ZRB2658

Anti-KDM2B/FBXL10 Antibody, clone 2F12 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): CXXC-type zinc finger protein 2, F-box/LRR-repeat protein 10, Jumonji domain-containing EMSY-interactor methyltransferase motif protein (Protein JEMMA)
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 2F12, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 153 kDa; observed mol wt ~140 kDa
• purified by: using Protein A
• species reactivity: human, rat
• species reactivity (predicted by homology): mouse, monkey, goat, canine, feline, bovine
• packaging: antibody small pack of 25
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: Internal
• Protein ID accession no.: NP_115979
• UniProt accession no.: Q8NHM5
• greener alternative category: Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... KDM2B(84678)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 2F12 is a ZooMAb^® rabbit recombinant monoclonal antibody that detects Lysine-specific demethylase 2B (KDM2B/FBXL10). It targets an epitope within 19 amino acids from the internal part of the protein.

Immunogen

KLH-conjugated linear peptide corresponding to 19 amino acids from the internal part of human KDM2B/FBXL10.

Application

Quality Control Testing

Evaluated by Western Blotting in C6 nuclear extract.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected KDM2B/FBXL10 in C6 nuclear extract.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected KDM2B/FBXL10 in HeLa Nuclear Extract.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected KDM2B/FBXL10 in human cerebral cortex and skin tissue sections.

Affinity Binding Assay Analysis: The binding affinity of this antibody to KDM2B/FBXL10 peptide was at least one thousand-fold (1,000X) higher than that obtained with the non-specific control peptide.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected KDM2B/FBXL10 in A-431, HeLa and HepG2 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

Lysine-specific demethylase 2B (UniProt: Q8NHM5; also known as EC:1.14.11.27, JmjC domain-containing histone demethylation protein 1B) is encoded by the KDM2B (also known as CXXC2, Fbl10, FBXL10, JHDM1B, PCCX2) gene (Gene ID: 84678) in human. Lysine-specific demethylase 2B (KDM2B/FBXL10) is a protein that plays a role in the regulation of gene expression through its demethylase activity. KDM2B/FBXL10 is a member of the Jumonji C (JmjC) domain-containing demethylase family. It consists of several functional domains, including JmjC domain responsible for histone demethylation, CXXC-type zinc finger domains that bind to DNA, and a PHD finger domain involved in protein-protein interactions. KDM2B/FBXL10 functions as a histone demethylase, targeting specific lysine residues on histone proteins. It is involved in regulating key biological processes such as cell cycle progression, development, and differentiation. Its expression is dynamically regulated during development, suggesting its importance in embryogenesis and tissue homeostasis. Aberrant expression or mutations in KDM2B gene have been associated with different types of cancer, including breast, lung, and prostate cancer. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable