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SPB®-5 Capillary GC Column

L × I.D. 30 m × 0.25 mm, df 1.00 μm

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fused silica

Quality Level


meets requirements for USP G27 and G36


-60-320 °C temperature (isothermal or programmed)

Beta value



1.00 μm


gas chromatography (GC): suitable

L × I.D.

30 m × 0.25 mm

matrix active group

Bonded; poly(5% diphenyl/95% dimethyl siloxane) phase

column type

capillary non-polar

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General description

Capillary GC column is also known as open tubular column. The carrier gas flows through the central aperture and is unrestricted throughout the length of the column.
Application: This non-polar general purpose column provides primarily a boiling point elution order with a slight increase in selectivity, especially for aromatic compounds.
USP Code: This column meets USP G27 and G36 requirements.
  • Bonded
  • Poly(5% diphenyl/95% dimethyl siloxane)
Temp. Limits:
  • ≤0.32 mm I.D., <2 μm: -60 °C to 320 °C (isothermal or programmed)
  • ≤0.32 mm I.D., ≥2 μm: -60 °C to 300 °C (isothermal or programmed)
  • ≥0.53 mm I.D., <2 μm: -60 °C to 300 °C (isothermal) or 320 °C (programmed)
  • ≥0.53 mm I.D., ≥2 μm: -60 °C to 260 °C (isothermal) or 280 °C (programmed)


SPB®-5 Capillary GC Column was used for speciation of the nitrogen compounds (quantitatively), which are polar and somewhat basic in nature, with chemiluminescence detection. It may also be used as stationary phase for determination of nivalenol and deoxynivalenol in cereals by electron-capture gas chromatography. Furthermore, it may also find application in being used as stationary phase in capillary column gas chromatography for determination of sterols.

Other Notes

We offer a variety of chromatography accessories including analytical syringes

Legal Information

SPB is a registered trademark of Merck KGaA, Darmstadt, Germany

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Determination of nivalenol and deoxynivalenol in cereals by electron-capture gas chromatography.
Scott PM
Journal - Association of Official Analytical Chemists, 69 (5), 889-893 (1985)
J M Jurado et al.
Talanta, 106, 14-19 (2013-04-20)
4-Methylsterols and 4,4-dimethylsterols of 47 samples of subcutaneous fat from Iberian pigs reared on two different fattening systems, "Extensive" and "Intensive", have been analyzed by GC-MS and GC-FID. The lipids were extracted by melting the subcutaneous fat in a microwave
V Gajdůsková et al.
Veterinarni medicina, 37(8), 471-478 (1992-08-01)
Occurrence of individual polychlorinated biphenyl (PCB) congeners in the environment, foodstuffs and other biological materials was assessed. Analysis of specific PCB congeners (28, 52, 101, 138, 153, 180), occurring in animal raw materials and foodstuffs most frequently, has been implemented.
O Suzuki et al.
Forensic science international, 46(3), 169-180 (1990-07-01)
Positive-ion electron impact (PIEI), positive-ion chemical ionization (PICI) and negative-ion chemical ionization (NICI) mass spectra of 9 carbamate pesticides are presented. In the PIEI mode, the spectra showed small molecular peaks, intense or base peaks due to M - CH3NHCO
Luisito Cercaci et al.
Journal of chromatography. A, 985(1-2), 211-220 (2003-02-13)
The sterol composition of extra virgin olive oil is very characteristic and, thus, has become a helpful tool to detect adulterations with other vegetable oils. Special attention has been addressed to the separate determination of the free and esterified sterol

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