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Data Sheet - A0310
.
2. Constantinople CM., et al., J Comp Neurol., 516,
291-311 (2009).
3. Berbari NF., et al., Proc Natl Acad Sci U S A.,
105, 4242-6 (2008).
4. Omelchenko N. and Sesack SR., J Comp Neurol.
Data Sheet - S9072
expression extending from AEG
syndrome to absence of ocular involvement. Am. J.
Med. Genet. 143A: 289-291 (2007).
BKR,PHC 04/08-1
Sigma brand products are sold through Sigma-Aldrich, Inc.
Sigma-Aldrich
Anti-TBX3, clone 8H3 - Data Sheet
S A. 103(45):16788-16793
Govoni KE (2006) Am J Physiol Endocrinol Metab. 291(1):E128-E136.
Vance KW (2005) Cancer Res. 65(6):2260-2268.
Important Note: During shipment, small volumes of
RABBIT ANTI-ANION EXCHANGER 1 (AE1)
(1989) J Biol Chem. 264:8185; Kopito RR et al (1985) Nature 316:234;
Raida M et al (1989) BBA 980:291; Lux SE et al (1989) PNAS 86:9089; Tanner MJ et al
(1988) Biochem. J. 256:703.
Important Note
Data Sheet - T8665
and Chudzik-Kozłowska, J., Acta
Alimentaria, 43(2), 193-291 (2014).
5. Cole, L.A. et al., Clin. Chem., 45(12), 2109-2119
(1999).
6. Figini, M., and Canevari, S., “Isolation of Human
Monoclonal
MOUSE ANTI-A2B5
.K. et al. (1988) J. Clin. Invest. 71:9.
11. Bottenstein, J.E. et al. (1988) J. Neurosci. Res. 20:291.
12. Suzumura, A. and Slerberg, D.H. (1989) Brain Res. 480:51.
13. Dubois-Dalcq, M. et al. (1990)
Data Sheet - S2438
times, 300 µl per well, using
PBS with 0.05% TWEEN 20.
6. Prepare a working solution of the streptavidin-HRP
polymer conjugate by diluting 1:200 in PBS with
0.05% TWEEN 20. To assess background from
non-specific
Data Sheet - T5525
14618
(2010).
4. Szymkiewicz, A., and Chudzik-Kozłowska, J., Acta
Alimentaria, 43(2), 193-291 (2014).
5. Rosenqvist, E. et al., “Determination of Antibody
Responses to Meningococcal Antigens
MultiScreen Filter Plates with Ultracel®-10 Membrane
125
90 ABS 108 84 72 65 65 75 92 105
BSA 271 268 268 274 269 269 267 265
Buffer 291 295 292 293 291 293 291 287
Cyto c 269 266 268 266 265 265 264 266
FBS 154
N21 MEDIUM SUPPLEMENT (50X)
Component Amount
DMEM/F12, with L-glutamine (Millipore Cat. No. DF-041-B) 291 mL
N-Base medium (Millipore Cat. No. N014-B) 291 mL
N21 medium supplement (Millipore Cat. No. SCM081) 10 mL
Neuro2 medium
Data Sheet - P9542
alpha and epsilon pseudosubstrate peptides
References
1. Hug, H., and Sarre, T.F., Biochem. J., 291, 329-343
(1993).
2. Nishizuka, Y., Science, 233, 305-312 (1986).
3. Jaken, S., Current Opinion
Data Sheet - P8538
Sarre, T.F., Biochem. J., 291, 329-343
(1993).
4. Kazanietz, M.G., et al., Mol. Pharmacol., 44, 298-
307 (1993).
5. Newton, A.C., J. Biol. Chem., 270, 28495-28498
(1995).
6. Epand, R.M., Anal. Biochem
Data Sheet - P6662
nm on a multiwell plate reader.
6. If you cannot read the plate immediately, the
reaction may be stopped by the addition of 50 µL
of 3 M HCl or 3 M H2SO4 per 200 µL of reaction
Data Sheet - P6787
nm on a multiwell plate reader.
