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MF-Millipore Filters: A biologically inert mixture of cellulose acetate and cellulose nitrate
(500/pk)
SWP 055 00 HAWP 050 00 DAWP 050 00 RAWP 050 00 SSWP 050 00 SMWP 070 00 SCWP 050 00
(55 mm) (50 mm) (50 mm) (50 mm) (50 mm) (70 mm; 25/pk) (50 mm)
Estapor®Microspheres for Lateral Flow
030 Black • 0.270 -0.330 200 - 500 80380143
K1-050 Black • 0.450 -0.530 1 - 10 80380238
K-030 Blue • 0.270 -0.325 None 39428080
K1-030 Blue • 0.270 -0.330 200 - 500 80380065
K1-050 Blue • 0.450 -0.530 1 -
NovAseptic Valve - Valve Body, Flow through 180˚, TC Connectable
Specifications subject to change without notice.
Lit. No. SP1107EN00 Rev. -, 09/2006. Replaces �S-050 Replaces �S-050
© 2006 Millipore Corporation. All rights reserved.
Specification Sheet NovAseptic
Estapor®White Microspheres
antigen in a latex-enhanced IT
immunoassay.
Figure 5. TEM pictures of Estapor® Carboxylated Microspheres, K1-010 (lot M4263, left) and K1-050 (lot M4266, right).
Small, Medium and Large
PureProteome Nickel Magnetic Beads
2-4 ml samples (cont.)
Amicon Ultra-4 Centrifugal Filter Unit with Ultracel-50 membrane 50 24 UFC8 050 24
Amicon Ultra-4 Centrifugal Filter Unit with Ultracel-100 membrane 100 24 UFC8 100 24
15 ml samples
Fast-Trap Adeno Associated Virus (AAV) Purification and Concentration Kit
g for approxi-3.
mately 5 to 10 minutes. Check concen-
trate volume at 5 minutes. Centrifuge
until 200–250 µL remain.
NOTE: Do not allow the volume to be
reduced below 200 µL, as this may
cause the
Performance of Estapor® Microspheres and Hi-Flow™ Plus Membranes in a Lateral Flow Assay for Human Chorionic Gonadotropin (hCG)
0.190 > 300
K1-030 0.288 0.270–0.330 200–500
R&D Sample 1* 0.418 * *
R&D Sample 2* 0.422 * *
K1-050 0.478 0.450–0.530 1–10
Note: K1-010, K1-030 & K1-050 are available in black, blue
NovAseptic Valve, Valve Body, Take off Valve, CIP/ SIP
Valve is designed for minimum dead
leg, complete drainability and high chemical resistance.
NU#050/5##-211
Catalogue Numbers:
C
E1 E2
A
3
A
2
B
C
D1
Tube1
Tube2 Tube2
F
Microfil User Guide
Strep, 5 g bottle 1/pk MB00 00T TC
Yeast and Mold (m-Green Broth)
2 mL plastic ampoules 50/pk M000 00P 2M
dehydrated, 110 g (1/4 lb.) 1/pk MB00 000 0Y
50 mL vial with septum cap 8/pk M000 050 8M
PF05801
ESGRO-2i MEDIUM
medium supplemented with LIF, pH 7.1-7.3.
2. GSK3β Inhibitor Supplement: (Catalogue No. SF012-050) One (1) 50 μL vial
containing selective GSK3β inhibitor supplement.
3. Mek1/2 Inhibitor Supplement
Viresolve® Barrier Filters
015 = 0.15 m2
050 = 0.5 m2
100 = 1.0 m2
Quantity
1 = 1 per package
(capsules only)
9 = 9 per package
(Micro devices only)
Size
005 =
ESGRO COMPLETE™ SWITCH KIT
supplement (Part No. SF012-050): One (1) 50 µL vial
3. Basal Medium (Part No. SF002-100): One 100 mL bottle
4. Accutase™ Solution (Part No. SF006): One 100 mL bottle
5. Gelatin Solution
ESGRO COMPLETE™ PLUS SERUM-FREE CLONAL GRADE MEDIUM
self-renewal factors, no need to add LIF. pH 7.1-7.3
2. ESGRO Complete Supplement: (Catalogue No. SF012-050) One (1) 50 µL vial
containing selective GSK3β inhibitor supplement.
