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ZRB1111

Sigma-Aldrich

Anti-eIF3h Antibody, clone 2B15 ZooMAb® Rabbit Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

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biological source

rabbit

Quality Level

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

2B15, monoclonal
recombinant monoclonal

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt ~39.93 kDa
observed mol wt ~40 kDa

species reactivity

rat, human, mouse

species reactivity (predicted by homology)

bovine, monkey

packaging

antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

isotype

IgG

UniProt accession no.

greener alternative category

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

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species reactivity

rat, human, mouse

species reactivity

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species reactivity

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species reactivity

human

conjugate

unconjugated

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conjugate

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conjugate

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clone

2B15, monoclonal, recombinant monoclonal

clone

2I15, recombinant monoclonal

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2B19, monoclonal, recombinant monoclonal

clone

2N15, recombinant monoclonal

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

UniProt accession no.

O15372

UniProt accession no.

P04062

UniProt accession no.

P35318

UniProt accession no.

P10747

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General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 2B15 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Eukaryotic translation initiation factor 3 subunit H (elF3h). It targets an epitope within 21 amino acids from the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 21 amino acids from the C-terminal region of human Eukaryotic translation initiation factor 3 subunit H (elF3h).

Application

Quality Control Testing

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected eIF3h in HEK293 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected eIF3h in A431, PC12, and NIH3T3 cell lysates.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected eIF3h in human pancreas tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected eIF3h in A431 and HepG2 cells.

Affinity Binding Assay: A representative lot of this antibody bound eIF3H peptide with a KD of 1.1 x 10-6 in an Affinity Binding Assay,

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
This rabbit recombinant monoclonal Anti-eIF3h, clone 2B15 ZooMAb® Rabbit Monoclonal, Cat. No. ZRB1111 is used in Western Blotting, Affinity Binding Assay, Immunocytochemistry and Immunohistochemistry (Paraffin), for the detection of eIF3h.

Target description

Eukaryotic translation initiation factor 3 subunit H (UniProt: O15372; also known as eIF3h, Eukaryotic translation initiation factor 3 subunit 3, eIF-3-gamma, eIF3 p40 subunit) is encoded by the EIF3H (also known as EIF3S3) gene (Gene ID: 8667) in human. elF3h is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex that is required for several steps in the initiation of protein synthesis. The elF3 complex is composed of 13 different subunits that are divided into three modules. elF3h belongs to module B that also contains elF3f and elf3M. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex is reported to specifically target and initiate translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Physical form

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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