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2nd Generation Material - 1st Class PerformanceVol. 2: Comparison of Quality and Safety Parameters, Cytotoxicity and Leaching Levels of epT.I.P.S.® BioBased and epT.I.P.S.® Standard
tips
APPLICATION NOTE I No. 477 I Page 3
The following pipette tips were evaluated in all
three assays:
> epT.I.P.S.® BioBased, Biopur®, Reloads, 2-200 µL
(order no. 0030 075
Cathepsin B Detection Kit
Bank U., et al. 2000. Adv. Exp. Med. Biol. 477, 349.
Buhling F., et al. 2000. Adv. Exp. Med. Biol. 477, 241.
Gerber A., et al. 2000. Adv. Exp. Med. Biol. 477, 287.
Kas J. et al. 2000.
RABBIT ANTI-CALCIUM CHANNEL BETA 3 AFFINITY PURIFIED POLYCLONAL ANTIBODY
purified peptide corresponding to amino acid residues 463-477 of rabbit Caβ3
(Accession number P54286).
APPLICATIONS: Western blot: 1:200-1:300 using ECL on rat brain membranes.
Dilutions should
RABBIT ANTI-CALCIUM CHANNEL BETA 3 AFFINITY PURIFIED POLYCLONAL ANTIBODY
purified peptide corresponding to amino acid residues 463-477 of rabbit Caβ3
(Accession number P54286).
APPLICATIONS: Western blot: 1:200-1:300 using ECL on rat brain membranes.
Dilutions should
Human IgG Fc Control Protein, recombinant protein
Ther. 2008 Mar;7(3):538-47.
2. J Immunol. 2008 Feb 15;180(4):2099-106.
3. J Biol Chem. 2007 Nov 30;282(48):34748-57. Epub 2007 Oct 2.
4. AIDS Res Hum Retroviruses. 2006 Jun;22(6):477-90.
5. J Neurosci
Product Information Sheet - N4160
µg of protein, to the Control 2
tube and the Sample tubes.
7. Add sufficient assay buffer to each tube to bring
the final volume to 200 µl.
8. Mix each tube for 2 hours at 4 °C in a
Product Information Sheet - CS0370
Adv. Exp. Med. Biol., 477,
241-254 (2000).
6. Turk B, Turk D, Turk V. Lysosomal cysteine
proteases : more than scavengers. Biochim.
Biophys. Acta., 1477, 98-111 (2000).
7. Gerber, A. et al.
Data Sheet - A1477
94,
10868-10872 (1997).
17. MacDonald, N.J., et al., Biochem. Biophys. Res.
Commun., 264, 469-477 (1999).
18. Marta, C. et al., J. Mol. Biol., 318, 1009-1017
(2002).
Sold under license from
Anti-Calcium Channel (b3 Subunit) (Cacnb3l) antibody produced in rabbit (C1978)
Channel (β3 Subunit) is developed in
rabbit using a synthetic peptide corresponding to amino
acids 463-477 of the β3 subunit of rat brain voltage-
gated calcium channel (VGCC, Caβ3) (with additional
N-terminal
MOUSE ANTI-LAMININ-5 ALEXA FLUOR® 488 CONJUGATED
50(suppl2): 7-14.
Important Note: During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For
products with volumes of 200 μL or less
Data Sheet - S7944
).
3. Krishtal, O., The ASICs: Signaling molecules?
Modulators?, Trends in Neurosciences, 26, 477-
483 (2003).
4. Deval, E., et al., ASIC2b-dependent Regulation of
ASIC3, an Essential Acid-sensing
Data Sheet - S5070
).
3. Krishtal, O., The ASICs: Signaling molecules?
Modulators?, Trends in Neurosciences, 26, 477-
483 (2003).
4. Deval, E., et al., ASIC2b-dependent regulation of
ASIC3, an essential acid-sensing
Data Sheet - D0942
, 1587,
164-173 (2002).
6. Michnick, S.W., et al., Curr. Opin. Struct. Biol., 11,
472-477 (2001).
7. Pelletier, J.N., et al., Nat. Biotechnol., 17, 639-640
(1999).
8. Mossner, E., et al., J.
Product Information Sheet - D1067
1587,
164-173 (2002).
6. Michnick, S.W., et. al., Curr. Opin. Struct. Biol., 11,
472-477 (2001).
7. Pelletier, J. N., et. al., Nat. Biotechnol., 17, 639-640
(1999).
