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The Effective Use of Protein Kinase Inhibitors
92 86 100 87 75 106 81 25 109 95 101 6 32 18
GSK-3β 107 92 90 13 38 105 83 101 66 61 85 53 30 89 58 99 5 50 46
ROCK-II 0 13 7
The Effective Use of Protein Kinase Inhibitors
92 86 100 87 75 106 81 25 109 95 101 6 32 18
GSK-3β 107 92 90 13 38 105 83 101 66 61 85 53 30 89 58 99 5 50 46
ROCK-II 0 13 7
Product Information Sheet-11814770001
RNase
Solution
[μl]
Protein
Precipitation
Solution [ml] *)
1 × 107 3 2 100 1.2
2 × 107 6 4 200 2.5
3 × 107 9 6 300 3.7
4 × 107 12 8 400 5
*) Calculate the volume
MILLIPLEX®MAP Human Myokine Panel is an optimized quantitative immunoassay that simultaneously measures 15 novel muscle-secreted factors
22 30 28 134 26
std
6
20 11 16 15 110 42 4888 95 15 43 25 31 29 134 25
18 11 16 17 107 42 5039 94 13 43 24 31 29 133 25
Product Information Sheet-MITOISO2
Repeat the Wash Step once again without
counting the cells.
4. Add 1-2.5 mL of the prepared 1× Extraction
Buffer A per 2-5 × 107 cells. Incubate on ice for
10-15 minutes.
5. Homogenize
Examination of Non-Specifically Bound Proteins and Peptides Following Depletion of Twenty High Abundance Proteins from Human Plasma
4009 30 515 13 3860 31 472 13 3887 33 767 20 1357 22 4445 30 1919 31
IPI00022429 a-1-acid glycoprotein 1 precursor 103 4 118 4 94 4 220 5 86 3 168 6 107 3 181 5
Post-Translational Modifications
142
Y142P
H2B
1-19
unmod
H2B
1-19
K5ac
10 ng
C
H2B
1-19
K5me1
H2B
1-19
K12ac
H2B
1-19
S14P
H2B
1-19
K15ac
H2B
107-125
Product Information Sheet-11667327001
.0, is recommended as a starting point.
Average yields range from 75–300 µg / 1.1 - 4.2 × 107 cells
1
2
3
4
Data Sheet - P9247
4. Khakh, B.S., et al., Pharmacol Rev,. 53, 107
(2001).
5. Ding, Y., et al., J. Auton. Nerv. Syst,. 81, 289
(2000).
6. Le, K.T., et al., J. Neurosci., 18, 7152 (1998).
7. Robertson, S.J., et
Data Sheet - P4746
4. Khakh, B.S., et al., Pharmacol Rev,. 53, 107
(2001).
5. Ding, Y., et al., J. Auton. Nerv. Syst,. 81, 289
(2000).
6. Le, K.T., et al., J. Neurosci., 18, 7152 (1998).
7. Robertson
Data Sheet - P8997
4. Khakh, B.S. et al., Pharmacol. Rev., 53, 107
(2001).
5. Ding, Y. et al., J. Auton. Nerv. Syst., 81, 289
(2000).
6. Le, K.T. et al., J. Neurosci., 18, 7152 (1998).
7. Robertson
QCM™ Endothelial Cell Migration Assay
A+
La
t
FB
S+
La
t
BS
A+
CD
FB
S+
CD
0
1.0×107
2.0×107
3.0×107
4.0×107
5.0×107
6.0×107
7.0×107
8.0×107
9.0×107
1.0×108
1.1×108
1.2×108
1.3×108
1.4×108
BSA
FBS
BSA+Lat
FBS+Lat
Product Information Sheet - PKH2GL
stain at final concentrations of 2 x 10-6 M PKH2 dye
and 1 x 107 cells/ml in a 2 ml total volume, perform the
followingusing aseptic techniques:
1. Adherent or bound cells must first be removed
using
EZ- Magna ChIRP RNA Interactome Kit Analysis and Mapping of Chromatin: Noncoding RNA Interactions
cold PBS.
13. Pellet the Cells by Centrifuging at 2000 x g for 5 minutes at 4º C.
14. Aspirate supernatant carefully .
15. Resuspend the cell pellet with cold PBS. (1.0 ml per 1 x 107
Magna ChIRP¿ RNA Interactome Kits Analysis and Mapping of Chromatin: Noncoding RNA Interactions
cold PBS.
