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174148-03-9

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Showing 1-30 of 488 results for "174148-03-9" within Site Content

X-tremeGENE™ 9 DNA Transfection Reagent Protocol

Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).

CRISPR Cas 9 Nuclease RNA-guided Genome Editing

Learn about CRISPR Cas9, what it is and how it works. CRISPR is a new, affordable genome editing tool enabling access to genome editing for all.

MilliporeSigma Expands in Gillingham, UK, Boosts Distribution Capabilities

MilliporeSigma today announced the completion of a £9 million expansion to its Gillingham distribution center.

How to Boost AAV Titer with Feed Supplementation in both HEK293 and Sf-9 Platforms

In this webinar, you will learn wow to boost AAV titers in HEK293 and Sf-9 cell lines as well as getting some insider tips on volume and timing optimization.

Accelerated mAb Development Program – From Transfection to GMP Drug Substance in 9 Months

The accelerated mAb development program delivers GMP drug substance nine months from transfection. We designed the mAbExpress™ program to be fast, cost effective, and appropriate for the early clinical phase of your program, with regulatory support throughout.

Analysis of 17 Cannabinoids in Hemp and Cannabis

HPLC separation of 17 important cannabinoids including CBD, delta 9 THC and THCA. Read the application note.

Determination of Phosphorus Total in Meat and Meat Products

Colorimetric total phosphorus analysis method for meat and meat products as per German Food and Feed Code §64 LFGB 06.00-9.

Solving Aspartimide Formation in Fmoc SPPS with Fmoc-Asp(OBno)-OH

Aspartimide formation 1,2 is caused by repeated exposure of aspartic acid-containing sequences to bases like piperidine and can result ultimately in the generation of 9 different by-products.

Analysis of Antioxidants in Bakery Products

HPLC-UV method detects 9 antioxidants like propyl gallate in food using a Discovery®</sup HS C18 column for excellent peak shapes.

Performing a Separation with HiTrap® Protein G HP

Purify monoclonal or polyclonal IgG from serum, cell culture supernatant or ascitic ﬂuid using the HiTrap Protein G HP from Cytiva, an affinity-exclusion chromatography product containing Sepharose-immobilized Protein G.

Revolution in Plastic Recycling: Laser Marking & Digital Product Passports Enable True Circular Economy

An industry-wide project demonstrates how packaging can become smarter, more sustainable, and fully traceable. Plastics are a blessing—and a global problem. Lightweight, durable, and versatile. Yet these very properties make plastics a challenge worldwide. Over 400 million tons are produced

GC Analysis of PBDE Flame-Retardant Compounds

Applying Equilibrated Immobiline® DryStrip Gels to SDS Gels

This page shows how to apply equilibrated Immobiline DryStrip gels

Whole Transcriptome Amplification of RNA from Low Cell-Number Samples

The efficacy of amplification of small quantities of total RNA with the Complete Whole Transcriptome Amplification Kit (WTA2) was examined in this study.

Performing a Purification of IgM Antibodies with HiTrap™ IgM Purification HP

This page shows how to purify IgM antibodies by afﬁnity chromatography using HiTrap™ IgM Purification HP.

Immunology 2025

Over 3,500 scientists are expected to attend IMMUNOLOGY2025™ to hear about cutting-edge science and learn of innovations and products that can benefit their research programs.

SMCxPRO® Immunoassay Platform

Explore the affordable SMCxPRO® immunoassay platform for ultrasensitive biomarker detection. See how you can reliably detect low-level proteins down to the femtogram/mL.

General Instructions for Affinity Purification Using HiTrap® Columns

General instructions for affinity purification using HiTrap columns from Cytiva.

Degradable Poly(ethylene glycol) Hydrogels for 2D and 3D Cell Culture

Progress in biotechnology fields such as tissue engineering and drug delivery is accompanied by an increasing demand for diverse functional biomaterials. One class of biomaterials that has been the subject of intense research interest is hydrogels, because they closely mimic

IgG Antibody Purification with HiTrap® Protein G HP Columns

This page shows how to separate IgG antibodies by afﬁnity chromatography using HiTrap® Protein G HP columns from Cytiva.

Gravity-ﬂow Puriﬁcation using His GraviTrap™ and His GraviTrap™ Kit

This page shows how to purify histidine-tagged recombinant proteins by gravity flow using His GraviTrap and His GraviTrap Kit from Cytiva.

3D Neurosphere Culture Media

3D neurosphere culture protocol to expand human U-87 MG glioblastoma cells in serum-free/xeno-free stem cell culture media. Explore over 350 PromoCell products on Sigma.com.

How to Automate the SMC® Immunoassay Workflow

Reduce sources of error and variability in sensitive methods to quantify biomarkers by automating the SMC® immunoassay workflow with the Hamilton Microlab® STARlet liquid handling workstation.

Spermine Tetrahydrochloride for Molecular Biology

This product is suitable for DNA precipitation from low salt aqueous buffers. It has been tested for the absence of proteases and nucleases.

Face Care Formulation Examples

Targeted face care nowadays is all about high performance and well-being. Our portfolio of various skin care active ingredients offers you a treasure you can use to fulfill all desires to improve skin conditions. One can start from immediate impact

Cultrex ® 3-D Culture Matrix™ Laminin I Protocol

3-D Culture Matrix™ Mouse Laminin I may be used as a gel or as a media additive to study cellular growth and differentiation in three dimensions in vitro.

Conducting Polymer Device Applications

The application of conducting polymers at the interface with biology is an exciting new trend in organic electronics research.

Healthy Glowing Skin Secret

While overall trends in skincare products are constantly changing, the desire for healthy, glowing skin remains timeless across all ethnicities and age groups. Various approaches to achieving radiant skin, such as glass skin, dolphin skin, mirror skin, dewy skin, or

Purification of Histidine-Tagged Proteins using HiTrap® IMAC HP and HiTrap® IMAC FF

This page shows how to purify histidine-tagged proteins using HiTrap® IMAC HP and HiTrap® IMAC FF from Cytiva.

The following procedure has been evaluated with Ficoll-Paque PLUS and is recommended for separation of normal blood samples for maximum reproducibility.

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