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58201C40

Supelco

SUPELCOSIL LC-Diol HPLC Column

5 μm particle size, L × I.D. 25 cm × 4 mm

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eCl@ss:
32110501
NACRES:
SB.52

material

stainless steel column

Quality Level

agency

suitable for USP L20

product line

SUPELCOSIL

feature

endcapped: no

manufacturer/tradename

SUPELCOSIL

packaging

1 ea of

extent of labeling

3.5% carbon loading

parameter

≤70 °C temp. range
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable

L × I.D.

25 cm × 4 mm

surface area

170 m2/g

surface coverage

surface coverage 3.8 μmol/m2

matrix

silica gel, spherical particle platform
fully porous particle

matrix active group

diol phase

particle size

5 μm

pore size

120 Å pore size

pH range

2-7.5

application(s)

food and beverages

separation technique

hydrophilic interaction (HILIC)
normal phase
reversed phase

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This Item
5820158986C3058978C30
vibrant-m

58201

SUPELCOSIL LC-Diol HPLC Column

vibrant-m

58986C30

SUPELCOSIL LC-CN HPLC Column

matrix active group

diol phase

matrix active group

diol phase

matrix active group

cyano phase

matrix active group

C18 (octadecyl) phase

matrix

silica gel, spherical particle platform, fully porous particle

matrix

silica gel, spherical particle platform

matrix

silica gel, spherical particle platform, fully porous particle

matrix

silica gel, spherical particle platform, fully porous particle

pore size

120 Å pore size

pore size

120 Å pore size

pore size

120 Å

pore size

120 Å

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable

packaging

1 ea of

packaging

pkg of 1 ea

packaging

1 ea of

packaging

1 ea of

General description

LC-Diol columns can be used to separate proteins by gel filtration chromatography. When operated with an aqueous buffer, the diol phase can effectively shield the silica surface from interacting with proteins. A well-known use of diol columns, under normal phase conditions, is the separation of steroids and sterols.

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Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

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