Y4645

Anti-phospho-YAP1 (pTyr³⁵⁷) antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-YAP, Anti-YAP2, Anti-YAP65, Anti-Yes-associated protein 1, 65kDa
• MDL number: MFCD04118707
• UNSPSC Code: 12352203
• NACRES: NA.44

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~65 kDa
• species reactivity: human
• packaging: antibody small pack of 25 μL
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• concentration: ~1.5 mg/mL
• technique(s): indirect immunofluorescence: 5-10 μg/mL using HEK-293 cells co-transfected with human YAP1 and c-Abl; western blot: 0.5-1 μg/mL using HEK-293T cells co-transfected with human YAP1 and human c-Abl
• UniProt accession no.: P46937
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: phosphorylation (pTyr357)
• Gene Information: chicken ... YAP1(396171) ; human ... YAP1(10413) ; mouse ... Yap1(22601)

Description

General description

Yes-associated protein 1 (YAP1) is a 65 kDa and a modular adapter protein with multiple protein interaction domains that was originally identified based on its interaction with Src-family tyrosine kinase c-Yes. In addition to a SH3-binding motif, YAP1 contains a proline-rich amino terminus, a WW domain, a coiled-coil and a PDZ-binding motif at the extreme C-terminus. YAP1 binds to p73 through its WW domain and the PPPY motif of p73. This interaction is required for the ability of YAP1 to co-activate p73-responsive genes.

Specificity

Anti-phospho-YAP1 pTyr³⁵⁷ antibody is specific for human phospho-YAP1 pTyr³⁵⁷. The product may react with chicken and mouse phospho-YAP1 pTyr³⁵⁷. In immunoblotting, staining of the phospho-YAP1 (pTyr357) band is specifically inhibited by the immunizing peptide; but no inhibition is observed with the unmodified peptide.

Immunogen

synthetic peptide corresponding to amino acids 351-362 (pTyr³⁵⁷) located in the C-terminal region of human YAP1. The sequence is identical in chicken YAP1 and highly conserved in mouse YAP1 (single amino acid substitution).

Application

Anti-phospho-YAP1 (pTyr357) antibody produced in rabbit has been used in western blotting and immunofluorescence.

Biochem/physiol Actions

Inhibition of Akt potentiates the nuclear re-localization of YAP1 to induce apoptosis by p73.

YAP1 is a WW domain containing protein that is known to associate with a wide range of transcription factors and functions as a transcriptional co-activator. It may also regulate p73 gene function in response to DNA damage. Alterations in YAP1 have been associated with tumor development
Anti-phospho-YAP1 pTyr³⁵⁷ antibody is specific for human phospho-YAP1 pTyr³⁵⁷. The product may react with chicken and mouse phospho-YAP1 pTyr³⁵⁷. In immunoblotting, staining of the phospho-YAP1 (pTyr357) band is specifically inhibited by the immunizing peptide; but no inhibition is observed with the unmodified peptide.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable