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Analysis of 20 Underivatized Amino Acids

Discover an innovative LC-MS/MS method for amino acid analysis using the Supel™ Carbon LC column with excellent retention and fast run times.

MilliporeSigma Invests $20 Million in New Switzerland Laboratory

MilliporeSigma has announced plans to build a new, $20 million laboratory facility in Buchs, Switzerland to support its rapidly growing reference materials business.

Column Packing & Separation with Sephadex® LH-20

Separation using Sephadex LH-20 column for Size Exclusion Chromatography media in polysaccharide network from Cytiva.

Enzymatic Activity of Glucose-6-Phosphatase [EC 3.1.3.9]

To measure glucose-6-phosphatase activity, the Taussky-Shorr method is used. This method is a spectrophotometric stop-rate determination assay that is measured at 660 nm.

Oligonucleotide Standard 6 Mix LC-UV Analysis

Chromolith® RP-18e columns optimize Oligo Standard 6 separation with varied flow rates and ion-pairing reagent evaluation.

Ni Sepharose 6 Fast Flow for Protein Purification

Ni Sepharose 6 Fast Flow purifies histidine-tagged proteins efficiently, offering high cross-linked agarose beads with Ni2+ ions.

Performing a Separation with IgG Sepharose 6 Fast Flow

Perform a separation with IgG Sepharose 6 Fast Flow from Cytiva, an Affinity Chromatography product for purification of recombinant fusion proteins containing a protein A tail.

Enzymatic Assay of Glucose-6-Phosphate Dehydrogenase (EC 1.1.1.49)

To measure glucose-6-phosphate dehydrogenase activity, beta-nicotinamide adenine dinucleotide phosphate is used in a spectrophotometric rate determination assay at 340 nm.

Calcium Phosphate Transfection Kit Protocol

Calcium phosphate transfection is a common method for the introduction of DNA into eukaryotic cells. This protocol can be optimized for use with a wide variety of cell types.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

Sample Preparation for Chromatographic Purification

This page discusses various aspects of sample preparation for chromatographic purification.

Whole Transcriptome Amplification of RNA from Low Cell-Number Samples

The efficacy of amplification of small quantities of total RNA with the Complete Whole Transcriptome Amplification Kit (WTA2) was examined in this study.

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

Chemical Synthesis of Monodisperse Magnetic Nanoparticles

Magnetic nanoparticles have attracted tremendous attention due to their novel properties and their potential applications in magnetic recording, magnetic energy storage and biomedicine.

Dextran

Dextran polymer details: composed mainly of alpha-D-(1-6) linkages with varied branch lengths.

Quantum Dots: An Emerging Class of Soluble Optical Nanomaterials

We presents an article concering Quantum Dots: An Emerging Class of Soluble Optical Nanomaterials.

Performing a Separation or Removal of Albumin with HiTrap® Blue HP and Blue Sepharose 6 Fast Flow

This page shows how to separate or remove albumin by affinity chromatography using HiTrap Blue HP and Blue Sepharose 6 Fast Flow.

Summer Sale Promo - Protein Prep & Reagents

Now through July 29, save up to 20% off select protein prep and reagent products

Analytical Method: COD Measurement in Waste Water (Rapid-Digestion Method)

Analytical Method COD measurement in wastewater - Photometric determination of chromate consumption subsequent to digestion in 20 min.

Enzymatic Assay of Peroxidase (EC 1.11.1.7) 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a Substrate

To standardize a procedure for the assay of Peroxidase using 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a substrate.

Sorting Carbon Nanotubes and Their Biological Applications

Carbon nanotubes (CNTs) have received much attention since their discovery in 1991 by Sumio lijima1 due to their excellent mechanical, electrical, and optical properties.

Materials Design Concepts for Efficient Blue OLEDs: A Joint Theoretical and Experimental Study

Isotopic Labeling for NMR Spectroscopy of Biological Solids

Isotopic Labeling for NMR Spectroscopy of Biological Solids;

Reflectometric Determination of Nitrite in Meat Products

Reflectometric protocol enabling rapid quantification of nitrite in meat product samples with RQflex® 20 reflectometer.

Media Sterilization – Plant Tissue Culture Protocol

Plant tissue culture media are generally sterilized by autoclaving at 121 °C and 1.05 kg/cm2 (15-20 psi). The time required for sterilization depends upon the volume of medium in the vessel.

Mesoporous Silica Applications

Mesoporous silica used in wastewater remediation, air cleaning, catalysis, bio-catalysis, and drug delivery.

Organosilanols

Pd-catalyzed cross-coupling of silicon compounds offers an alternative in synthetic chemistry.

Standard Methods 6040D: GC Analysis of Geosmin and 2-MIB on SLB®-5ms after SPME using 50/30 µm DVB/Carboxen/PDMS Fiber

Separation of 2-Isopropyl-3-methoxypyrazine 20 ppt; 2-Isobutyl-3-methoxypyrazine; 2-Methylisoborneol; (±)-Geosmin 10 ppt

TBST for Western Blotting

Tris-buffered saline with Tween® 20 Detergent is an effective wash buffer for immunoassays like protein purification and Western blot.

Group 11 Thin Films by Atomic Layer Deposition

Copper metal deposition processes are an essential tool for depositing interconnects used in microelectronic applications, giving group 11 (coinage metals: Copper, Silver, and Gold) an important place in atomic layer deposition (ALD) process development.

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