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Showing 1-30 of 83 results for "222488" within Papers
J M Fujitaki et al.
Journal of biochemical and biophysical methods, 29(2), 143-148 (1994-09-01)
A simple spectrophotometric assay has been developed for determining concentrations of the new cardioprotective agent, acadesine (AICA-riboside), in human plasma. The method is adapted from the Bratton-Marshall (BMA) procedure for detection of primary aromatic amines. The assay was developed to
A C Roy et al.
Electrophoresis, 13(6), 396-397 (1992-06-01)
A sensitive staining method for the detection of oxytocinase (EC activity in electrophoresis gels has been described. The method is based on the enzymatic release of p-nitroaniline (PNA) from two specific synthetic oxytocinase substrates, S-benzyl-L-cysteine-p-nitroanilide (BCN) and L-leucine-p-nitroanilide (LN)
Product Analysis of Starch Active Enzymes by TLC.
Cockburn D and Koropatkin N.
Bio-protocol, 5(20) (2015)
Yolanda Corbett et al.
Microbes and infection, 17(3), 184-194 (2014-12-03)
In malaria, the evidence concerning the nucleotide-binding, oligomerization domain (NOD) 2 (NOD2) receptor is fragmented and the stimuli that might activate NOD2 are not well characterized. We investigated the role of NOD2 in vitro in the response of macrophages to Plasmodium
M Kobayashi et al.
Bioscience, biotechnology, and biochemistry, 61(11), 1836-1839 (1997-12-24)
A convenient detection of sugar chains in Taka-amylase A (TAA) was done by using 40 micrograms of enzyme, where a decrease in the UV absorption of NaIO4 during the periodate oxidation reaction was monitored. The periodate-oxidized sugar chain was labeled
L Jaganathan et al.
BMC biochemistry, 1, 3-3 (2001-03-07)
In vertebrates, two types of cholinesterases exist, acetylcholinesterase and butyrylcholinesterase. The function of acetylcholinesterase is to hydrolyse acetylcholine, thereby terminating the neurotransmission at cholinergic synapse, while the precise physiological function of butyrylcholinesterase has not been identified. The presence of cholinesterases
Kyung Yong Sung et al.
Journal of microbiology and biotechnology, 24(6), 854-861 (2014-03-13)
The diagnosis of Brucella abortus is mainly based on serological methods using antibody against LPS, which has diagnostic problems. Therefore, to solve this problem, we evaluated two proteins of B. abortus, Cu/Zn superoxide dismutase (SodC) and outer membrane proteins 2b
Roman Paduch et al.
Cancer microenvironment : official journal of the International Cancer Microenvironment Society, 4(2), 187-198 (2011-09-13)
Cyclooxygenases (COX), prostaglandin E(2) (PGE(2)) and nitric oxide (NO) are believed to be some of the most important factors related to colon cancer growth and metastasis. In this study, we aimed to investigate the associations between COX-2, PGE(2) and NO
Miruna-Silvia Stan et al.
The FEBS journal, 282(15), 2914-2929 (2015-06-03)
Quantum dots (QDs) are nanocrystalline semiconductor materials that have been tested for biological applications such as cancer therapy, cellular imaging and drug delivery, despite the serious lack of information of their effects on mammalian cells. The present study aimed to
R K Goldman et al.
Analytical biochemistry, 259(1), 98-103 (1998-05-30)
A high-pressure liquid chromatography (HPLC) assay for measuring picomole quantities of nitrosothiol in biological samples was developed. The assay utilizes the catalytic reduction of nitrosothiol by mercuric cation (Hg2+). Released nitrogen oxide reacts with sulfanilamide (SA) and N-(1-napthyl)ethylenediamine (NNED) to
Alex Schudel et al.
Advanced healthcare materials, 4(10), 1484-1490 (2015-05-06)
A synthetic polymer nanoparticle formulation utilizing the physiological nitrosothiol chemistry for nitric oxide delivery is shown. Toxicity of S-nitroso nanoparticles against adult female Brugia malayi worms, which are responsible for lymphatic filariasis, is dependent on nitric oxide release through transnitrosation
Yun Jeong Kim et al.
PloS one, 10(4), e0120840-e0120840 (2015-04-09)
Botulinum neurotoxin type A (BoNT/A) is the most potent protein toxin and causes fatal flaccid muscle paralysis by blocking neurotransmission. Application of BoNT/A has been extended to the fields of therapeutics and biodefense. Nevertheless, the global response of host immune
K K Verma et al.
Talanta, 38(3), 283-289 (1991-03-01)
A general assay procedure for a wide variety of thiols is described. The technique has three steps: (1) formation of S-nitrosothiols with nitrous acid, (2) destruction of the excess of nitrous acid, (3) hydrolysis of the S-nitrosothiols with mercuric ions
Fariheen Aisha Ansari et al.
Toxicology in vitro : an international journal published in association with BIBRA, 29(7), 1878-1886 (2015-08-02)
Nitrite salts are present as contaminants in drinking water and in the food and feed chain. In this work, the effect of sodium nitrite (NaNO2) on human erythrocytes was studied under in vitro conditions. Incubation of erythrocytes with 0.1-10.0 mM
M Kobayashi et al.
