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Showing 1-30 of 54 results for "10417" within Papers
Gabriel Gibrat et al.
Biochimica et biophysica acta, 1844(9), 1560-1568 (2014-05-28)
Apo-calmodulin, a small soluble mainly α protein, is a calcium-dependent protein activator. Calcium binding affects the calmodulin conformation but also its stability. Calcium free form unfolds between 40 and 80°C, whereas the calcium-saturated form is stable up to temperatures as
Rebecca L Smathers et al.
PloS one, 7(6), e38459-e38459 (2012-06-16)
4-Hydroxynonenal (4-HNE) is a reactive α,β-unsaturated aldehyde produced during oxidative stress and subsequent lipid peroxidation of polyunsaturated fatty acids. The reactivity of 4-HNE towards DNA and nucleophilic amino acids has been well established. In this report, using proteomic approaches, liver
Beiwen Zheng et al.
PloS one, 6(5), e19510-e19510 (2011-05-26)
The scavenging ability of sufficient divalent metal ions is pivotal for pathogenic bacteria to survive in the host. ATP-binding cassette (ABC)-type metal transporters provide a considerable amount of different transition metals for bacterial growth. TroA is a substrate binding protein
Stefan Kofler et al.
Journal of molecular biology, 422(1), 109-123 (2012-05-29)
The ability of pathogenesis-related proteins of family 10 to bind a broad spectrum of ligands is considered to play a key role for their physiological and pathological functions. In particular, Bet v 1, an archetypical allergen from birch pollen, is
L D'Alfonso et al.
Biochimica et biophysica acta, 1432(2), 194-202 (1999-07-17)
Steady-state and dynamic fluorescence titrations show that: (a) the complex between beta-lactoglobulin (BLG) and 1-anilinonaphthalene-8-sulfonate (ANS) displays a heterogeneous equilibrium with large changes in the binding strength vs. pH and ion concentration; and (b) the fluorescence response of bound ANS
Chemiluminescence enzyme immunoassay of 17 alpha-hydroxyprogesterone using glucose oxidase and bis(2,4,6-trichlorophenyl) oxalate - fluorescent dye system.
H Arakawa et al.
Chemical & pharmaceutical bulletin, 30(8), 3036-3039 (1982-08-01)
pH-Responsive Micellization of Amphiphilic Diblock Copolymers Synthesized via Reversible Addition-Fragmentation Chain Transfer Polymerization
Yusa, S.-i., et al.
Macromolecules, 36, 4208-4208 (2003)
A C Saucier et al.
Biochemistry, 24(26), 7581-7585 (1985-12-17)
The binding of 8-anilino-1-naphthalenesulfonate (ANS) to ciliary dynein ATPase leads to a marked increase in the dye's fluorescence intensity, accompanied by a blue shift in the observed fluorescence emission maximum. We found that dynein has 37 +/- 3 ANS binding
Oktay K Gasymov et al.
Biochemistry, 47(5), 1414-1424 (2008-01-09)
Human tear lipocalin (TL) exhibits diverse functions, most of which are linked to ligand binding. To map the binding site of TL for some amphiphilic ligands, we capitalized on the hydrophobic and hydrophilic properties of 8-anilino-1-naphthalenesulfonic acid (ANS). In single
Cyclodextrins Modified with Polymer Chains Which Are Responsive to External Stimuli
Nozaki, T., et a.
Macromolecules, 28, 522-522 (1995)
Dimitra Georgiadou et al.
PloS one, 6(11), e27037-e27037 (2011-11-10)
Apolipoprotein E (apoE) is a major protein of the lipoprotein transport system that plays important roles in lipid homeostasis and protection from atherosclerosis. ApoE is characterized by structural plasticity and thermodynamic instability and can undergo significant structural rearrangements as part
M Collini et al.
Protein science : a publication of the Protein Society, 9(10), 1968-1974 (2000-12-06)
The fluorescence time decay parameters of the beta-lactoglobulin-1-anilinonaphthalene-8-sulfonate complex have been investigated under physical and chemical perturbations (2 < pH < 8 and added electrolyte 0 < NaCl < 0.5 M) to obtain new insight on the nature of the
Chang Li et al.
International journal of biological macromolecules, 49(5), 910-916 (2011-08-23)
In our study, we showed that at a relatively low concentration, H(2)O(2) can irreversibly inactivate the human brain type of creatine kinase (HBCK) and that HBCK is inactivated in an H(2)O(2) concentration-dependent manner. HBCK is completely inactivated when incubated with
W P Le et al.
Journal of biochemistry, 119(4), 674-679 (1996-04-01)
Under conditions of low pH, the conformational states of holo-YADH and apo-YADH were examined by protein intrinsic fluorescence, ANS fluorescence, and far-UV CD measurements. The results obtained show that a low ionic strength, with the addition of HCl, the holo-
Tianlin Wang et al.
Journal of chromatography. A, 987(1-2), 485-492 (2003-03-05)
An indirect capillary electrophoresis (CE) method was developed based on two competitive chemical equilibria for determining the stability constant of an inclusion complex formed between a cyclodextrin and a solute. 8-Anilino-1-naphthalenesulfonic acid was employed as a fluorescence probe. A linear
Sandeep Dave et al.
International journal of biological macromolecules, 49(4), 536-542 (2011-07-09)
Stem bromelain (SBM) is a therapeutic protein that has been studied for alkaline denaturation in the intestines, the principal site of its absorption. In this study, we investigated fluorinated alcohol 2,2,2-trifluoroethanol (TFE)-induced conformational changes in the specific/pre-molten globule (SMG) state
Maddalena Collini et al.
Protein science : a publication of the Protein Society, 12(8), 1596-1603 (2003-07-24)
The use of spectroscopy in the study of fatty acids binding to bovine beta-lactoglobulin (BLG) appears to be a difficult task, as these acid compounds, assumed as the protein natural ligands, do not exhibit favorable optical response such as, for
V G Artyukhov et al.
Bulletin of experimental biology and medicine, 153(6), 907-911 (2012-11-01)
Structural and functional state of human blood lymphocytes after exposure to UV light (240-390 nm) in doses of 151-1359 J/m(2) was studied by methods of laser flow cytofluorometry, indirect immunofluorescence, and fluorescent probes. Using a combination of these methods, we
Irem Nasir et al.
PloS one, 10(8), e0136687-e0136687 (2015-08-28)
The interactions of biological macromolecules with nanoparticles underlie a wide variety of current and future applications in the fields of biotechnology, medicine and bioremediation. The same interactions are also responsible for mediating potential biohazards of nanomaterials. Some applications require that
Shruti Arya et al.
Physical chemistry chemical physics : PCCP, 17(35), 22862-22871 (2015-08-13)
The self-assembly of proteins triggered by a conformational switch into highly ordered β-sheet rich amyloid fibrils has captivated burgeoning interest in recent years due to the involvement of amyloids in a variety of human diseases and a diverse range of
James A McLure et al.
Drug metabolism and disposition: the biological fate of chemicals, 39(9), 1711-1717 (2011-05-26)
The fluorescence of 1-anilinonaphthalene-8-sulfonate (ANS) in the presence of human liver microsomes (HLMs) is altered by drugs that bind nonspecifically to the lipid bilayer. The present study characterized the relationship between the nonspecific binding (NSB) of drugs to HLMs as
The use of fluorescent probes for the study of membranes.
A Azzi
Methods in enzymology, 32, 234-246 (1974-01-01)
Clare A Peters Libeu et al.
PloS one, 7(6), e40027-e40027 (2012-07-07)
One of the events associated with Alzheimer's disease is the dysregulation of α- versus β-cleavage of the amyloid precursor protein (APP). The product of α-cleavage (sAPPα) has neuroprotective properties, while Aβ1-42 peptide, a product of β-cleavage, is neurotoxic. Dimerization of
Fluorescence properties of carbazolyl-containing amphiphilic copolymers
Rutkaite, R., et al.
J. Photochem. Photobiol., A, 138, 245-245 (2001)
Gulam Rabbani et al.
Cell biochemistry and biophysics, 61(3), 551-560 (2011-08-13)
Acid unfolding pathway of conalbumin (CA), a monomeric glycoprotein from hen egg white, has been investigated using far- and near-UV CD spectroscopy, intrinsic fluorescence emission, extrinsic fluorescence probe 1-anilino-8-napthalene sulfonate (ANS) and dynamic light scattering (DLS). We observe pH-dependent changes
Silvia Zorrilla et al.
Methods in molecular biology (Clifton, N.J.), 952, 237-252 (2012-10-27)
Transcription factors of the peroxisome proliferator-activated receptor (PPAR) family are ligand-activated receptors that play key roles in lipid metabolism and inflammation. The γ isoform (PPARγ) is involved in adipocyte differentiation, insulin sensitization, and vascular pathophysiology, including inflammation and atherosclerosis, for
Kevin C Hadley et al.
Cell stress & chaperones, 16(5), 549-561 (2011-04-13)
The inability of cells to maintain protein folding homeostasis is implicated in the development of neurodegenerative diseases, malignant transformation, and aging. We find that multiphoton fluorescence imaging of 1-anilinonaphthalene-8-sulfonate (ANS) can be used to assess cellular responses to protein misfolding
Shilpi Gupta et al.
Small (Weinheim an der Bergstrasse, Germany), 9(6), 894-904 (2012-12-12)
A new class of non-ionic dendronized multiamphiphilic polymers is prepared from a biodegradable (AB)n-type diblock polymer synthesized from 2-azido-1,3-propanediol (azido glycerol) and polyethylene glycol (PEG)-600 diethylester using Novozym-435 (Candida antarctica lipase) as a biocatalyst, following a well-established biocatalytic route. These
William J Andrews et al.
Molecular and cellular biochemistry, 371(1-2), 217-223 (2012-09-05)
IQGAPs are cytoskeletal scaffolding proteins which collect information from a variety of signalling pathways and pass it on to the microfilaments and microtubules. There is a well-characterised interaction between IQGAP and calmodulin through a series of IQ-motifs towards the middle
Fluorescence and Conductivity Studies of Polyelectrolyte-Induced Aggregation of Alkyltrimethylammonium Bromides
Kogej, K., and Skerjancc, J.
Langmuir, 15, 4251-4251 (1999)
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