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App Note: LC-MS analysis of plasma samples using Supel™ Swift HLB SPE cartridges
Table 4. Phospholipids monitored
Phospholipid Precursor Product Dwell Time (msec) Collison Energy (V)
1 184 104 65 80
2 496 184 65 80
3 524 184 65 80
4 704 184 65 80
5
App Note: LC-MS analysis of plasma samples using Supel™ Swift HLB SPE cartridges
Table 4. Phospholipids monitored
Phospholipid Precursor Product Dwell Time (msec) Collison Energy (V)
1 184 104 65 80
2 496 184 65 80
3 524 184 65 80
4 704 184 65 80
5
Product Information Sheet - S9420
Welinder, K.J., Eur. J. Biochem., 96, 483 (1979).
3. Methods in Enzymology, 184 (1990). Whole
volume: “Avidin-Biotin Technology.”
4. Dapron, J. and Quinn, T., Sigma production;
published as conference
Product Information Sheet - E5529
such as acetate
buffer (pH 4).
References
1. Chaiet, I., and Wolf, F.J., Arch. Biochem.
Biophys., 106, 1-5 (1964).
2. Bayer, E.A. et al., Methods Enzymol., 184, 80-89
(1990).
3. Bayer
Product Information Sheet - S2415
, Chapters 13-15, pp. 137-162
(1992).
4. Argaraña, C.E. et al., Nucleic Acids Res., 14(4),
1871-1882 (1986).
5. Green, N.M., Methods Enzymol., 184, 51-67
(1990).
6. Green, N.M, Adv. Protein
Product Information Sheet - M0444
Methods Enzymol., 184, 80-89 (1990).
3. Bayer, E.A., and Wilchek, M., Avidin-Biotin
Technology. Methods in Molecular Biology, Vol. 10,
Immunochemical Protocols, p. 137-162 (1992).
4. Green, M.N.
Product Information Sheet - A9754
reaction of acrylamide at alkaline
pH with buffer components and proteins. Anal.
Biochem., 160(1), 184-191 (1987).
3. Angellis, D., et al., Isoelectric focusing of alkaline
phosphatase isoenzymes
Product Information Sheet - G7513
the transition from nitrogen excess growth to
nitrogen starvation. J. Bacteriol., 184(19),
5358-5363 (2002).
4. Honegger, P., et al., Alteration of amino acid
metabolism in neuronal aggregate cultures
Product Information Sheet-10821705001
213 192 184 124 123 104 89 80 64 57 51 21 18 11 8
https://www.sigmaaldrich.com
08
21
.0
36
64
14
70
01
7
3. Results
Product Information Sheet - B6777
3. Horowitz, J.P. et al., J. Med. Chem., 9(3), 447
(1966).
4. Knecht, D.A., and Dimond, R.L., Anal. Biochem.,
136(1), 180-184 (1984).
5. Leary, J.J., et al., Proc. Natl. Acad. Sci. USA,
80
Data Sheet - A8602
Chem.
251, 7423-7430 (1976).
2. Wiest, S.A., et al., Jrnl. Cardio. Pharmacol., 17,
177-184 (1991).
LRP/JK 11/2003
Sigma brand products are sold through Sigma-Aldrich, Inc.
Sigma-
Natrix® CH Micro Chromatography Membrane Devce
122
159
43
0
50
100
150
200
250
300
350
400
450
500
550
Feed M-1 M-2 Natrix® CH Micro
H
C
P
(
p
p
m
)
471
176 184
61
0
50
100
150
200
250
300
350
400
450
500
550
Product Information Sheet - G3126
coli undergoes
the transition from nitrogen excess growth to
nitrogen starvation. J. Bacteriol., 184(19),
5358-5363 (2002).
6. Honegger, P., et al., Alteration of amino acid
metabolism in neuronal
Data Sheet - E0153
Product Description
Recombinant Mouse Ephrin-A2/Fc Chimera consists of
amino acid residues 1-184 of the extracellular domain
of mouse Ephrin-A21 fused by means of a polypeptide
linker to the Fc
Data Sheet - SAB4200696
Chem., 289, 2620–31
(2014).
