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Anti-IL-10 Antibody, clone 1O10 ZooMAb® Rabbit Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

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Interleukin-10;Cytokine synthesis inhibitory factor;CSIF

biological source


Quality Level


expressed in HEK 293 cells



antibody form

purified antibody

antibody product type

primary antibodies


1O10, recombinant monoclonal


1O10 Clone

product line

ZooMAb® learn more



mol wt

calculated mol wt 20.52 kDa
observed mol wt ~15 kDa

purified by

using Protein A

species reactivity



antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation


affinity binding assay: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable



epitope sequence


Protein ID accession no.

UniProt accession no.

greener alternative category

shipped in


storage temp.


target post-translational modification


Gene Information

human ... IL10(3586)

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This Item
Gene Information

human ... IL10(3586)

Gene Information

human ... IL18(3606)

Gene Information

human ... MRGPRX2(117194)

Gene Information

human ... CTLA4(1493)

biological source


biological source


biological source


biological source











1O10, recombinant monoclonal


1N23, recombinant monoclonal


1O10, recombinant monoclonal


1D10, recombinant monoclonal

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

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General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.


Clone 1O10 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects IL-10.


Full-length mature (without the signal peptide) recombinant human IL-10.


Quality Control Testing

Evaluated by Western Blotting with recombinant Human IL-10.

Western Blotting Analysis (WB): A 1:10,000 dilution of this antibody detected full-length, mature, recombinant Human IL-10 (without the signal peptide).

Tested Applications

Affinity Binding Assay: A representative lot of this antibody bound recombinant Human IL-10 with a KD of 1.0 x 10-6 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis (IHC(P)): A 1:100 dilution from a representative lot detected IL-10 in human tonsil tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution o from a representative lot detected IL-10 in Jurkat cells.

Flow Cytometry Analysis: 1 µg from a representative lot detected IL-10 in one million Human peripheral blood mononuclear cells (PBMC) treated with phorbol 12-myristate 13-acetate( PMA; 500 ng/mL), Ionomycin (500 ng/mL) and Brefeldin A (100 ng/mL) for 4- 6 hours.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Interleukin-10 (UniProt: P22301; also known as IL-10, Cytokine synthesis inhibitory factor, CSIF) is encoded by the IL10 gene (Gene ID: 3586) in human. IL-10 is a homodimeric secreted anti-inflammatory cytokine produced by a variety of cells, including T-cells, macrophages, and mast cells.  It is a major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions.  It is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off to produce the mature form. IL-10 acts on many cells of the immune system to limit excessive tissue disruption caused by inflammation. During infection, it inhibits the activity of Th1 cells, NK cells, and macrophages, all of which are required for optimal pathogen clearance. It inhibits the release of pro-inflammatory cytokines.  It ameliorates the excessive Th1 and CD8+ T cell responses that are responsible for much of the immunopathology associated with infections. It is shown to control the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling. IL-10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB, which leads to JAK1 and STAT2-mediated phosphorylation of STAT3 that in turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Couper, KN., et al. (2008). J. Immunol. 180(9); 5771-5777; Yoon, SI., et al. (2006). J. Biol. Chem. 281(46); 35088-35096).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.


0.3 mg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids




Not applicable


Not applicable

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