Analytical biochemistry, 219(2), 189-194 (1994-06-01)
Fluorescent measurement of the carboxyl group was achieved using water-soluble carbodiimide, EDC (1-ethyl-3-(3- dimethylaminopropyl)carbodiimide). Detection of the active intermediate from the glucuronic acid-EDC reaction mixture using the OPA (o-phthalaldehyde) reagent described in a previous paper was greatly improved to about
Xiaoying Xu et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 980, 20-27 (2015-01-16)
Glutamic acid (Glu) and aspartic acid (Asp), as two important neurotransmitters, have been the focus of increasingly intense research over the past several years. Glu and Asp are present in biological fluids such as serum at trace levels, but complex
Dae-Ryoung Park et al.
PloS one, 10(8), e0134962-e0134962 (2015-08-08)
Oxothiazolidine carboxylic acid is a prodrug of cysteine that acts as an anti-diabetic agent via insulin secretion and the formation of the Ca2+-mobilizing second messenger, cyclic ADP-ribose (cADPR). Here we show that a hybrid compound, arginine thiazolidine carboxylate (ATC), increases
Analytical Chemistry, 55, 1226-1226 (1983)
Patricia Espinosa-Cueto et al.
BMC immunology, 16, 38-38 (2015-06-24)
Tuberculosis (TB) remains a serious human health problem that affects millions of people in the world. Understanding the biology of Mycobacterium tuberculosis (Mtb) is essential for tackling this devastating disease. Mtb possesses a very complex cell envelope containing a variety
Ji Eun Moon et al.
Journal of medicinal food, 18(11), 1223-1230 (2015-10-27)
Rhus verniciflua stokes (RVS) (Anacardiaceae) has been traditionally used as a folk remedy for gastritis, several cancers, and various metabolic diseases. The present study evaluated the anti-inflammatory effect of RVS extract standardized to fustin content using lipopolysaccharide (LPS)-stimulated rats. The
Mayara Klimuk Uchiyama et al.
Toxicological sciences : an official journal of the Society of Toxicology, 142(2), 497-507 (2014-09-28)
Gold nanoparticle (AuNP) bioconjugates have been used as therapeutic and diagnostic tools; however, in vivo biocompatibility and cytotoxicity continue to be two fundamental issues. The effect of AuNPs (20 nm) conjugated with antibody [immunoglobulin G (IgG)], albumin, protein A, PEG4000, and citrate
Bhavana Sethu Lakshmi et al.
Vaccine, 32(30), 3816-3822 (2014-05-13)
Leishmaniasis is a neglected tropical disease caused by Leishmania species. It is a major health concern affecting 88 countries and threatening 350 million people globally. Unfortunately, there are no vaccines and there are limitations associated with the current therapeutic regimens
Basavaraj Hiremath et al.
Acta pharmaceutica (Zagreb, Croatia), 58(3), 275-285 (2008-12-24)
Two spectrophotometric methods for the determination of ceftazidime (CFZM) in either pure form or in its pharmaceutical formulations are described. The first method is based on the reaction of 3-methylbenzothiazolin-2-one hydrazone (MBTH) with ceftazidime in the presence of ferric chloride
Yu-Min He et al.
Journal of natural medicines, 69(3), 366-374 (2015-04-22)
The root of Gentiana crassicaulis has been widely used for the treatment of rheumatism, arthralgia, apoplexy, jaundice and diabetes in traditional medicines, and contains secoiridoid glycosides as the main active ingredients. In the present study, five new secoiridoid glycosides, 6'-O-β-D-xylopyranosylgentiopicroside
Nam Joong Kim et al.
Molecular immunology, 65(1), 17-24 (2015-01-21)
Staphylococcus aureus is a Gram-positive bacterium that causes inflammation at infection sites by inducing various inflammatory mediators such as nitric oxide (NO). To identify the staphylococcal virulence factors contributing to NO production, we compared the ability of ethanol-killed wild-type S.
F Robert-Peillard et al.
Talanta, 88, 230-236 (2012-01-24)
This paper presents a simple, accurate and multi-sample method for the determination of volatile fatty acids (VFAs) thanks to a 96-well microplate technique. A procedure using an activating reagent of the carboxylic function (water-soluble carbodiimide EDC) and a fluorescent amino
Chi-Su Yoon et al.
Molecules (Basel, Switzerland), 23(9) (2018-09-20)
Nardostachys jatamansi contains various types of sesquiterpenoids that may play an important role in the potency of plant's anti-inflammatory effects, depending on their structure. In this study, five new sesquiterpenoids, namely kanshone L (1), kanshone M (2), 7-methoxydesoxo-narchinol (3), kanshone
Hyung-Woo Lim et al.
Brain research bulletin, 112, 25-34 (2015-01-18)
Neuroinflammation is one of the critical pathological mechanisms influencing various neurodegenerative disorders. Most of the neurodegenerative diseases involve over-activation of microglial cells contributing to the demise of neurons. The objective of the current study is to evaluate the anti-inflammatory effect
Pouria Heydarpour et al.
Progress in neuro-psychopharmacology & biological psychiatry, 64, 131-141 (2015-08-14)
Crohn's disease (CD) is associated with increased psychiatric co-morbidities. Nitric oxide (NO) is implicated in inflammation and tissue injury in CD, and it may also play a central role in pathogenesis of the accompanying behavioral despair. This study investigated the
Yun-You Zhou et al.
Guang pu xue yu guang pu fen xi = Guang pu, 25(8), 1318-1321 (2005-12-07)
A simple and sensitive fluorescence quenching method for the determination of trace nitrite was developed. The method is based on the reaction of NED with nitrite in acidic medium to form a new compound, which has weak fluorescence. The linear
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