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RNA-Binding Protein Immunoprecipitation
Anti-4E-BP1, Rabbit Monoclonal 04-321
Anti-Ago1 07-599
Anti-Ago1, clone 6D8.2 04-083
Anti-Ago2 07-590
Anti-Ago2, clone 9E8.2 04-642
anti-Ago2, clone 9E8.2 04-084
Anti-Ago4, clone 5F9.2
Product Information Sheet-rdlig-ro
Sma I + Adaptor
Lambda gt 11 Arms
+ Insert/EcoR I
5 × 107 4.9 × 104 1 × 107
https://www.sigmaaldrich.com
11
21
.0
49
The Effective Use of Protein Kinase Inhibitors
139 92 92 107 85 85 89 90 114 113 90 107 102 97 98 107
JNK1α1/SAPK1c 97 98 96 49 104 96 102 111 101 93 97 108 101 98 92 89 95 91 100
JNK/SAPK1c
The Effective Use of Protein Kinase Inhibitors
139 92 92 107 85 85 89 90 114 113 90 107 102 97 98 107
JNK1α1/SAPK1c 97 98 96 49 104 96 102 111 101 93 97 108 101 98 92 89 95 91 100
JNK/SAPK1c
Data Sheet - C6395 - Lot 71K88051
Ionization Mass Spectrum
Abundance
43
67 91 107
121
147
174 193
207
231
246
271 299
314
50 100 150 200 250 300
0
4000
8000
12000
Product Information Sheet - M4507
Biochem. J., 131(1),
107-117 (1973).
2. Coleman, P. L., et al., Some sensitive methods for
the assay of trypsinlike enzymes. Meth. Enzymol.,
45, 12-26 (1976).
MES/CRF 1/04
Sigma
Product Information Sheet - P2870
et al., Immunopathogenesis of
environmentally induced lupus in mice.
Environ. Health Perspect., 107, 723-7 (1999).
7. Holmdahl, R., et al., Arthritis induced in rats with
nonimmunogenic adjuvants
Data Sheet - A8535
130 140 150 160 170 180 190 200 210 220 230
0
5000
10000
15000
20000
25000
30000
35000
m/z
Abundance
42
58
63 77 91 101 107 115
Product Information Sheet - M8883
Product Number M 8883
Storage Temperature -0 °C
Product Description
Molecular Formula: C10H9O6P
Molecular Weight: 256.2
CAS Number: 3368-04-5
Melting Point: 214-216 °C1
Specific Rotation
Application Note - qPCR Validation of Candidates for Modulators of the MAPkinase Signaling Pathway
Strand cDNA Synthesis Kit 1 kit for 50 reactions 04 379 012 001
Order
INFO
45/45a 6/6b 7/14a 0/0b
a b
Figure 3: Overlap of number of genes and assays detected in dual- and single-color
assays
Mycoplasma clearance by Millipore Express SHR filters at pilot and production scales
-scale)
290 1.10E+05 2000 2.20E+08 7.59E+05 0 ≥ 8.3
3 x 30 in.
cartridge filters
(production-scale)
44,100 1.10E+04 200,000 2.20E+09 4.99E+04 0 ≥ 7.0
References
1. Acholeplasma
Product Information Sheet: Cellvento® BHK-200 cell culture medium
tubes in
Cellvento® BHK-200 medium, 10% DMSO,
1 × 107 cells/mL)
• Prepre a WCB (min. 100 tubes in
Cellvento® BHK-200 medium, 10% DMSO,
1 × 107 cells/mL)
Product Information Sheet - 11758241001
ml
each)
04 693 116 001
cOmplete, Mini 30 tablets in foil
blisters, (for 10 ml
each)
04 693 124 001
cOmplete, EDTA-free 20 tablets in foil
blisters, (for 50 ml
each)
04 693 132
Dental Application Brochure
-01-0
Nitric Acid 7697-37-2
Phosphoric Acid Meets analytical
specification of
Ph. Eur., BP, NF, FCC
7664-38-2
Sulfuric Acid 7664-93-9
Dental Solvents
(±)-1,3-Butanediol 107-88-0
BioTracker Orange-NHS Live Cell pH Dye
www.millipore.com/publicationrewards to learn more!
Data Sheet
page 1 of 2
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Sterilizing-Grade Aervent®Filters
Code
0 = (2-222) O-ring
7 = (2-226) O-ring with
locking tab and spear
Pore Size
GR = 0.2 μm
Pore Size
GR = 0.2 μm
Length Size
10 = Opticap® XL 10
Chrysamine G and BTA-1: New probes forB-amyloid aggregates
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LABORATORY NOTEBOOK ISSUANCE PAGE
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LABORATORY NOTEBOOK ISSUANCE PAGE
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Data Sheet - S6448 - Lot 031M0741
glycerol.
Molecular mass: ∼100 kDa
Purity: ≥70% (SDS-PAGE, see Figure 1)
Specific Activity: 79–107 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This product is for R&D use only, not for
Shaping Epigenetics Discovery - Epigenetics Product Selection Brochure
using the PAbP1 antibody (04-1467) and
HRP-goat-anti-mouse igG.
Description Catalogue No.
Anti-4E-BP1, Rabbit Monoclonal 04-321
Anti-Ago1 07-599
Anti-Ago1, clone 6D8.2 04-083
Anti-Ago2 07-590
LABORATORY NOTEBOOK ISSUANCE PAGE
.
24
(1
45
)
15
0.
36
15
1.
96
15
7.
25
23
2.
04
23
1.
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23
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03
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58
.6
Product Information Sheet-tripure-ro
04
21
.0
50
40
92
20
01
9
https://www.sigmaaldrich.com
Roche Diagnostics GmbH
Sandhofer Strasse 116
68305 Mannheim
Germany
6. Supplementary Information
04
21
Product Information Sheet-04719956001
analysis.
(1) Cells grown in 100 mm plates typically contain 107 cells (50 mg). The typical yield resulting from the extraction of 107 cells is
approximately 3 mg of total protein.
Product Information Sheet - F4428
and ANTI-FLAG M2
required may be different and may need calibration.
Assay
1. Collect 2-3 x 107 Jurkat cells by centrifugation and
resuspend the cells in Jurkat growth medium at
approximately
Product Information Sheet-04719964001
analysis.
(1) Cells grown in 100 mm plates typically contain 107 cells (50 mg). The typical yield resulting from the extraction of 107 cells is
approximately 3 mg of total protein.
Lysis
Product Information Sheet - CS0760
GE Healthcare.
EB,MAM 04/18-1
Appendix
Figure 1.
Typical Mitochondria Staining using JC-1 Stain in a Multiwell Plate Format
JC-1 staining assay
0
1000
2000
3000
User Guide - 11741985001
1) have to be added to get the required final volume
of 400 µl.
Number of cells 1 × 108 2 × 107 1 × 107 2 × 106 2 × 105
Amount of total
RNA
500 �g
Reprogramming Application Note Human Non-inducible OSLN
p24 (ng/ml) TU Titer (TU/ml)
hOct4 6.64 0.99 × 106
hSox2 68.8 1.02 × 107
hNanog 82.8 1.23 × 107
hLin28 68.8 1.02 × 107
Protein Expression Validation
To demonstrate that each virus
Magna Nuclear RIP¿ (Native) Nuclear RNA-Binding Protein Immunoprecipitation Kit
/ U1
1
10
100
1000
1.00E+06 2.00E+05 4.00E+04 1.00E+04 5.00E+03
Signal/Noise Ratio (Crosslink)
NEAT1/U1
0
50
100
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