MEDIA PREPARATION:
Microsphere Coupling - Two-step EDC/Sulfo NHS Covalent Coupling Procedure for Estapor®Carboxyl-modified Dyed Microspheres
Microspheres
Estapor® Carboxyl-Modified Dyed Microspheres
Product Number e.g. K1-020, K1-030, K1-040, K1-050
Buffers
Activation/Coupling Buffer—50 mM MES, pH 6.0
Blocking Buffer—50 mM Tris, pH 8.0, 0.5% (
Chemical Duty Vacuum/Pressure Pump
CFM
0 0 37 1.31 0 0 37 1.31
840 5 25 0.88 0.3 5 32 1.13
670 10 18 0.64 0.7 10 27 0.95
510 15 10 0.35 1.0 15 21 0.74
340 20
Data Sheet - H107
spontaneously beating atrium. 5 The use of cyclodextrins in receptor binding assays is not
recommended.
Mol. Formula: (C6H9O5)7(C3H7O)4.5
Avg. Mol. Wt.: 1396 (anhyd.)
m.p.: >200°C (dec.)
CAS Registry
Estapor®Microspheres for Latex Turbidimetry Immunoassay and Agglutination
23690087
K1 030 0.270 - 0.330 200 - 500 80380014
K1 050 0.450 - 0.530 1 - 10 39424086
K 080 0.750 - 0.890 Plain 23692084
K1 080 0.760 - 0.940 1 - 10 23696087
Product Information Sheet - 11333151001
Product Pack size Cat. No.
BM-Cyclin 37.5 mg
(for 2× 2.5 l
medium)
10 799 050 001
Geneticin (G 418) 1 g
5 g, non-sterile
11 464 981 001
11 464 990 001
Gentamicin (Gentamicin sulfate)
Preparation and Sequencing of Fosmid DNA Templates using the Montage BAC96 Miniprep Kit and Montage SEQ96 & SEQ384 Clean-up Kits and Plates
short-term storage is
required, store the purified samples at –200 C.
V. Typical Data
Representative Electropherogram
5
Results of a ¼ x BDT v3.1
Product Information Sheet - ASP718RO
. 10 814 245 001 1000 units (10 U/�l)
Cat. No. 10 814 253 001 5000 units (10 U/�l)
Cat. No. 11 175 050 001 5000 units , high concentration (40 U/�l)
y Version 19
Content version: February 2016
Store
Viresolve®Barrier Filters
(capsules only)
9 = 9 per package
(PD kit only)
Size
005 = 0.05 m2
015 = 0.15 m2
050 = 0.5 m2
100 = 1.0 m2
PDK = PD kit
Quantitative Lateral Flow Test Using Estapor®Europium Microspheres
COOH
(µeg/g) Cat. No.
F1-Eu- 010 0.130-0.190 >300 80380623
F1-Eu- 030 0.270-0.330 200-500 80380624
F1-Eu- 050 0.450-0.530 1-10 80380625
References:
1. Application Note - Performance of Estapor
Total B-Tubulin Magnetic Bead MAPmates¿
of 5-7 which is approximately 500-800
rpm.
Ensure that the needle probe is clean. This may be achieved by sonication
and/or alcohol flushes.
When reading the assay on Luminex® 200™, adjust
Data Sheet
Fluid for Luminex 100/200™ & FLEXMAP 3D® Systems 20 L SHEATHFLUID
MAGPIX® Calibration Kit 25 uses 40-049
MAGPIX® Performance Verification Kit 25 uses 40-050
Luminex 200™ Calibration Kit (xPONENT
Phospho IκBα (Ser32) Magnetic Bead MAPmate¿
setting of 5-7 which is approximately 500-800 rpm.
Ensure that the needle probe is clean. This may be achieved by sonication and/or
alcohol flushes.
When reading the assay on Luminex® 200™
Total p38α/SAPK2 Magnetic Bead MAPmate¿
setting of 5-7 which is approximately 500-800 rpm.
Ensure that the needle probe is clean. This may be achieved by sonication and/or
alcohol flushes.
When reading the assay on Luminex® 200™, adjust
Total mTOR Magnetic Bead MAPmate¿
and performance
verified with the MAGPIX® Performance Verification Kit (EMD Millipore Catalog # 40-050).
NOTE: These assays cannot be performed on any instruments running Luminex® IS 2.3 or
Luminex
Total GAPDH Magnetic Bead MAPmates¿
setting of 5-7 which is approximately 500-800 rpm.
Ensure that the needle probe is clean. This may be achieved by sonication and/or
alcohol flushes.
When reading the assay on Luminex® 200™
Active Caspase-3 Magnetic Bead MAPmate¿
and performance verified with the MAGPIX®
Performance Verification Kit (EMD Millipore Catalog #40-050).
NOTE: When setting up for a magnetic bead assay using the xPONENT® software,
you must select
Protocol: Total JNK/SAPK1 Magnetic Bead MAPmate¿
setting of 5-7 which is approximately 500-800 rpm.
Ensure that the needle probe is clean. This may be achieved by sonication and/or
alcohol flushes.
When reading the assay on Luminex® 200™, adjust
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