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ZRB2141

Sigma-Aldrich

Anti-OAS1 Antibody, clone 1N2 ZooMAb® Rabbit Monoclonal

enhanced validation

recombinant, expressed in HEK 293 cells

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Synonym(s):
2′-5′-oligoadenylate synthase 1;EC:2.7.7.84;(2-5′)oligo(A) synthase 1;2-5A synthase 1;E18/E16;p46/p42 OAS

biological source

rabbit

Quality Level

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

1N2, recombinant monoclonal

description

1N2 Clone

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 46.03 kDa
observed mol wt ~52 kDa

purified by

using Protein A

species reactivity

human

packaging

antibody small pack of 25 μL

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

ELISA: suitable
affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG

epitope sequence

N-terminal half

Protein ID accession no.

UniProt accession no.

greener alternative category

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... OAS1(4938)

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clone

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biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

species reactivity

human

species reactivity

mouse, rat, human

species reactivity

human

species reactivity

human, monkey

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

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General description

ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1N2 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects 2′-5′-oligoadenylate synthase 1 (OAS1). It targets an epitope within 17 amino acids from the N-terminal half.

Immunogen

KLH conjugated linear peptide corresponding to 17 amino acids from the N-terminal half of human 2′-5′-oligoadenylate synthase 1 (OAS1).

application

Quality Control Testing

Evaluated by Western Blotting in lysate from HeLa cells treated with IFN- .

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected OAS1 in lysate from HeLa cells treated with IFN- .

Tested Applications

Enzyme Immunoassay (ELISA) Analysis: Various dilutions from a representative lot detected OAS1 in ELISA applicaiton.

Affinity Binding Assay: A representative lot of this antibody bound OAS1 peptide with a KD of 1.4 x 10-6 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected OAS1 in Human small intestine tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected OAS1 in HeLa cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

2′-5′-oligoadenylate synthase 1 (UniProt: P00973; also known as EC:2.7.7.84, (2-5′)oligo(A) synthase 1, 2-5A synthase 1, E18/E16, p46/p42 OAS) is encoded by the OAS1 (also known as OIAS) gene (Gene ID: 4938) in human. OAS is an interferon-induced, dsRNA-activated antiviral enzyme which plays a critical role in cellular innate antiviral response. It is frequently detected in nasal epithelium of healthy individuals and displays antiviral activity. It is involved in the synthesis of higher oligomers of 2′-5′-oligoadenylates (2-5A) from ATP which then bind to the inactive monomeric form of ribonuclease L (RNase L) leading to its dimerization and subsequent activation. Activation of RNase L then leads to degradation of cellular as well as viral RNA, resulting in the inhibition of protein synthesis and termination of viral replication. By activating RNase L, it potently inhibits severe acute SARS-CoV-2. Its C-terminal prenylation (isoform p46) is shown to be essential to initiate a SARS-CoV-2 block. The prenylated form is targeted to perinuclear structures rich in viral dsRNA, whereas the non-prenylated form (isoform p42) is diffusely localized and is unable to initiate a detectable block to SARS-CoV-2 replication. OAS1 also has antiviral effects that are independent of RNase L. OAS1 also plays a role in other cellular processes, including apoptosis, cell growth, differentiation, and gene regulation. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Wickenhagen, A. et al. (2021). Science. 374(6567); eabj3624).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

0.3 mg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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