181978

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Showing 1-6 of 6 results for "181978" within Papers

Recombinant N-glycosylation isoforms of Legume lectins: Production and purification from Nicotiana benthamiana leaves following RuBisCO depletion.

Kevin Bellande et al.

Plant physiology and biochemistry : PPB, 157, 441-452 (2020-11-20)

An efficient purification of recombinant proteins often requires a high ratio of recombinant to host proteins. In plants, Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) is the most abundant leaf protein, thus strongly impacting purification yield. Here, we describe a simple and robust purification

Polyelectrolyte precipitation of beta-galactosidase fusions containing poly-aspartic acid tails.

J Y Zhao et al.

Journal of biotechnology, 14(3-4), 273-283 (1990-06-01)

Protein recovery from industrial microbial processes can be very expensive, often exceeding the cost of protein production. We have genetically engineered 3 beta-galactosidase (beta-gal) fusion proteins containing poly-aspartic acid tails to test the effect of the tails on recovery by

Versatile live-cell activity analysis platform for characterization of neuronal dynamics at single-cell and network level.

Xinyue Yuan et al.

Nature communications, 11(1), 4854-4854 (2020-09-27)

Chronic imaging of neuronal networks in vitro has provided fundamental insights into mechanisms underlying neuronal function. Current labeling and optical imaging methods, however, cannot be used for continuous and long-term recordings of the dynamics and evolution of neuronal networks, as

Continuous, quantifiable, and simple osmotic preconcentration and sensing within microfluidic devices.

Andrew Jajack et al.

PloS one, 14(1), e0210286-e0210286 (2019-01-17)

Insurmountable detection challenges will impede the development of many of the next-generation of lab-on-a-chip devices (e.g., point-of-care and real-time health monitors). Here we present the first membrane-based, microfluidic sample preconcentration method that is continuous, quantifiable, simple, and capable of working

ESCargo: a regulatable fluorescent secretory cargo for diverse model organisms.

Jason C Casler et al.

Molecular biology of the cell, 31(26), 2892-2903 (2020-10-29)

Membrane traffic can be studied by imaging a cargo protein as it transits the secretory pathway. The best tools for this purpose initially block export of the secretory cargo from the endoplasmic reticulum (ER) and then release the block to

Loss of INPP4B causes a DNA repair defect through loss of BRCA1, ATM and ATR and can be targeted with PARP inhibitor treatment.

Laura R H Ip et al.

Oncotarget, 6(12), 10548-10562 (2015-04-15)

Treatment options for ovarian cancer patients remain limited and overall survival is less than 50% despite recent clinical advances. The lipid phosphatase inositol polyphosphate 4-phosphatase type II (INPP4B) has been described as a tumor suppressor in the PI3K/Akt pathway with

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