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The Effective Use of Protein Kinase Inhibitors
78 38 65 34 91 93 96 66 39 93 79 94 103
69
109 20 105 86 90 3 3 96 89 71 93 76 94 96
7 2 18 22 5 75 68 84 54
The Effective Use of Protein Kinase Inhibitors
78 38 65 34 91 93 96 66 39 93 79 94 103
69
109 20 105 86 90 3 3 96 89 71 93 76 94 96
7 2 18 22 5 75 68 84 54
Quality Control Ranges: MILLIPLEX® MAP Mouse Cytokine/Chemokine Panel
Cytokine QC Level Expected Range Units
Eotaxin Control 1 94 - 196 pg/mL IL-6 Control 1 97 - 201 pg/mL LIX Control 1 109 - 226 pg/mL
Control 2 550 - 1143 pg/mL
Data Sheet - SRP5063
Reagent. Shake the
96-well plate and then incubate the reaction mixture
for another 40 minutes at ambient temperature.
6. Then add 50 µl of the Kinase Detection Reagent to
the 96-well plate and incubate
Suspension MDCK Cell Culture in EX-CELL MDCK Serum-Free Medium
tested. Both infection
conditions produced peak TCID50 titers in the range of
108 - 109 particles/mL between 72 - 96 hours post-infection
(Figure 4).
Summary
EX-CELLTM MDCK is a serum-free medium developed
Quality Control Range Sheet- FCY-109 and FCY-209
Panel
Catalog # FCYTMAG-20K-PMX and FCYTMAG20KPX19BK
Control Catalog # FCY-6020 Lot # FCY-109 and FCY-209
Note: The Quality Control Ranges are generated using serum matrix provided in the kit
Cell Culture Poster
cells® as
above. After three days of growth at 37°C and 5% CO2, the spinners
were infected with 3 x 109 virus particles (moiª3) of the rAd5 containing
the ß-galactosidase (ß-gal) reporter gene (Crucell
Product Information Sheet - P5778
3 of 3
09/03/96 - TMG
PHOSPHATASE, ALKALINE
from Bovine Intestinal Mucosa
Sigma Prod. No. P5778
REFERENCES:
1. Neumann, H. and Lustig, A., European Journal of Biochemistry, 109, 475-480 (1980
Quality Control Range Sheet- MAGP-109 and MAGP-209
Growth Factor Magnetic Bead Panel
Catalog# MAGPMAG-24K
Control Catalog # MAGP-6024 LOT# MAGP-109 and MAGP-209
Additional Analytes on Reverse Side
Note: The Quality Control Ranges are generated
Quality Control Range Sheet- A60MG-104 and A60MG-204
Control 2 109 - 227 pg/mL Control 2 92 - 192 pg/ml
IL-5 Control 1 25 – 52 pg/mL IL-22 Control1 503 – 1045 pg/ml
Control 2
User Guide-RAB0016
Serum 85 82-109
Plasma 82 95-116
Cell Culture Supernatants 97 76-92
Linearity
Sample Type Serum Plasma Cell Culture Supernatants
1:2
Average % of Expected 110 109 109
Range (%) 99-
Product Information Sheet - CKH200A
105
96-112
102
97-107
105
100-113
100
87-109
106
91-115
Average % of Expected 1:8
Range (%)
107
94-121
100
96-109
102
93-107
MILLIPLEX®MAP Human Myokine Panel is an optimized quantitative immunoassay that simultaneously measures 15 novel muscle-secreted factors
FABP3 Irisin FSTL1 OSM IL-6 FGF21 Musclin
Average
+std 3 100 96 105 71 90 95 109 95 97 105 119 84 100 82 106
+std 4 92 104 113 77 90 101 105 128 93 115
Product Information Sheet - L6662
Deprenyl hydrochloride
M0763 Metoclopramide hydrochloride
291552 6-Methoxy-1,2,3,4-tetrahydro-9H-pyrido[3,4b]
indole
M-109 2-Methyl-5-hydroxytryptamine maleate
M-110 alpha-Methyl-5-hydroxytryptamine
User Guide-RAB0013
Plasma Cell Culture Supernatants
1:2
Average % of Expected 97 102 105
Range (%) 89-105 94-109 96-109
1:4
Average % of Expected Range 98 76 93
Range (%) 87-106 67-82 83-99
4
RAB0013pis Rev
Data Sheet - P2110
Pierce, g., et al., J. Cell. Biol., 109, 429 (1989).