6. If you cannot read the plate immediately, the
reaction may be stopped by the addition of 50 µL
of 3 M HCl or 3 M H2SO4 per 200 µL of reaction
Corning Synthemax-R Surface for Research
3336
Tel: (+86) 21 6141 5566
Fax: (+86) 21 6141 5567
Czech Republic
Tel: (+420) 246 003 200
Fax: (+420) 246 003 291
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Tel: (+45) 43 56 59 00
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Finland
Tel: (+358) 9 350 9250
Fax
Product Information sheet - T8665
, and Chudzik-Kozłowska, J., Acta
Alimentaria, 43(2), 193-291 (2014).
5. Cole, L.A. et al., Clin. Chem., 45(12), 2109-2119
(1999).
6. Figini, M., and Canevari, S., Methods Mol. Biol.,
178, 207
Data Sheet - SRP5035
mice. Neuron, 38, 291-303
(2003).
PRECISIO is a registered trademark of Sigma-Aldrich
Co. LLC.
TD,MAM 10/11-1
2011 Sigma-Aldrich Co. LLC. All rights reserved. SIGMA-ALDRICH is a trademark of Sigma-Aldrich
Data Sheet - P8806
nm on a multiwell plate reader.
6. If you cannot read the plate immediately, the
reaction may be stopped by the addition of 50 µL
of 3 M HCl or 3 M H2SO4 per 200 µL of reaction
Parteck® LM Excipient System Reduce complexity in your formulation and optimize results
95% Parteck
® LM High + 5% Propranolol HCl 500 mg; Ø 11mm, flat & facetted 133 1.74 0.22 259 - 291 254 0.58
Sildenafil 50 mg
40% Parteck® LM High + 17.78% Sildenafil-citrate +
36.22 MCC, Type 102
Data Sheet - P4664
nm on a multiwell plate reader.
6. If you cannot read the plate immediately, the
reaction may be stopped by the addition of 50 µL
of 3 M HCl or 3 M H2SO4 per 200 µL of reaction
Data Sheet - P8787
nm on a multiwell plate reader.
6. If you cannot read the plate immediately, the
reaction may be stopped by the addition of 50 µL
of 3 M HCl or 3 M H2SO4 per 200 µL of reaction
Data Sheet - P3804
nm on a multiwell plate reader.
6. If you cannot read the plate immediately, the
reaction may be stopped by the addition of 50 µL
of 3 M HCl or 3 M H2SO4 per 200 µL of reaction
Data Sheet - P1063
nm on a multiwell plate reader.
6. If you cannot read the plate immediately, the
reaction may be stopped by the addition of 50 µL
of 3 M HCl or 3 M H2SO4 per 200 µL of reaction
Data Sheet - SRP5316
apoptosis and NF-kappa-B nuclear
translocation and localizes to mitochondria. FEBS
Lett., 473, 285-291 (2000).
2. Oberst, A. et al., Catalytic activity of the caspase-8-
FLIP(L) complex inhibits RIPK3
Product Information Sheet - A7480
twice
with PBS with a centrifugation after each wash.
6. Incubate the cells overnight at 2–8 °C in the
250 µM FLAG Phosphine Solution.
(200 µL/1.5 × 106 cells).
Notes:
• For labeling cytosolic proteins
XP725 Antibody Number-Lot Number 071M4826
260 281 282 283 284
3.6 213 214 215 216 237 238 239 240 261 262 263 264 285 286 287 288
4.1 289 290 291 292 313 314 315 316 337 338 339 340 361 362 363 364
4.2 293 294 295
Product Information Sheet - A7605
twice
with PBS with a centrifugation after each wash.
6. Incubate the cells overnight at 2–8 °C in the
250 µM FLAG Phosphine Solution.
(200 µL/1.5 × 106 cells).
Notes:
• For labeling cytosolic proteins
Product Information Sheet - A7355
twice
with PBS with a centrifugation after each wash.
6. Incubate the cells overnight at 2–8 °C in the
250 µM FLAG Phosphine Solution.
(200 µL/1.5 × 106 cells).
Notes:
• For labeling cytosolic proteins
Data Sheet - P6912
nm on a multiwell plate reader.
6. If you cannot read the plate immediately, the
reaction may be stopped by the addition of 50 µL
of 3 M HCl or 3 M H2SO4 per 200 µL of reaction
Product Information Sheet - GPHOS1
twice
with PBS, with a centrifugation after each wash.
6. Incubate the cells overnight at 2–8 °C in the
250 µM FLAG-Phosphine Solution.
(200 µL/1.5 × 106 cells).
Lysis of Cells with RIPA Buffer
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