13. Pellet the Cells by Centrifuging at 2000 x g for 5 minutes at 4º C.
14. Aspirate supernatant carefully .
15. Resuspend the cell pellet with cold PBS. (1.0 ml per 1 x 107
Quality Control Ranges - Human MMP Magnetic Bead Panel 1
RANGES
MILLIPLEX® MAP Human MMP Magnetic Bead Panel 1
Kit Catalog # HMMP1MAG-55K
Control Catalog # HMMP1MG-6055-1
Lot# HMMPM-107 and HMMPM-207
http://www.emdmillipore.com/
EZ-ChIP¿ Chromatin Immunoprecipitation Kit
pellet cells.
13. During spin, prepare lysis buffer by adding 5 µL of Protease Inhibitor Cocktail II to each 1 mL of SDS Lysis Buffer
required.
• For every 1 x 107 HeLa cells
Quality Control Range Sheet- MCY-112 and MCY-212
Control 1 107 – 222 pg/mL IL-15 Control 1 100 – 208 pg/mL
Control 2 586 – 1218 pg/mL Control 2 516 – 1071 pg/mL
Data Sheet
Repeat the Wash Step once again without
counting the cells.
7. Add 1-2.5 mL of the prepared 1× Extraction
Buffer A per 2-5 × 107 cells. Incubate on ice for
10-15 minutes.
8. Homogenize
Quality Control Range Sheet- MCY-112 and MCY2-212
Control 1 107 – 222 pg/mL IL-15 Control 1 100 – 208 pg/mL
Control 2 586 – 1218 pg/mL Control 2 516 – 1071 pg/mL
QCM™ Endothelial Cell Invasion Assay (24 well, fluorometric)
errors.
control 2% FBS CalceinAM only
0
5.0×106
1.0×107
1.5×107
2.0×107
2.5×107
3.0×107
3.5×107
Fl
uo
re
sc
en
ce
in
te
ns
ity
(c
ou
nt
s/
se
c)
EX-CELL 420 Serum Medium for the Growth of Spodopteran (Sf9 and Sf21) Insect Cells
References
1. Vaughn, J.L., Goodwin, R.H., Tompkins, G.J., and McCawley,
P. (1977). The establishment of two cell lines from the insect
Spodoptera frugiperda (Lepidoptera: Noctuidae). In Vitro 13,
213-
Quality Control Ranges: Milliplex Human Kidney Injury Magnetic Bead Panel 2
Control 2 42 - 87 ng/mL
α1-Microglobulin Control 1 6.2 - 13 ng/mL
Control 2 64 - 132 ng/mL
Data Sheet - N3398
B.A., Methods in
Enzymology, 269, 385, (1996).
12. Ku, D.D., Methods in Enzymology, 269, 107,
(1996).
13. Okishio, M. et al., Biochem. Biophys. Res.
Commun. 183, 849, (1992).
14. Catani, M.V.
Data Sheet - S0439
References
1. Hickey, C.M. et al., Nat. Rev. Mol. Cell Biol.,
13(12), 755-766 (2012).
2. Pichler, A. et al., Biomol. Concepts, 8(1), 13-36
(2017).
3. Mahajan, R. et al., Cell, 88(1), 97-107 (1997)
Product Information Sheet - GDI3
Small or valuable (1 to 100 :l or 1 mg) Procedure #1
Moderate
(3 to 100 mg tissue, 103-107 mammalian cells or 109 bacterial cells)
Procedure #2
Large (0.1 to 1.0 g tissue or 107-108 mammalian cells
Quality Control Range Sheet- HKI1-107 and HKI1-207
Panel 1
Catalog # HKI1MAG-99K
Control Catalog # HKI1-6099-1 Lot # HKI1-107 and HKI1-207
CAT# HKI1-6099-1, Lot# HKI1-107.207 (27 November 2023)
EMD MILIPORE • 1-800-645-5476 • 1-800-645
Data Sheet - E107
ultimate disposition of the material.
References:
1. Martin, B.R., Lue, L.P., Boni, J.P. "Pyrolysis and volatilization of cocaine." J. Anal. Toxicol. 13, 158-62
(1989).
2. Schultz, O.E., Al-Yahya
Product Information Sheet - G8772
(25-300 °C): 85 x 107 per °C
Strain point: 505 °C
Annealing point: 548 °C
Dielectric Constant: 1 Kc 7.6 D.C.
Volume Resistivity (Ohm-cm): 25 °C 6.5 x 1012
Lost tangent: 1 Kc 2.0% 100 Kc
Data Sheet - N6412
References
1. Miki, B., and McHugh, S., J. Biotechnol., 107, 193-
232 (2004).
2. Beck, E., et al., Gene, 19, 327-336 (1982).
3. Mazodier, P., et al., Nucl. Acids Res., 13, 195-205
(1985
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