General description
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency".
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ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 29D1 is a ZooMAb® Mouse recombinant monoclonal antibody that specifically detect WHSC1/NSD2.
Immunogen
GST-tagged recombinant protein corresponding to human WHSC1/NSD2
Application
Quality Control Testing
Evaluated by Western Blotting in HeLa cell nuclear extract.
Western Blotting Analysis (WB): A 1:10,000 dilution of this antibody detected WHSC1/NSD2 in HeLa cell nuclear extract.
Tested Applications
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected WHSC1/NSD2 in NIH3T3 cell lysates.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected WHSC1/NSD2 in human testis tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected WHSC1/NSD2 in HeLa and NIH3T3 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Target description
Histone-lysine N-methyltransferase NSD2 (UniProt: O96028; also known as EC:2.1.1.357, Multiple myeloma SET domain-containing protein, MMSET, Nuclear SET domain-containing protein 2, Protein trithorax-5, Wolf-Hirschhorn syndrome candidate, WHSC1) is encoded by the NSD2 (also known as KIAA1090, MMSET, TRX5, WHSC1) gene (Gene ID: 7468) in human. WHSC1 is a histone methyltransferase that methylates histone H3 at lysine 36 and is involved in the regulation of gene transcription during various biological processes. It is widely expressed, and its higher expression is observed in thymus and testis. It is reported to specifically dimethylate nucleosomal histone H3 at lysine 36 (H3K36me2) and can monomethylate nucleosomal histone H3 at lysine 36 (H3K36me) in vitro. At the euchromatic regions in embryonic stem cells (ESC) it is shown to trimethylate lysine 36 (H3K36me3). In ESC, it associates with developmental transcription factors such as SALL1 via PHD-type zinc fingers and represses inappropriate gene transcription mediated by histone deacetylation. WHSC1 is reported to play an essential role in adipogenesis, by regulating expression of genes involved in pre-adipocyte differentiation. A chromosomal aberration involving NSD2 is shown to cause multiple myeloma. Overexpression of WHSC1 is reported in a variety of cancer cells and its knockdown decreases proliferation in cancer cells at least in part by loss of H3K36me2 levels. Seven different isoforms of WHSC1 have been described that are produced by alternative splicing. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Tanaka, H., et al. (2020). Aging Cell. 19(7); e13173; Zhuang, L., et al. (2018). Nat. Commun. 9(1); 1796; Kuo, AJ., et al. (2011). Mol. Cell. 44(4); 609-620; Morango, J., et al. (2008). Blood. 111(6); 3145-3254).
Physical form
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Reconstitution
300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.