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HPLC Analysis of Organic Acids on Ascentis® RP-Amide

application for HPLC

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HPLC: suitable

test parameters

column: Ascentis RP-Amide, 10 cm x 2.1 mm I.D., 3 μm particles (565301-U)
mobile phase: [A] 0.1% (v/v) TFA in [B] water
flow rate: 0.3 mL/min
column temp.: 35 °C
detector: UV, 210 nm
injection: 1 μL
sample: 0.2 - 4 g/L in mobile phase


application for HPLC


chemicals and industrial polymers
food and beverages
vitamins, nutraceuticals, and natural products

Analysis Note

This application demonstrates the suitability of Ascentis RP-Amide for the analysis of organic acids under 100% aqueous conditions.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany



    Citric acid
    Acrylic acid
    Fumaric acid

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