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ZRB1199

Sigma-Aldrich

Anti-Zfp64 Antibody, clone 3B22 ZooMAb® Rabbit Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

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biological source

rabbit

Quality Level

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

3B22, monoclonal
recombinant monoclonal

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt ~72.22 kDa
observed mol wt ~80 kDa

species reactivity

human

species reactivity (predicted by homology)

monkey

packaging

antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

affinity binding assay: suitable
immunocytochemistry: suitable
western blot: suitable

isotype

IgG

UniProt accession no.

greener alternative category

shipped in

ambient

Storage temp.

2-8°C

target post-translational modification

unmodified

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 3B22 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Zinc finger protein 64 (Zfp64). It targets an epitope within 21 amino acids from the N-terminal half.

Immunogen

KLH-conjugated linear peptide corresponding to 21 amino acids from the N-terminal half of human Zinc finger protein 64 (Zfp64).

Application

Quality Control Testing

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Zfp64 in Jurkat cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Zfp64 in A549 cell lysate.

Affinity Binding Assay: A representative lot of this antibody bound Zfp64 peptides with a KD of 1.1 x 10-8 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Zfp64 in Jurkat cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Zfp64, clone 3B22 ZooMAb®, Cat. No. ZRB1199, is a rabbit recombinant monoclonal antibody that specifically targets Zfp64 and is tested for use in Affinity Binding Assay, Immunocytochemistry, and Western Blotting.

Target description

Zinc finger protein 64 (UniProt: Q9NPA5; also known as Zfp-64, Zinc finger protein 338) is encoded by the ZFP64 (also known as ZNF338) gene (Gene ID: 55734) in human. ZNF64 is a member of the krueppel C2H2 type zinc-finger protein family. It is a transcriptional regulatory protein with 13 zinc finger regions. ZFP64 has been identified as an essential transcription factor in MLL-rearranged leukemia where it maintains MLL expression via binding to the MLL promoter, which is the most enriched location of ZFP64. ZFP64 expression is reported to be under the control of Runx2, and it is upregulated by direct transactivation of its promoter. It is shown to associate with Notch 1 and suppress the myogenic differentiation of C2C12 cells and promotes their osteoblastic differentiation. ZFP64 is shown to promote Toll-like receptor-triggered proinflammatory and type I interferon production in macrophages by enhancing the activation of p65 subunit of NF-kB. ZFP64 can bind to the promoter of the TNF- , IL-6, and IFN-β genes in macrophages following TLR ligation. Upon stimulation of cells with lipopolysaccharide (LPS), ZFP64 associates with p65 subunit of NF-kB. Six different isoforms of ZFP64 have been described that are produced by alternative splicing. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Lu, B., et al. (2018). Cancer Cell. 34(6): 970-981; Wang, C., et al. (2013). J. Biol. Chem. 288(34); 24600-24608; Sakamoto, K., et al. (2008). J. Cell Sci. 121(10); 1613-1623).

Physical form

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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