7. Alilain WJ., et al., Nature., 475, 196-200 (2011).
8. Henrotin Y., et al., Maturitas., 78, 184-87 (2014).
ExtrAvidin is a registered trademark of Sigma-Aldrich Co
Data Sheet - MSST0043
internal standard for bioanalysis of TIMP1 in mass
spectrometry. SILuProt TIMP1 is a protein of
184 amino acids, with a calculated molecular mass of
20.7 kDa.
TIMP-1 is glycoprotein that is overexpressed
Product Information Sheet - N2765
Diagnosis and Pathogenesis of Immunobullous
Disorders". King’s College London, Ph.D.
dissertation, p. 184 (2015).
9. Sordé, Laetitia, "Anti-inflammatory effects of
intravenous immunoglobulin (IVIg): what
HybridSPE-Precipitation Technology
recovery
Clenbuterol
Phospholipids
Max 775 CPS
Max 327 CPS
Min
1 2 3 4 50
C
P
S
300
200
100
HybridSPE-PPT Plate and Vacuum Manifold
Description Qty.
Data Sheet - T1830 - Lot SLBB2534
factors and
Tie receptor tyrosine kinases in renal vascular
development. Pediatr. Nephrol., 16, 177-184
(2001).
2. Peters, K.G. et al., Functional significance of Tie2
signaling in the adult vasculature
Data Sheet - T1830 - Lot 109K0797
factors and
Tie receptor tyrosine kinases in renal vascular
development. Pediatr. Nephrol., 16, 177-184
(2001).
2. Peters, K.G. et al., Functional significance of Tie2
signaling in the adult vasculature
Acrylamido Buffers
- 1011 200 375 13 147 105 127 15 1546
3-11 1492 - 64 46 147 31
Product Information Sheet - MAK453
Table 1.
Table 1.
Preparation of Working Reagent
Reagent
Working
Reagent
Assay Buffer 184 L
Glutathione Reagent 1 L
CDNB 1 L
2. Add 180 L of Working
MOUSE ANTI-LAMININ-5 ALEXA FLUOR® 488 CONJUGATED
cells and its association with stromal fibrosis in lung adenocarcinomas. Jpn. J. Cancer
Res. 92(2): 184-192.
Seftor, RE et al (2001). Cooperative Interations of Laminin 5 gamma2 chain, Matrix
metalloproteinase
Data Sheet - F5055 - Lot 061M0759
Number 061M0759:
>90% (densitometry)
Figure 2.
Specific Activity of Lot Number 061M0759:
184 nmole/min/mg
Procedure
Preparation Instructions
Kinase Assay Buffer – 25 mM MOPS, pH 7.2, 12.5
Data Sheet - P9857
Anti-Potassium Channel Kv3.2 is developed in rabbit
using a synthetic peptide corresponding to amino acids
184-204 of rat potassium channel Kv3.2 (with additional
C-terminal cysteine) as immunogen.1,2 The antibody
Product Information Sheet - A6362
Olson, M.O. et al., Nature, 228, 438-442 (1970).
4. Brayer, G.D. et al., J. Mol. Biol., 131(4), 743-775
(1979).
5. Fujinaga, M. et al., J. Mol. Biol., 184(3), 479-502
(1985).
6. Bauer, C.A. et al
Product Information Sheet -RAB0166
Curr. Opin. Cell Biol., 11, 184-189 (1999).
2. Zwick, E. et al., Trends Pharmacol. Sci., 20, 408-412 (1999).
3. Cooper, J.A., and Howell, B., Cell, 73, 1051-1054 (1993).
4. Riedemann, J. et al., Biochem
Product Information Sheet - RAB0155
).
2. Hackel, P.O. et al., Curr. Opin. Cell Biol., 11, 184–
189 (1999).
3. Levkowitz, G. et al., Mol. Cell, 4, 1029–1040
(1999).
4. Zwick , E. et al., Trends Pharmacol. Sci., 20
Product Information Sheet-S6940
Rights Reserved.
S6940pis Rev 04/24
4
References
1. Wilchek, M., and Bayer, E.A., Avidin-Biotin
Technology. Meth. Enzymol., 184 (1990).
Dosing considerations and impacts on the clarification of mammalian cell culture feed streams
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