrane proteins. GPI-APs form nanoclusters generated by cortical acto-myosin activity. While our understanding of the physical principles governing this process is emerging, the molecular machinery and functional relevance of GPI-AP

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