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HPLC Analysis of Sugars on SUPELCOSIL LC-NH2

application for HPLC

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HPLC: suitable

test parameters

column: SUPELCOSIL LC-NH2, 25 cm x 4.6 mm I.D., 5 μm particles (58338)
mobile phase: [A] water; [B] acetonitrile; (25:75, A:B)
flow rate: 1 mL/min
temp.: ambient
detector: refractive index (RI)
injection: 2 μL
sample: 10 mg/mL in water


application for HPLC


food and beverages

Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC



    Fructose, United States Pharmacopeia (USP) Reference Standard
    Dextrose, United States Pharmacopeia (USP) Reference Standard
    Sucrose, suitable for microbiology, ACS reagent, ≥99.0%
    Maltose solution, BioReagent, for molecular biology, ~20% in H2O
    Lactose, pharmaceutical secondary standard; traceable to USP and PhEur

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