8. Mossner, E., et. al
Detergent Optimization for Total Protein Extraction from Chinese Hamster Ovary (CHO) Cells
strips IPG strips
2% C7BzO 317 477
1% ASB-14/2% C7BzO 270 404
1% ASB-14/1% C7BzO 287 397
2% ASB-14/1% C7BzO 242 372
4% CHAPS 274 397
Discussion
Challenges for proteomic sample preparation optimization
Multiplex immunoassay detection of autoimmune disease autoantibodies in serum and plasma
6.06 -
5 373 - 9.78 -
6 477 - 7.83 -
7 15889 + 409.25 +
8 476 - 13.79 -
MILLIPLEX® MAP Competitor ELISA
SLE patient
y = 0.0253x - 10.452
R = 0.985
0
50
100
150
200
250
300
350
400
450
0
XP725 Antibody Number-Lot Number 071M4826
425 426 427 428 449 450 451 452 473 474 475 476
5.6 405 406 407 408 429 430 431 432 453 454 455 456 477 478 479 480
6.1 481 482 483 484 505 506 507 508 529 530 531 532
Product Information Sheet - S0501
Research, Eds. Dawson, R.M.C. et al., Third Edition, Clarendon Press, Oxford,
1987, pp. 457, 467, 477, 490, 491, 492, 500.
36. Marks, G.S. et al., Can. J. Physiol. Pharmacol. 69, 889, 1991.
37. Rao, D.
Data Sheet - MWSDS17S
.
6. Ammonium Persulfate Solution: Dissolve
approx. 200 mg ammonium persulfate, Product
No. A3678, in 10 ml water. Prepare fresh daily.
7. Fixative Solution: Prepare by combining:
5-Sulfosalicylic
Product Information Sheet - CS0360
Adv. Exp. Med. Biol., 477,
241-254 (2000).
6. Turk B, Turk D, Turk V. Lysosomal cysteine
proteases : more than scavengers. Biochem.
Biophys. Acta., 1477, 98-111 (2000).
7. Inaoka, T., et al.
Bulletin - LUM1
enzymatic
dephosphorylation, CSPD decomposes, releasing a
constant emission of light with a maximum at 477 nm.
The results can be conveniently imaged on x-ray film
with exposure times of 5-120 minutes. The
Product Information Sheet - CS0350
Adv. Exp. Med. Biol., 477,
241-254 (2000).
6. Turk B, Turk D, Turk V. Lysosomal cysteine
proteases : more than scavengers. Biochim.
Biophys. Acta., 1477, 98-111 (2000).
7. Johnson, D. A., et
Product Information Sheet-11655884001
leads to the metastable phenolate anion which
decomposes and emits light at a maximum wavelength of 477 nm. The luminescent light emission is recorded on
X-ray film or Lumi-Film*, or by suitable cameras
Product Information Sheet-11755633001
leads to the metastable phenolate anion which
decomposes and emits light at a maximum wavelength of 477 nm. The luminescent light emission is recorded on
X-ray film or Lumi-Film, or by suitable cameras
Product Information Sheet - B6166
Jagow, G., Anal. Biochem. 166: 368-379 (1987)
Swank, R.T., & Munkres, K.D., Anal. Biochem. 39: 462-477 (1971)
Kratzin, H.D., Wiltfang, J., Karas, M., Neuhoff, V. and Hilschmann, N.,
Anal. Biochem., 183
Product Information Sheet - 11759779001
AP). The
resulting instable dioxetane anion decomposes and
emits light at a maximum wavelength of 477 nm. Chemi-
luminescence-enhancing reagents improve the quantum
yield of the excited state by more
Mobius®SensorReady Technology: A Flexible Approach for Monitoring Mobius®Single-use Bioreactors
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Product Information Sheet - PKH26PCL
et al., Wound Repair Regen, 18,
245-253 (2010).
27. Belyaev, N.N. et al., Nature immunol., 11, 477-
485 (2010).
28. Jambou, R. et al., PLoS Pathogens, 6,
e1001021 (2010).
29. Ely P, et al., Blood
Product Information Sheet-11363514910
dephosphorylation of CSPD by alkaline phosphatase leads to a light emission at a maximum
wavelength of 477 nm which is recorded on X-ray film or Lumi-Film*.
– The chemiluminescent signal from CSPD persist
FlowCellect™ Cell Cycle Checkpoint ATM DNA Damage Kit
. Nat Protoc.; 3(7):1187-93.
6. Wu, X., et al. (2000). ATM phosphorylation of Nijmegen breakage syndrome protein is required
in a DNA damage response. Nature; 405:477-482.
7. Zhao, H., et al.
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