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Showing 1-26 of 26 results for "198781" within Papers
P Jezek et al.
FEBS letters, 408(2), 166-170 (1997-05-19)
Fatty acid (FA) uniport via mitochondrial uncoupling protein (UcP) was detected fluorometrically with PBFI, potassium-binding benzofuran phthalate and SPQ, 6-methoxy-N-(3-sulfopropyl)-quinolinium, indicating K+ and H+, respectively. The FA structural patterns required for FA flip-flop, UcP-mediated FA uniport, activation of UcP-mediated H+
D D Giera et al.
Fundamental and applied toxicology : official journal of the Society of Toxicology, 16(2), 348-355 (1991-02-01)
Assessment of hepatic omega-oxidation of fatty acids by cytochrome P450IV enzymes in toxicology studies can be a means of evaluating test compound effects on peroxisomal proliferation. Routine assay of omega-oxidation, however, requires a simpler method of enzymatic analysis than currently
Valérie Clermont et al.
British journal of clinical pharmacology, 85(6), 1367-1377 (2019-03-01)
To characterize mRNA expression levels (17 cytochromes P450) and activity (9 isoforms) of major cytochromes P450 expressed throughout the human small intestine. Tissue samples were obtained from 9 deceased subjects and intestinal sections (n = 10) were isolated for each subject. Relative
Paresh C Sanghani et al.
Biochemistry, 41(35), 10778-10786 (2002-08-28)
The human glutathione-dependent formaldehyde dehydrogenase is unique among the structurally studied members of the alcohol dehydrogenase family in that it follows a random bi bi kinetic mechanism. The structures of an apo form of the enzyme, a binary complex with
P C Sanghani et al.
Biochemistry, 39(35), 10720-10729 (2000-09-09)
Formaldehyde, a major industrial chemical, is classified as a carcinogen because of its high reactivity with DNA. It is inactivated by oxidative metabolism to formate in humans by glutathione-dependent formaldehyde dehydrogenase. This NAD(+)-dependent enzyme belongs to the family of zinc-dependent
D E Jensen et al.
The Biochemical journal, 331 ( Pt 2), 659-668 (1998-06-11)
An enzyme isolated from rat liver cytosol (native molecular mass 78. 3 kDa; polypeptide molecular mass 42.5 kDa) is capable of catalysing the NADH/NADPH-dependent degradation of S-nitrosoglutathione (GSNO). The activity utilizes 1 mol of coenzyme per mol of GSNO processed.
H A Dirven et al.
Journal of chromatography, 564(1), 266-271 (1991-03-08)
The formation of omega-hydroxylauric acid from lauric acid is an indicator of the activity of cytochrome P-450 IV family proteins. The two main metabolites of lauric acid, (omega-1)-and omega-hydroxylauric acid, have been completely separated by reversed-phase high-performance liquid chromatography. Measurement
Jade Huguet et al.
Xenobiotica; the fate of foreign compounds in biological systems, 49(2), 187-199 (2018-02-17)
1. The objective of our study was to develop and validate a cocktail approach to allow the simultaneous characterization of various CYP450-mediated oxidations by human heart microsomes for nine probe drug substrates, namely, 7-ethoxyresorufin, bupropion, repaglinide, tolbutamide, bufuralol, chlorzoxazone, ebastine
Y Gotoh et al.
Biochimica et biophysica acta, 1043(1), 52-56 (1990-03-12)
When 20-hydroxyleukotriene B4 (20-OH-LTB4) is incubated at pH 10.5 in the presence of NAD+ with an alcohol dehydrogenase isolated from human neutrophils, a polar product is formed as detected on reverse-phase high-performance liquid chromatography (RP-HPLC). The product is identified as
M s Shet et al.
Archives of biochemistry and biophysics, 330(1), 199-208 (1996-06-01)
The recombinant fusion protein rF450[mRat4Al/mRatOR]L1, containing the heme domain of P450 4A1 and the flavin domains of NADPH-P450 reductase, when incubated with dilaurylphosphatidylcholine (DLPC), Chaps, cytochrome b5, and a 20-fold excess of purified NADPH-P450 reductase, catalyzes the omega- oxidation of
Y H Yang et al.
Archives of biochemistry and biophysics, 352(2), 271-280 (1998-05-20)
A cDNA clone was isolated from a female rainbow trout liver lambda g tau 11 library using polyclonal antibodies raised against rainbow trout cytochrome P450 LMC1. This 2149-nucleotide clone contained an open reading frame encoding a protein of 499 amino
Karen M J Saerens et al.
FEMS yeast research, 15(7) (2015-08-25)
Altering glycolipid structure by genetic engineering of Starmerella bombicola is a recently started research topic and worthy alternative to the unsuccessful selective feeding strategies conventionally applied to reach this goal. One question to be addressed when expressing heterologous proteins in
Hiroki Ebata et al.
