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Growth Characteristics of the expresSFplus Serum-Free Insect Cell Line in EX-CELL420 Serum-Free Media
at higher
densities. Cell densities in EX-CELLTM 420 were typically
around 6 x 106 cells/mL (range 3.7 x 106 to
9.9 x 106 cells/mL) and displayed an average doubling time
of 26 hours. Culture viabilities
EX-CELL 302 Serum-Free Medium for CHO Cells
3. Centrifuge the cells at 200 g for 5 minutes. Remove the
supernatant.
4. Resuspend the cells in the freezing medium at 5 x 106 to
1 x 107 cells/mL.
5. Rapidly transfer 1 - 2 mL of this suspension
EX-CELL NS0- An Animal-Component Free
Density
(Cells/mL)
Average
% Viability
Average Doubling
Time (Hours)
2.2 x 106
1.2 x 106
2.9 x 106
1.9 x 106
95.5
84.5
92.0
92.6
16.1
20.0
18.6
22.2
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Product Information Sheet - 14591C
shaker
flasks?
The average cell density achieved during a 3-day passage is
approximately 2.9 x 106 ± 1 x 106 cells/mL in shaker flasks.
What is the average doubling time of HeLa cells
in EX-CELLTM HeLa
Suspension MDCK Cell Culture in EX-CELL MDCK Serum-Free Medium
approximately 1-3 x 106 cells/mL, with culture viabilities in
excess of 93%, when subcultured every 3 - 4 days (Figure 2).
The growth curve (Figure 3) indicates that maximal cell
densities nearing 5 x 106 cell/mL
MILLIPLEX®MAP Human Myokine Panel is an optimized quantitative immunoassay that simultaneously measures 15 novel muscle-secreted factors
29 122 27
20 1115 16 16 113 43 27 100 15 44 26 35 30 133 27
std
5
20 3985 17 16 113 43 30 95 14 44 27 33 31 135 26
20
Data sheet
LabRoll™ 1 Roll 5M × 115 mm
IST-106-078TS Trial LabSheet™ 5 Sheets 125 mm × 78 mm
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ChemiScreen™ Flash Aequorin Calcium-Optimized Stable Cell Line Human Recombinant EP2 Prostanoid Receptor
prepared as in Step 4). Centrifuge cells at 200 x g
for 5 min. Resuspend cells at 5 x 106 cells/mL in Freezing Media (cell densities of 2-10
x 106 are also acceptable if necessary). Dispense 1
ChemiScreenTM GLOW AEQUORIN CALCIUM-OPTIMIZED STABLE CELL LINE HUMAN RECOMBINANT M1 MUSCARINIC ACETYLCHOLINE RECEPTOR
prepared as in Step 4). Centrifuge cells at 200 x g for
5 min. Resuspend cells at 5 x 106 cells/mL in Freezing Media (cell densities of 2-10 x
106 are also acceptable if necessary). Dispense 1
ChemiScreen™ FLASH AEQUORIN CALCIUM-OPTIMIZED STABLE CELL LINE HUMAN RECOMBINANT M1 MUSCARINIC ACETYLCHOLINE RECEPTOR
prepared as in Step 4). Centrifuge cells at 200 x g for
5 min. Resuspend cells at 5 x 106 cells/mL in Freezing Media (cell densities of 2-10 x
106 are also acceptable if necessary). Dispense 1
ChemiScreen™ Calcium-Optimized Stable Cell Line Human Recombinant sst<sub>2 </sub>Receptor
prepared as in Step 4). Centrifuge cells
at 200 x g for 5 min. Resuspend cells at 5 x 106 cells/mL in Chem-1 Freezing
Media (cell densities of 2-10 x 106 are also acceptable if necessary). Dispense
EX-CELL 610-HSF Serum-Free Medium for Hybridoma Cells
3. Centrifuge the cells at 200 g for 5 minutes. Remove the
supernatant.
4. Resuspend the cells in the freezing medium at 5 x 106 to
1 x 107 cells/mL.
5. Rapidly transfer 1 - 2 mL of this suspension
Product Information Sheet - 14591C
served as controls). The
plates were incubated at 37 C, 5% CO2 for 10 days, then
observed on an inverted microscope for CPE.
Viable Cells/mL
(x 106)
Percent Viable Doubling Time
(Hrs)
Average
Standard
EX-CELL 420 Serum-Free Medium for Insect Cells
3. Centrifuge the cells at 200 g for 5 minutes. Remove the
supernatant.
4. Resuspend the cells in the freezing medium at 5 x 106 to
1 x 107 cells/mL.
5. Rapidly transfer 1 - 2 mL of this suspension
EX-CELL 302 Serum-Free Medium for CHO Cells
3. Centrifuge the cells at 200 g for 5 minutes. Remove the
supernatant.
4. Resuspend the cells in the freezing medium at 5 x 106 to
1 x 107 cells/mL.
