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Determination of 7 Nitrosamines in Drinking Water

Nitrosamines analysis by solid phase extraction (SPE) and subsequent gas chromatography-tandem mass spectrometry (GC-MS/MS) according to GB 5750.10-2023.

Protein Conformation Array Multiplexing

Monoclonal antibodies (mAbs) lead biologics growth, predicted $125 billion worldwide sales by 2020, emphasizing their therapeutic significance.

Estimation of Proline in Fruit and Vegetable Juices

Spectrophotometric determination of proline by the Ninhydrin method, per EN 1141 and German Food and Feed Code §64 LFBG 31.00-7.

RNA Therapeutics & Delivery USA

Visit us at booth #7 to learn about our solutions to help you overcome your mRNA manufacturing and LNP formulation challenges.

cOmplete™, Mini, EDTA-free Protocol

Dissolve one tablet in 10 ml aqueous buffer or water. If high proteolytic activity is present, dissolve one tablet in 7 ml buffer.

Scepter™ 3.0 Cell Counter – How it Works

Scepter™ counting is 7 to 10 times faster than hemocytometry-and also faster than other automated counters. Learn more about how this amazing technology works

Enzymatic Assay of Cathepsin B

Cathepsin B is a lysosomal cysteine proteinase with broad specificity. This protocol uses Nα–CBZ–Arg–Arg–7–amido–4–methylcoumarin as the substrate for fluorometric detection of Cathepsin B activity.

Assay of Betamethasone Sodium Phosphate by HPTLC

Development of an HPTLC based method, using Silica Gel 60 F254 plate, to determine Betamethasone Sodium Phosphate over a concentration range of 1 to 7 µg/spot.

Ultrapure Water for Sensitive LC-MS Analysis of Pesticides

Pesticides analysis by LC-MS needs pesticide-free reagent water, which can be reliably delivered by Milli-Q® IQ 7 ultrapure water systems. See data.

Obtaining Ultrapure Water for Sensitive PFAS Analysis by LC-MS

Sensitive PFAS testing can be performed with ultrapure water from a Milli-Q® IQ 7 system, which eliminates detectable PFAS for reliable LC-MS/MS results. See the science, see the proof.

In Vitro Differentiation of Human PBMC and CD34+ Derived Monocytes into Mature CD83+/CD14- Dendritic Cells

Generate mature human dendritic immune cells from PBMCs or CD34+ stem cell derived monocytes in 7 days in defined and serum-free dendritic cell culture media. Explore over 350 PromoCell products.

3D Mammosphere Culture Media

3D mammosphere culture protocol used to expand human MCF-7 and MDA-MB-231 breast cancer cells in serum-free/xeno-free stem cell culture media. Explore over 350 PromoCell products on Sigma.com.

Cleaning Up Samples using 2-D Clean-Up Kit

2-D Clean-Up Kit from Cytiva is designed to prepare samples that would otherwise produce poor 2-D results.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

Functional Biomaterials Synthesized by Double-Head Polymerization Agents

Over the past two decades, the rapid advance of controlled living polymerization (CLP) techniques.

Sample Preparation for Chromatographic Purification

This page discusses various aspects of sample preparation for chromatographic purification.

Isotopic Labeling for NMR Spectroscopy of Biological Solids

Isotopic Labeling for NMR Spectroscopy of Biological Solids;

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

Intermetallic Hydrides with High Dissociation Pressure

The rates of reaction for intermetallic compounds differ significantly from those for individual metals, and therefore, intermetallic hydrides are prospective materials for hydrogen storage and distribution.

Thermal Transitions of Homopolymers: Glass Transition & Melting Point

Literature values for the glass transition temperature, (Tg), and melting temperature, (Tm), are given for the more common homopolymers.

Chemical Synthesis of Monodisperse Magnetic Nanoparticles

Magnetic nanoparticles have attracted tremendous attention due to their novel properties and their potential applications in magnetic recording, magnetic energy storage and biomedicine.

Peptide Mapping LC-MS/MS Analysis Workflow for Adalimumab

An optimized LC-MS/MS based workflow for low artifact tryptic digestion and peptide mapping of monoclonal antibody, adalimumab (Humira) using filter assisted sample preparation (FASP).

Self-Propagating Reactions Induced by Mechanical Alloying

An article concerning self-propagating reactions induced by mechanical alloying, presented by Sigma-Aldrich.com.

Colby Group – Professor Product Portal

Prof. David Colby's laboratory is developing synthetic methods to enable the efficient modification of complex molecules, such as natural products, as well as the production of fluorinated compounds. They have focused on understanding how to cleave bonds in order to

Mesoporous Silica Applications

Mesoporous silica used in wastewater remediation, air cleaning, catalysis, bio-catalysis, and drug delivery.

Rapid Determination of Protein Binding Affinity Using Solid Phase Microextraction

Accurate protein binding affinities were measured using bioSPME more rapidly than membrane dialysis (RED) techniques. Binding affinities correlated to the reference values.

Rehydration Solutions

This page describes typical rehydration solutions for use in gel electrophoresis with Cytiva products.

Imprint® Chromatin Immunoprecipitation Kit (CHP1) Protocol

The Sigma Imprint Chromatin Immunoprecipitation Kit uses a plate based system to allow rapid ChIP assays in a high throughput format

Sorting Carbon Nanotubes and Their Biological Applications

Carbon nanotubes (CNTs) have received much attention since their discovery in 1991 by Sumio lijima1 due to their excellent mechanical, electrical, and optical properties.

Buchwald Phosphine Ligands

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