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Chrombook 2015 - The world of chromatography in your hands
2
1
2
1
2 1.
2.
3.
Intensity [x=107]
0
0
0 100 200 300 400 500 600
[m/z]
Ketoprofen
M=254
-MS, 6.4-7.2 min, background subtracted
-MS, 7.5-8.2
Biologics 30.1
Anti-IKKε/IKK-i (701-716), 400004 Immunogen used was a synthetic peptide corresponding to 100 µg 219
Human (Rabbit) amino acid residues 701 - 716 of human IKKε/IKK-i.
Recognizes an ~80 kDa band in
Analytical Procedures and Annexes - 1.73028 - EN
250 m-1 254 nm 10-mm cell Method No. 300
range: 0.3 – 125.0 m-1 254 nm 20-mm cell Method No. 300
0.1 – 50.0 m-1 254 nm 50-mm cell Method No. 300
Spectral Absorption Coefficient
α(254)
Complete Monograph Methods - Solutions for regulated pharmaceutical instrumental analysis: Methods with HPLC, FTIR, KF, AAS and ICP 2015-1
62932 219
SLOPE 16616 2944
LOD 12.5 0.25
LOQ 38 0.75
Raloxifene RS CRaloxifene
y = 16616x + 62932
R² = 0.9999
0
40000000
80000000
120000000
160000000
200000000
Analytical Procedures and Annexes - 1.73027 - EN
250 m-1 254 nm 10-mm cell Method No. 300
range: 0.3 – 125.0 m-1 254 nm 20-mm cell Method No. 300
0.1 – 50.0 m-1 254 nm 50-mm cell Method No. 300
Spectral Absorption Coefficient
α(254)
User Guide Equinox SOP Software 2.1 for Windows XP
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User Guide - 1.73018 - Analytical Procedures and Annexes
250 m-1 254 nm 10-mm cell Method No. 300
range: 0.3 – 125.0 m-1 254 nm 20-mm cell Method No. 300
0.1 – 50.0 m-1 254 nm 50-mm cell Method No. 300
Spectral Absorption Coefficient
α(254)
User Guide - 1.73017 - Analytical Procedures and Annexes
250 m-1 254 nm 10-mm cell Method No. 300
range: 0.3 – 125.0 m-1 254 nm 20-mm cell Method No. 300
0.1 – 50.0 m-1 254 nm 50-mm cell Method No. 300
Spectral Absorption Coefficient
α(254)
Brochure: The Supelco® HPLC and UHPLC Column Selection Guide
2
1
2
1
2 1.
2.
3.
Intensity [x=107]
0
0
0 100 200 300 400 500 600
[m/z]
Ketoprofen
M=254
-MS, 6.4-7.2 min, background subtracted
-MS, 7.5-8.2
Perfection in every dimension 2014. Inorganic reagents Catalogue
Eur 104928 75
Potassium carbonate-1.5-hydrate 99.995 Suprapur® 104926 219
Potassium chlorate for analysis EMSURE® 104944 219
Potassium chloride 99.999 Suprapur® 104938 232
Potassium chloride
Research Article: MCP-1, KC-like and IL-8 as critical mediators of
145 191
SD 44 474 87 48 1362 0 309 231 42
Complicated day 7 (n = 17)
Min-Max 135–2822 110–2184 12–4038 35–3633 425–8818 1–548 29–5548 2–8748 156–7667
Median 254 830 65 84 1911 1 253
Kitalysis™ High-Throughput Screening Platform
excel file.
https://www.sigmaaldrich.com/US/en/deepweb/assets/sigmaaldrich/product/documents/333/219/solvents-weak-base.xlsm
Page 16 of 69
Set-Up Procedure
Preheat a hot plate to 100 °
Mobius®Chrom 20 System User guide
readings
AE203/204
AF45 : 0-3 Au +/-2% FS
OPL: 1 or 2.5 mm
Wavelength: 280 nm
AF46 : 0-2 Au +/-2% FS
OPL: 1 or 2.5 mm
Wavelength: Dual (280/300 nm or
254/280 nm)
In-line
Cytoskeletal Signaling Antibodies, Reagents and Kits
cDNA (dominant
negative)
05-378 anti-RhoGAP,
p190, clone D2D6
06-730 anti-Rho-GDI
05-219 anit-Vav
07-192 Anti-Vav
07-464 anti-Vav3
17-283 Rac
Activation Assay
17-369 Rac2
Activation
inNovations Newsletter #17
GST 20.2 15.2 750 255
TYMV b-gal 16.3 7.6 381 179
TYMV NusA 16.5 10.0 331 208
TYMV GST 16.1 13.3 219 239
1 OD600
2 Pure protein yield expressed as µg/ml culture was determined by BCA assay (BCA Protein
XP725 Antibody List 013M4793
1616 DAXX,Daxx NP_031855.3,NP_001341.1 P y n/d n/d
218 DcR1 D3566 8794 TNFRSF10C NP_003832.2 P y y y
219 DcR2 D3188 8793 TNFRSF10D NP_003831.2 P y n/d n/d
220 DcR3 D1814 8771, 497118 TNFRSF6B,Dcr3 NP_116563.1
3D BIOPRINTING: Printing a Brighter Future
hydroxyapatite, can also be used to make composite GelMA bioinks
with tuned mechanical properties.216–219 In addition, multi-walled
carbon nanotubes (MWCNTs) have also been added to GelMA to
enhance both
3D BIOPRINTING: Printing a Brighter Future
hydroxyapatite, can also be used to make composite GelMA bioinks
with tuned mechanical properties.216–219 In addition, multi-walled
carbon nanotubes (MWCNTs) have also been added to GelMA to
enhance both
XP725 Antibody List
1616 DAXX,Daxx NP_031855.3,NP_001341.1 P y n/d n/d
218 DcR1 D3566 8794 TNFRSF10C NP_003832.2 P y y y
219 DcR2 D3188 8793 TNFRSF10D NP_003831.2 P y n/d n/d
220 DcR3 D1814 8771, 497118 TNFRSF6B,Dcr3 NP_116563.1
Analytical Procedures and Annexes - 1.73028 - ES
plus"
310 Irradiación UV (absorción UV
254 nm) 5)
0,0000 – 3,000 A/cm
0,0000 – 3,000 cm-1
0,00 – 300,0 m-1
Absorción a 254 nm
2611 Iso-α-Ácides 2) 0 – 60 mg/l Absorción UV
2525
Analytical Procedures and Annexes - 1.73027 - ES
plus"
310 Irradiación UV (absorción UV
254 nm) 5)
0,0000 – 3,000 A/cm
0,0000 – 3,000 cm-1
0,00 – 300,0 m-1
Absorción a 254 nm
2611 Iso-α-Ácides 2) 0 – 60 mg/l Absorción UV
2525
User Guide - 1.73017 - Analytical Procedures and Annexes - Spanish
espectral α(254)
0,1 – 250 m-1 Medición a 254 nm
302 Coeficiente de absorción
espectral α(436)
0,1 – 250 m-1 Medición a 436 nm
301 Coeficiente de atenuación
espectral µ(254)
0,1 –
User Guide - 1.73017 - Analytical Procedures and Annexes - Spanish
espectral α(254)
0,1 – 250 m-1 Medición a 254 nm
302 Coeficiente de absorción
espectral α(436)
0,1 – 250 m-1 Medición a 436 nm
301 Coeficiente de atenuación
espectral µ(254)
0,1 –