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Showing 1-30 of 84 results for "08658" within Papers
Satoshi Wakita et al.
FEBS letters, 591(20), 3310-3318 (2017-08-24)
Mouse acidic mammalian chitinase (AMCase) degrades chitin with highest efficiency at pH 2.0 and is active up to pH 8.0. Here, we report that mouse AMCase also exhibits transglycosylation activity under neutral conditions. We incubated natural and artificial chitin substrates
Nico Lämmerhardt et al.
Carbohydrate research, 489, 107933-107933 (2020-02-13)
Various (semi)-synthetic non-digestible glucans (NDG) (resistant (malto)dextrin, isomaltooligosaccharide, soluble corn fiber, polydextrose) were analyzed by methylation analysis, 1H NMR spectroscopy, ESI-MSn, TLC, and capillary electrophoresis (CE/UV). Partly complementary and partly corroborative results were obtained by these methods yielding a comprehensive
Jessica Evans et al.
Cognitive and behavioral neurology : official journal of the Society for Behavioral and Cognitive Neurology, 28(1), 17-26 (2015-03-27)
Up to half of patients with amyotrophic lateral sclerosis (ALS) may have cognitive difficulty, but most cognitive measures are confounded by a motor component. Studies relating impaired cognition in ALS to disease in gray matter and white matter are rare.
Ding-Tao Wu et al.
Carbohydrate polymers, 134, 12-19 (2015-10-03)
Water-soluble polysaccharides from 51 batches of fruits of L. barbarum (wolfberry) in China were investigated and compared using saccharide mapping, partial acid hydrolysis, single and composite enzymatic digestion, followed by polysaccharide analysis by using carbohydrate gel electrophoresis (PACE) analysis and
Yong Deng et al.
Carbohydrate polymers, 199, 619-627 (2018-08-26)
Qualitative and quantitative analysis of non-starch polysaccharide (NSP) from Pseudostellaria heterophylla in China (Guizhou, Anhui and Fujian) were performed using high performance size exclusion chromatography coupled with multi-angle laser light scattering and refractive index detector (HPSEC-MALLS-RID), saccharide mapping based on
Carmen L Delgado et al.
Biochimica et biophysica acta, 1848(4), 895-906 (2015-01-03)
In a previous study, it was shown that purified preS domains of hepatitis B virus (HBV) could interact with acidic phospholipid vesicles and induce aggregation, lipid mixing and leakage of internal contents which could be indicative of their involvement in
Lisa Byrge et al.
Human brain mapping, 41(9), 2249-2262 (2020-03-10)
Despite enthusiasm about the potential for using fMRI-based functional connectomes in the development of biomarkers for autism spectrum disorder (ASD), the literature is full of negative findings-failures to distinguish ASD functional connectomes from those of typically developing controls (TD)-and positive
Mykola V Rodnin et al.
The Journal of membrane biology, 253(3), 287-298 (2020-06-06)
Protegrin-1 (PG-1), an 18-residue β-hairpin stabilized by two disulfide bonds, is a member of a family of powerful antimicrobial peptides which are believed to act through membrane permeabilization. Here we used a combination of experimental and computational approaches to characterize
Rachida Abes et al.
Nucleic acids research, 36(20), 6343-6354 (2008-09-18)
Redirecting the splicing machinery through the hybridization of high affinity, RNase H- incompetent oligonucleotide analogs such as phosphoramidate morpholino oligonucleotides (PMO) might lead to important clinical applications. Chemical conjugation of PMO to arginine-rich cell penetrating peptides (CPP) such as (R-Ahx-R)(4)
Nataliya M Samoshina et al.
Pharmaceutics, 3(3), 379-405 (2011-01-01)
Incorporation of a pH-sensitive conformational switch into a lipid structure enables a drastic conformational flip upon protonation that disrupts the liposome membrane and causes rapid release of cargo specifically in areas of increased acidity. pH-sensitive liposomes containing the amphiphile (1)
Panhong Yuan et al.
Carbohydrate polymers, 129, 194-200 (2015-06-09)
High-molecular-mass hyaluronan (HA) was controllably depolymerized in pure aqueous solution with recombinant leech hyaluronidase (HAase). The HAase concentration per unit HA and hydrolysis time played important roles in molecular mass distribution. By modulating the concentrations of HAase and controlling the
Thitiporn Anunthawan et al.
Biochimica et biophysica acta, 1848(6), 1352-1358 (2015-03-15)
We investigated the mechanisms of two tryptophan-rich antibacterial peptides (KT2 and RT2) obtained in a previous optimization screen for increased killing of both Gram-negative and Gram-positive bacteria pathogens. At their minimal inhibitory concentrations (MICs), these peptides completely killed cells of
Byung-Ju Jin et al.
Lab on a chip, 17(5), 887-895 (2017-02-12)
Water transport across epithelial monolayers is of central importance in mammalian fluid homeostasis, and epithelial aquaporin (AQP) water channels are potential drug targets. Current methods to measure transepithelial water permeability based on indicator dilution have limited accuracy and can require
Daniel Tello et al.
The FEBS journal, 281(11), 2558-2569 (2014-04-05)
We have used an isolated chimeric protein E1340 E2661 that includes the ectodomains of the envelope proteins of hepatitis C virus to study its interaction with model membranes. E1340 E2661 has some of the membrane destabilization properties, vesicle aggregation, lipid
Chen Fan et al.