4. Ross, R., Arteriosclerosis, 1, 293, (1981).
5. Heldin, C.H., and Westermark, B., Physiol. Rev.,
79, 1283 (1999).
6. Heldin, C.H., et al., Biochem.
User Guide-RAB0543
The
kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Components
Human IL-18 Microplate - RAB0543A-1EA-KC: 96 wells (12 strips x 8 wells) coated with
Product Information Sheet - F4428
et al., Drug Resist. Updat., 4, 233-242
(2001).
6. Budd, R.C., J. Clin. Invest., 109, 437-442 (2002).
7. Hohlbaum, A.M., Clin. Immunol., 103, 1-6 (2002).
Related Products
Monoclonal Anti-Fas Ligand
Poster: Improved Method For Adeno and Lenti- Virus Purification and Titration
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104 105 106 107 108 109 1010
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Poster: Improved Method for Adeno and Lenti-Virus Purification and Titration
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1
Ammonium bicarbonate (A6141)
Mater. Res., 61(1),
109-120 (2002).
3. Navarro, J. M., et al., Ammonium, bicarbonate and
calcium effects on tomato plants grown under
saline conditions. Plant Sci., 157(1), 89-96 (2000).
4. Kuberan
User Guide-RAB1437
The
kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Components
Human Eph A1 Microplate - RAB1437A-1EA: 96 wells (12 strips x 8 wells) coated with
Pharmacological Profiling in Human Embryonic Progenitor Cells Using High Content Analysis
non-engineered cells. To evaluate this we
performed large-scale pharmacological profiling, testing 109 GPCR ligands (capable of
activating >150 different receptors) in six hES-derived progenitor cell lines
Product Information Sheet - MAK331
4. Carefully transfer 24 L of supernatant into a clean
tube.
5. Add 96 L of Assay Buffer. Label this tube “HDL”.
6. Carefully remove all remaining supernatant from
the pellet.
7. Transfer
Data Sheet - 70137
References:
1. S. Endo, Über ein Verfahren zum Nachweis von Typhusbacillen, Centralbl. Bakt. I. Orig., 35, 109
(1904)
2. G. Naundorf, N.G. Aumen, The assessment of ammonia-induced cell envelope injury in E
Quality Control Ranges: MILLIPLEX® MAP Human Cytokine/ Chemokine Magnetic Bead Panel
Control 2 523 - 1086 pg/mL Control 2 465 - 966 pg/mL
IL-6 Control 1 100 - 207 pg/mL *RANTES Control 1 96 -
MultiScreen® Permeability Filter Plate Assembly User Guide
noncell-based permeability assays. Journal of the
Association for Laboratory Automation 12(2):104–109.
http://journals.sagepub.com/doi/pdf/10.1016/
j.jala.2006.10.008
3. Wohnsland, F. and Faller
User Guide-RAB0097
. The
kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Components
Rat CRP Microplate - RAB0097A-EA: 96 wells (12 strips x 8 wells) coated with anti-Rat
Data Sheet - K4761
contains the
receptor-binding domain and a potential N-linked
glycosylation site at amino acid residue 109.
Human TRAIL shares about 65% amino acid sequence
homology with mouse TRAIL and is active on mouse
Product Information Sheet - A5394
Chin A., Analytical Biochem., 193, 16 (1991).
9. Solheim, L.P. and Fromm H.J., Analytical Biochem., 109, 266 (1980).
10. McCarty, R.E., Analytical Biochem., 205, 371 (1992).
11. Seidel, J.C. and Gergely
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