200-540-9 | Sigma-Aldrich

Data Sheet-SCT010

LuminiCell Tracker™ 540 200 nM in 1 X PBS, pH 7.4 2-8 °C When store as instructed, stable for at least 6 months 423 nm 540 nm LuminiCell Tracker™

Cathepsin B Detection Kit

. 15. If cells are to be stained with AO (Section 9) instead of Cathepsin B fluorogenic substrate, pipette the AO reagent stock 1:2000 to 1:200 (0.05 – 0.5% v/v) into the final cell suspension. (Because

Product Information Sheet - CS0360

480 nm excitation/540 nm emission lengths requires a wash step with PBS to remove any excess AO to reduce excessive brightness. 9. Wash procedure: • Gently pellet cells at 200 X g for 3-8 minutes

Product Information Sheet - CS0370

480 nm excitation/540 nm emission lengths requires a wash step with PBS to remove any excess AO to reduce excessive brightness. 9. Wash procedure: • Gently pellet cells at 200 X g for 3-8 minutes

Product Information Sheet - CS0350

480 nm excitation/540 nm emission lengths requires a wash step with PBS to remove any excess AO to reduce excessive brightness. 9. Wash procedure: • Gently pellet cells at 200 X g for 3-8 minutes

Purification of Process Solutions with Millistak+ A Series Filters -- A simple and efficient alternative to powdered carbon

1.5 0 200 400 600 800 Volume, ml R ed uc tio n in A bs or ba nc e 2430 LMH 540 LMH �� !�� $�� 9�� "� 9��

Poster: Montage PCRµ96 and SEQ96: A Total Solution for Sequencing PCR-derived Templates

Displaying Size Phred-30 (Phred 20 > 300) (Distance from Primer) Primer Plus 1 1/4 540 + 33 100% 2.1 75% 1/8 540 + 31 100% 1.2 96% 1/16 555 + 4 100% 1.0 100% 1/32 552 + 15 100% 1.8 79%

Product Information Sheet - A3759

para- aminobenzoic acid (PABA): a quantitative test of liver function. Clin. Biochem., 28(5), 531-540 (1995). 3. Chan, K., et al., Direct and simultaneous high- performance liquid chromatographic

Product Information Sheet - CKH200A

times ê Add 200 µl Substrate Solution to each well. Incubate 20 min. RT ê Add 50 µl Stop Solution to each well. Read at 450 nm within 30 min.

BrdU Cell Proliferation Assay 200 Tests

-well microplate reader with 450-540 nm or 450-595 nm dual wavelength or 450 nm single wavelength filter. 8. Tissue culture microplate (96 well culture dish) 9. Sterile reagent troughs 10.

MOUSE ANTI-HUMAN MEROSIN (M-CHAIN)

Disord. 7: 211-216. 7. Noam, I. et al. (1997) Hum Genet. 99: 535-540. 8. Noam, I. et al. (1997) Neuromusc. Disord. 7: 176-179. 9. Tan, E. et al. (1997)

User Guide-RAB1049

serves as the zero standard (0 ng/mL). 200 µL 200 µL 200 µL 200 µL 200 µL 200 µL Std 1 Std 2 Std 3 Std 4 Std 5 Std 6 Std 7 0 Std Diluent volume

MOUSE ANTI-HUMAN LAMININ (α5-CHAIN)

(1995) Neuromusc. Disord. 5(4): 307-316. 9. Taylor, J. et al. ( 1997) Neuromusc. Disord. 7: 211-216. 10. Noam, I. et al. (1997) Hum Genet. 99: 535-540. 11. Noam, I. et al. (1997) Neuromusc. Disord

Product Information Sheet-RAB0245

gentle mix. Add 60 µL IL-10 standard (50 ng/mL) from the vial of Standard Protein, into a tube with 540 µL 1X Assay Diluent D or 1X Assay Diluent B to prepare a 5,000 pg/mL standard solution. Pipette 300

User Guide-RAB0245

gentle mix. Add 60 µL IL-10 standard (50 ng/mL) from the vial of Standard Protein, into a tube with 540 µL 1X Assay Diluent D or 1X Assay Diluent B to prepare a 5,000 pg/mL standard solution. Pipette 300

Data Sheet-ecm531

Materials Required (Not supplied) • Multi-channel or repeating pipettes • Microplate reader (540-570 nm detection) or spectrophotometer • CO2 incubator appropriate for subject cells • Hemacytometer

Kits and Reagents for Metabolic and Dietary Research

m-anisidine (ESPA) to produce a quinoneimine dye that shows an absorbance maximum at 540 nm. The increase in absorbance at 540 nm is directly proportional to triglyceride concentration of the sample. Many

Data Sheet-ecm540

microscope for counting cells. • Trypan Blue or equivalent viability stain. • Microplate reader (540-570 nm detection) or spectrophotometer.

QCM™ Endothelial Cell Invasion Assay (24 well, colorimetric)

room temperature on an orbital shaker. 9. Transfer 100 µL of the solution to the included 96-well Stain Quantitation Plate and read absorbance at 540- 570 nm. 5

BRAND® Insert 2in1 supports the cultivation of RhE used for skin corrosion tests

Measurements were performed in transparent flat bottom microplates using a microplate spectrophotometer at 540 -570 nm To test whether the RhE models cultivated in BRAND insert 2in1 can also be used to distinguish

COX-2 ELISA Kit Flyer

with reference wavelength set at 540-600 nm) and comparing those values with the standard curve. The COX-2 ELISA Kit is: • Highly sensitive: detects COX-2 at 2.5–200 ng/ml • Quantitative: includes

Product Information Sheet - MAK320

reduction of resazurin to the fluorescent dye resorufin (ex = 540 nm/em = 590 nm). Components The kit is sufficient for 200 fluorometric reactions D2HG Standard 100 l 10 mM 2-Hydroxyglutarate

Data Sheet - E8777

Hek3 Product Description Recombinant Mouse EphA8/Fc Chimera consists of amino acid residues 1-540 of the extracellular domain of mouse EphA81 fused by means of a polypeptide linker to the Fc portion

Product Information Sheet - N9389

Microbiology (Washington, D.C.), pp. 99-109 (1986). 4. Wei, J.S. et al., Clin. Chem., 39(3), 540-543 (1993). 5. Gioria, R. et al., Plant Pathology, 51, 127-133 (2002). 6. Owen, M.A.G. et

MAK407 - Technical Bulletin

and reacts with Griess Reagent 1 and 2 to generate a colored product with a strong absorbance at 540 nm. The assay is simple, sensitive and high-throughput adaptable and can detect as low as 5 U of

The Active Siteﾙ Volume 4, Number 1

Red-IETD-FMK) Fluorescent marker: Red-IETD-FMK (Ex. max.: ~540 nm; Em. max.: ~570 nm) Inhibitor included: Z-VAD-FMK Cat. No. QIA114 1 Kit Caspase-9 Detection Kit (FITC-LEHD-FMK) Fluorescent marker:

QCM™ 3 mm Endothelial Cell Migration Assay – Fibronectin, Colorimetric

the included 96-well microtiter plate Stain Quantification plate and read absorbance 540 – 570 nm. 9 Note: After extraction of the stain, if results suggest that individual cell counting

Adipolysis Assay Kit

plate. 17. Add 200 µL Free Glycerol Assay Reagent per well. 18. Incubate for 15 minutes at room temperature. 19. Read absorbance in spectrophotometric plate reader at 540 nm. A wavelength of

Data Sheet - 17951

concentration)

Data Sheet - 89952

concentration)