MilliporeSigma
All Photos(8)

Documents

ZRB1847

Sigma-Aldrich

Anti-MEK1 Antibody, clone 1F18 ZooMAb® Rabbit Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

Sign Into View Organizational & Contract Pricing

biological source

rabbit

Quality Level

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

1F18, recombinant monoclonal

description

1F18 Clone

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 43.44 kDa
observed mol wt ~43 kDa

purified by

using Protein A

species reactivity

monkey, human

species reactivity (predicted by homology)

rat, mouse, porcine, bovine

packaging

antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

affinity binding assay: suitable
immunocytochemistry: suitable
western blot: suitable

isotype

IgG

epitope sequence

C-terminal

Protein ID accession no.

UniProt accession no.

greener alternative category

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... MEK1(5604)

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
ZRB1793ZRB1270ZRB1337
antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

Gene Information

human ... MEK1(5604)

Gene Information

human ... MEN1(4221)

Gene Information

-

Gene Information

-

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

species reactivity

monkey, human

species reactivity

human, mouse, rat

species reactivity

rat

species reactivity

human

clone

1F18, recombinant monoclonal

clone

1D2, recombinant monoclonal

clone

1F11, recombinant monoclonal

clone

1F16, recombinant monoclonal

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1F18 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects MEK1. It targets an epitope within 17 amino acids from the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal region of Human MEK1.

Application

Quality Control Testing

Evaluated by Western Blotting in COS-7 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected MEK1 in COS-7 cell lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected MEK1 in HEK293 and U87MG cell lysates.

Affinity Binding Assay: A representative lot of this antibody bound MEK1 peptide with a KD of 3.3 x 10-7 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MEK1 in HEK293 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

Dual specificity mitogen-activated protein kinase kinase 1 (UniProt: Q02750; also known as EC:2.7.12.2, MAP kinase kinase 1, MAPKK 1, MKK1, ERK activator kinase 1, MAPK/ERK kinase 1, MEK 1) is encoded by the MEK1 (also known as MAP2K1, PRKMK1) gene (Gene ID: 5604) in human. MEK1 is a dual specificity protein kinase that acts as an essential component of the MAP kinase signal transduction pathway. It is widely expressed, but very low levels are detected in the brain tissue. Activation of MEK1 involves binding of extracellular ligands such as growth factors and cytokines to their membrane receptors that activates RAS and then RAF. Once activated, GTP-bound RAS can drive the formation of high-activity homodimers or heterodimers of the RAF protein kinases (ARAF, BRAF or CRAF) that can directly phosphorylate and activate MEK1 and MEK2. Their activation occurs through phosphorylation of Ser 218 and Ser 222 residues. MEK1/2 in turn can activate ERK1 and ERK2 by phosphorylating them at conserved threonine and tyrosine residues in the Thr-Glu-Tyr motif in their activation loop. MEK1 is reported to be the target of negative feed-back regulation by its substrate kinases ERK2, which can phosphorylate MEK1 on Thr 292 and facilitate dephosphorylation of the activating residues Ser 218 and Ser 222. Although MEK1/2 mutations are relatively rare in cancer, expression of constitutively active forms of their mutants are reported to be sufficient to induce oncogenic transformation of normal cells. This ZooMAbZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Caunt, CJ., et al. (2015). Nat. Rev. Cancer. 15(10); 577-592; Wu, P-K., and Park, J-I. (2015). Semin. Oncol. 42(6); 849-862).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service