Macromolecular bioscience, 8(1), 38-45 (2007-10-24)
Novel green and sustainable elastomers having both good biodegradability and chemical recyclability properties were designed and synthesized using potentially biobased materials and lipase as an environmentally benign catalyst. High molecular weight poly[(12-hydroxydodecanoate)-co-(12-hydroxystearate)] [poly(12HD-co-12HS)] samples with varying monomer ratios were prepared
Polymerized phosphatidyl choline vesicles. Stabilized and controllable time-release carriers.
S L Regen et al.
Biochemical and biophysical research communications, 101(1), 131-136 (1981-07-16)
Inge Van Bogaert et al.
Biotechnology and bioengineering, 108(4), 734-741 (2011-03-16)
The naturally occurring sophorolipids synthesized by Candida bombicola possess--despite their overall heterogeneity--little variation in the length of the lipid tail. The range is limited to C16-C18 fatty acids and is governed by the specificity of a cytochrome P450 monooxygenase. However
Yeon Jung Choi et al.
Archives of toxicology, 92(11), 3325-3336 (2018-09-28)
Cytochrome P450 (CYP) enzymes, particularly CYP4A/4F, are the major ω-hydroxylases of arachidonic acid (AA) that can produce 20-hydroxyeicosatetraenoic acid (20-HETE). Although there are dissimilarities in substrate specificity, tissue distribution, and gene regulation between CYP4A and CYP4F, selective CYP4A or 4F
S Svensson et al.
Journal of molecular biology, 302(2), 441-453 (2000-09-06)
The structure of mouse class II alcohol dehydrogenase (ADH2) has been determined in a binary complex with the coenzyme NADH and in a ternary complex with both NADH and the inhibitor N-cyclohexylformamide to 2.2 A and 2.1 A resolution, respectively.
Y Amet et al.
Journal of chromatography. B, Biomedical applications, 681(2), 233-239 (1996-06-07)
The formation of (omega-1)-hydroxylauric acid from lauric acid (LA) can be used as an indicator of the activity of cytochrome P450 2E1 (CYP2E1) in rat and human liver microsomes. A high-performance liquid chromatographic (HPLC) method that is capable of identifying
Paresh C Sanghani et al.
Biochemistry, 45(15), 4819-4830 (2006-04-12)
The active-site zinc in human glutathione-dependent formaldehyde dehydrogenase (FDH) undergoes coenzyme-induced displacement and transient coordination to a highly conserved glutamate residue (Glu-67) during the catalytic cycle. The role of this transient coordination of the active-site zinc to Glu-67 in the
Elena Bailo et al.
Analytical and bioanalytical chemistry, 394(7), 1797-1801 (2009-06-16)
Surface-enhanced Raman scattering was used as a spectroscopic tool to investigate the changes brought upon cytochrome P450BSss after fatty acid binding. Differences in the spectra of substrate-free and substrate-bound enzyme were observed indicating the potential for this method to be
Alexander N Grechkin et al.
FEBS letters, 549(1-3), 31-34 (2003-08-14)
Guava (Psidium guajava) hydroperoxide lyase (HPL) preparations were incubated with [1-(14)C](9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid for 1 min at 0 degrees C, followed by rapid extraction/trimethylsilylation. Analysis of the trimethylsilylated products by gas chromatography-mass spectrometry and radio-high-performance liquid chromatography revealed a single predominant
Miwa Kondo et al.
Toxicological sciences : an official journal of the Society of Toxicology, 168(2), 572-596 (2019-01-11)
The nongenotoxic pyrethroid insecticide permethrin produced hepatocellular tumors in CD-1 mice but not in Wistar rats. Recently, based on findings of a Pathology Working Group involving an expert panel of pathologists, it was concluded that permethrin increased liver tumors at
Myeongjin Yi et al.
Toxicology and applied pharmacology, 330, 100-106 (2017-07-22)
Because macrophages respond to a variety of pathological and pharmacological reagents, understanding the role of P450s in macrophages is important for therapeutic intervention. There has been a lack of research on CYP4 in macrophages, but fatty acid accumulation and lipid
Junjun Wang et al.
International journal of molecular sciences, 19(3) (2018-03-15)
This study was aimed to clarify the effect of honokiol (Hon) on the activity of Cytochrome P450 (CYP450) enzymes, and the level of mRNA expression of liver and kidney transporters in type 2 diabetic rats induced by high-fat diet and
J J Hedberg et al.
FEBS letters, 436(1), 67-70 (1998-10-15)
Human class I alcohol dehydrogenase was mutated at positions 57 and 115, exchanging for Asp and Arg respectively, in an attempt to introduce glutathione-dependent formaldehyde dehydrogenase characteristics. In addition, class III alcohol dehydrogenase, identical to glutathione-dependent formaldehyde dehydrogenase, was mutated
Yazun Bashir Jarrar et al.
Annals of human genetics, 84(5), 400-411 (2020-05-13)
The objectives of the present study were to identify CYP4V2 genetic variants and characterize their functional consequences. A total of 26CYP4V2 genetic variants were identified, including seven novel variants in 60 randomly selected healthy subjects. Six protein-coding variants were studied
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