Nature, 580(7802), 288-293 (2020-04-10)
Inactivation is the process by which ion channels terminate ion flux through their pores while the opening stimulus is still present1. In neurons, inactivation of both sodium and potassium channels is crucial for the generation of action potentials and regulation
Wanchen Shao et al.
Plant methods, 16, 99-99 (2020-08-04)
Identification and characterization of key enzymes associated with cell wall biosynthesis and modification is fundamental to gain insights into cell wall dynamics. However, it is a challenge that activity assays of glycosyltransferases are very low throughput and acceptor substrates are
Sree Devi Menon et al.
The Journal of cell biology, 169(6), 909-920 (2005-06-16)
In Drosophila, myoblasts are subdivided into founders and fusion-competent myoblasts (fcm) with myotubes forming through fusion of one founder and several fcm. Duf and rolling pebbles 7 (Rols7; also known as antisocial) are expressed in founders, whereas sticks and stones
Eugenio Notomista et al.
Microbial cell factories, 14, 126-126 (2015-09-05)
Pathogenic bacteria easily develop resistance to c onventional antibiotics so that even relatively new molecules are quickly losing efficacy. This strongly encourages the quest of new antimicrobials especially for the treatment of chronic infections. Cationic antimicrobial peptides (CAMPs) are small
Øyvind Strømland et al.
Biochimica et biophysica acta, 1859(5), 1029-1039 (2017-01-11)
Helix A and -C of α-lactalbumin, a loosely folded amphitropic protein, perturb lipid monolayers by the formation of amyloid pore-like structures. To investigate whether these helices are able to disrupt fully formed bilayers, we designed peptides comprised of Helix A
Adam Z Spitz et al.
Nature communications, 12(1), 1134-1134 (2021-02-20)
The BCL-2 family protein BAX has essential activity in mitochondrial regulation of cell death. While BAX activity ensures tissue homeostasis, when dysregulated it contributes to aberrant cell death in several diseases. During cellular stress BAX is transformed from an inactive
Akinori Kashimura et al.
PloS one, 8(11), e78669-e78669 (2013-11-19)
Acidic mammalian chitinase (AMCase) has been shown to be associated with asthma in mouse models, allergic inflammation and food processing. Here, we describe an E. coli-expression system that allows for the periplasmic production of active AMCase fused to Protein A
Geoffray Leriche et al.
Scientific reports, 9(1), 19359-19359 (2019-12-20)
A major challenge in liposomal research is to minimize the leakage of encapsulated cargo from either uncontrolled passive permeability across the liposomal membrane or upon fusion with other membranes. We previously showed that liposomes made from pure Archaea-inspired bipolar tetraether
Samir E L Andaloussi et al.
Nucleic acids research, 39(9), 3972-3987 (2011-01-20)
While small interfering RNAs (siRNAs) have been rapidly appreciated to silence genes, efficient and non-toxic vectors for primary cells and for systemic in vivo delivery are lacking. Several siRNA-delivery vehicles, including cell-penetrating peptides (CPPs), have been developed but their utility
Akira Tawada et al.
Glycobiology, 12(7), 421-426 (2002-07-18)
Hyaluronan (HA) was depolymerized by partial digestion with testicular hyaluronidase and separated into size-uniform HA oligosaccharides from 4-mers to 52-mers by anion exchange chromatography after removal of the hyaluronidase. The purity and size of each HA oligosaccharide was confirmed by
Masaya Misaki et al.
Magnetic resonance in medicine, 74(6), 1609-1620 (2014-12-24)
In order to more precisely differentiate cerebral structures in neuroimaging studies, a novel technique for enhancing the tissue contrast based on a combination of T1-weighted (T1w) and T2-weighted (T2w) MRI images was developed. The combined image (CI) was calculated as
Crystal M Darby et al.
PloS one, 8(7), e68942-e68942 (2013-08-13)
Bacterial pathogens like Mycobacterium tuberculosis (Mtb) encounter acidic microenvironments in the host and must maintain their acid-base homeostasis to survive. A genetic screen identified two Mtb strains that cannot control intrabacterial pH (pHIB) in an acidic environment; infection with either
Martijn Riool et al.
Biochimica et biophysica acta. Biomembranes, 1862(8), 183282-183282 (2020-05-08)
Antimicrobial peptides are considered promising candidates for the development of novel antimicrobial agents to combat infections by multi-drug-resistant (MDR) bacteria. Here, we describe the identification and characterization of the synthetic peptide TC19, derived from the human thrombocidin-1-derived peptide L3. Biophysical
Radda Rusinova et al.
The Journal of general physiology, 138(2), 249-270 (2011-07-27)
The thiazolidinediones (TZDs) are used in the treatment of diabetes mellitus type 2. Their canonical effects are mediated by activation of the peroxisome proliferator-activated receptor γ (PPARγ) transcription factor. In addition to effects mediated by gene activation, the TZDs cause
Guotian Li et al.
BMC biotechnology, 18(1), 54-54 (2018-09-06)
Switchgrass (Panicum virgatum L.) is a promising bioenergy feedstock because it can be grown on marginal land and produces abundant biomass. Recalcitrance of the lignocellulosic components of the switchgrass cell wall to enzymatic degradation into simple sugars impedes efficient biofuel
Jessica A Bernard et al.
Frontiers in neuroanatomy, 6, 31-31 (2012-08-22)
The cerebellum plays a role in a wide variety of complex behaviors. In order to better understand the role of the cerebellum in human behavior, it is important to know how this structure interacts with cortical and other subcortical regions
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