5. Rapidly transfer 1 - 2 mL of this suspension
EX-CELL CD CHO- A Chemically DefinedAnimal-Component Free Serum-Free Mediumfor CHO Cells
EX-CELLTM CD CHO at a density of
5 x 105 cells/mL. Cells were subcultured every 3 - 4 days and
were considered fully adapted when cell densities achieved
> 2.0 x 106 cells/mL and viabilities of > 95%.
ChemiScreen™ Flash Aequorin Calcium-Optimized Stable Cell Line Human Recombinant EP3 Prostanoid Receptor
prepared as in Step 4). Centrifuge cells at 200 x g
for 5 min. Resuspend cells at 5 x 106 cells/mL in Freezing Media (cell densities of 2-10
x 106 are also acceptable if necessary). Dispense 1
ChemiScreen™ Glow Aequorin Calcium-Optimized Stable Cell Line Human Recombinant TP Prostanoid Receptor
prepared as in Step 4). Centrifuge cells at 200 x g
for 5 min. Resuspend cells at 5 x 106 cells/mL in Freezing Media (cell densities of 2-10
x 106 are also acceptable if necessary). Dispense 1
EX-CELL 620-HSF Serum-Free Medium for Hybridoma Cells
3. Centrifuge the cells at 200 g for 5 minutes. Remove the
supernatant.
4. Resuspend the cells in the freezing medium at 5 x 106 to
1 x 107 cells/mL.
5. Rapidly transfer 1 - 2 mL of this suspension
EX-CELL 420 Frequently Asked Questions
dimethyl
sulfoxide (DMSO). Centrifuge the cells at 200 g for 5 minutes.
Remove the supernatant. Resuspend the cells in the freezing
medium at 5 x 106 to 1 x 107 cells/mL. Rapidly transfer
1 - 2 mL of this
EX-CELL 420 Serum-Free Medium for Insect Cells
3. Centrifuge the cells at 200 g for 5 minutes. Remove the
supernatant.
4. Resuspend the cells in the freezing medium at 5 x 106 to
1 x 107 cells/mL.
5. Rapidly transfer 1 - 2 mL of this suspension
User Guide
SA. Biochim biophys acta.,
1860(6):1098-106 (2016).
2. Jensen MA, et al. PLoS ONE, 5(6):e11024.
(2010).
3. Rees WA, et al. Biochem., 32(1):137-44. (1993).
4. Kostic T, et al. Appl Microbiol
B6-WHITE™ MURINE ES CELL LINE
____________________
CATALOG NUMBER:
SCR011 QUANTITY: 5 x 106 cells, passage 10
LOT NUMBER:
CONCENTRATION: 2.5 x 106 cells / vial
BACKGROUND:
The generation
ChemiScreen™ CALCIUM-OPTIMIZED STABLE CELL LINE HUMAN RECOMBINANT TSH RECEPTOR, GLYCOPROTEIN HORMONE RECEPTOR FAMILY
prepared as in Step 4). Centrifuge cells
at 200 x g for 5 min. Resuspend cells at 5 x 106 cells/mL in Chem-10 Freezing
Media (cell densities of 2-10 x 106 are also acceptable if necessary). Dispense
ChemiScreen™ Calcium-Optimized Stable Cell Line Human Recombinant GPR109A Receptor
prepared as in Step 4). Centrifuge cells at 200 x g for
5 min. Resuspend cells at 5 x 106 cells/mL in 1321N1 Freezing Media (cell densities of
2-10 x 106 are also acceptable if necessary). Dispense
EX-CELL 610-HSF Serum-Free Medium for Hybridoma Cells
3. Centrifuge the cells at 200 g for 5 minutes. Remove the
supernatant.
4. Resuspend the cells in the freezing medium at 5 x 106 to
1 x 107 cells/mL.
5. Rapidly transfer 1 - 2 mL of this suspension
ChemiScreen™ Human Recombinant XCR1 Chemokine Receptor Calcium-Optimized Stable Cell Line
prepared as in Step 4). Centrifuge cells
at 200 x g for 5 min. Resuspend cells at 5 x 106 cells/mL in Chem-4 Freezing
Media (cell densities of 2-10 x 106 are also acceptable if necessary). Dispense
EX-CELL 405 Serum-Free Medium for Insect Cells
3. Centrifuge the cells at 200 g for 5 minutes. Remove the
supernatant.
4. Resuspend the cells in the freezing medium at 5 x 106 to
1 x 107 cells/mL.
5. Rapidly transfer 1 - 2 mL of this suspension
EX-CELL 293 Serum-Free Medium for HEK 293 Cells FAQs
Cell density Minimum cell density Minimum cell density
Promotion determination of 2 - 4 x 106 cells/mL of 2 - 4 x 106 cells/mL
with HEK 293 cells with HEK 293 cells
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Technical
ChemiScreen™ Glow Aequorin Calcium-Optimized Stable Cell Line Human Recombinant H1 Histamine Receptor
prepared as in Step 4). Centrifuge cells at 200 x g for 5 min.
Resuspend cells at 5 x 106 cells/mL in Freezing Media (cell densities of 2-10 x 106 are also
acceptable if necessary). Dispense 1
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