All Photos(19)




Anti-Sin3A Antibody, clone 1C4 ZooMAb® Rabbit Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

Sign Into View Organizational & Contract Pricing

Paired amphipathic helix protein Sin3a;Histone deacetylase complex subunit Sin3a;Transcriptional corepressor Sin3a

biological source


Quality Level


expressed in HEK 293 cells



antibody form

purified antibody

antibody product type

primary antibodies


1C4, recombinant monoclonal


1C4 Clone

product line

ZooMAb® learn more



mol wt

calculated mol wt 145.18 kDa
observed mol wt ~160 kDa

purified by

using Protein A

species reactivity

human, mouse

species reactivity (predicted by homology)

monkey, porcine, rat


antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation


affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable



epitope sequence


Protein ID accession no.

UniProt accession no.

greener alternative category

shipped in


storage temp.


target post-translational modification


Gene Information

human ... SIN3A(25942)

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.


Clone 1C4 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Sin3A. It targets an epitope within 23 amino acids from the C-terminal region.


KLH-conjugated linear peptide corresponding to 23 amino acids from the C-terminal region of human Sin3A.


Quality Control Testing

Evaluated by Western Blotting in K562 cell nuclear extract.

Western Blotting Analysis (WB): A 1:10,000 dilution of this antibody detected Sin3A in K562 cell nuclear extract.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Sin3A in HeLa cell nuclear Extract.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Sin3A in A431, HeLa, HepG2, and NIH 3T3 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Sin3A in human testis tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound Sin3A peptide with a KD of 2.8 x 10-6 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Paired amphipathic helix protein Sin3a (UniProt: Q96ST3; also known as Histone deacetylase complex subunit Sin3a, Transcriptional corepressor Sin3a) is encoded by the SIN3A gene (Gene ID: 25942) in human. Sin3a is a transcription repressor that interacts with MXI1 to repress Myc responsive genes and antagonizes Myc oncogenic activities. It also interacts with MXD1-MAX heterodimers to repress transcription by tethering SIN3A to DNA. Sin3a also serves as an important mediator of this STAT3-targeted transcriptional repression. It directly interacts with STAT3 and promotes its deacetylation. Silencing of SIN3A gene results in a prolonged nuclear retention of activated STAT3 and enhances its recruitment to the SOCS3 promoter, concomitant with histone hyperacetylation and enhanced STAT3-dependent transcription. It is also involved in the control of the circadian rhythms via transcriptional repression of circadian target genes, such as PER1. It is expressed in the developing brain, with highest levels of expression detected in the ventricular zone of various cortical regions. It is required for cortical neuron differentiation and callosal axon elongation. Mutation in SIN3A gene have been linked to Witteveen-Kolk syndrome (WITKOS), a disease characterized by global developmental delay, mild to severe intellectual disability, and facial dysmorphism. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Icardi, L., et al. (2012). Proc. Natl. Acad. Sci. USA. 109(30); 12058-12063).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.


300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids




Not applicable


Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).


Product Number
Pack Size/Quantity

Additional examples:





enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service