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ZRB1742

Sigma-Aldrich

Anti-NOVA2 Antibody, clone 2J16 ZooMAb® Rabbit Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

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biological source

rabbit

Quality Level

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

2J16, recombinant monoclonal

description

recombinant, expressed in HEK 293 cells

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 49.1 kDa
observed mol wt ~52 kDa

purified by

using Protein A

species reactivity

rat, mouse, human

species reactivity (predicted by homology)

porcine, feline, bovine, canine, monkey

packaging

antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

isotype

IgG

epitope sequence

N-terminal half

Protein ID accession no.

UniProt accession no.

greener alternative category

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... NOVA2(4858)

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Gene Information

human ... NOVA2(4858)

Gene Information

-

Gene Information

-

Gene Information

-

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

clone

2J16, recombinant monoclonal

clone

2L16, recombinant monoclonal

clone

2H5, recombinant monoclonal

clone

1J16, recombinant monoclonal

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

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General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 2J16 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects RNA-binding protein NOVA2. It targets an epitope within 20 amino acids from the N-terminal half.

Immunogen

KLH-conjugated linear peptide corresponding to 20 amino acids from the N-terminal half of human RNA-binding protein NOVA2.

Application

Quality Control Testing

Evaluated by Western Blotting with recombinant Human NOVA2.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected recombinant Human NOVA2 protein.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected NOVA2 in lysates from E16 Rat brain, E16 Mouse brain E16, and Human fetal brain tissue..

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected NOVA2 in Neuro-2a cells.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected NOVA2 in human cerebral cortex tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound NOVA2 with a KD of 2.4 x 10-6 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

RNA-binding protein Nova-2 (UniProt: Q9UNW9; also known as Astrocytic NOVA1-like RNA-binding protein, Neuro-oncological ventral antigen 2) is encoded by the NOVA2 (also known as ANOVA, NOVA3) gene (Gene ID: 4858) in human. The neuron specific RNA-binding proteins NOVA1 and NOVA2 are highly homologous alternative splicing regulators. Both NOVA1 and NOVA2 have three K homology (KH) domains. They are described as autoantigens in the human autoimmune neurological disease known as paraneoplastic opsoclonus-myoclonus ataxia (POMA) that is characterized by loss of motor control in limbs and eyes. NOVA proteins regulate alternative splicing in neurons by binding pre-mRNA in a sequence-specific manner to activate exon inclusion or exclusion. NOVA2 binds RNA with high affinity and with sequence specificity that differs from NOVA1. Its expression is restricted o astrocytes. In close association with NOVA 1, it performs unique biological functions in different brain areas and cell types. NOVA2 binding to an exonic 5′-YCAY-3′ cluster changes the protein complexes assembled on pre-mRNA, blocking U1 snRNP binding and exon inclusion, whereas binding to an intronic 5′-YCAY-3′ cluster enhances spliceosome assembly and exon inclusion. It is reported to regulate alternative splicing events of several axon guidance related genes during cortical development. It controls RNA splicing in both inhibitory and excitatory neurons. This ZooMAbZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Saito, Y., et al. (2016). eLife. 5; e14371; Yang, YYL., et al. (1998). Proc. Natl. Acad. Sci. USA. 95(22); 13254-13259).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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