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Showing 1-30 of 70 results for "240117" within Papers
Guido van Mierlo et al.
Cell reports, 34(5), 108705-108705 (2021-02-04)
Membraneless organelles are liquid condensates, which form through liquid-liquid phase separation. Recent advances show that phase separation is essential for cellular homeostasis by regulating basic cellular processes, including transcription and signal transduction. The reported number of proteins with the capacity
Sreejith J Nair et al.
Nature structural & molecular biology, 26(3), 193-203 (2019-03-06)
A crucial feature of differentiated cells is the rapid activation of enhancer-driven transcriptional programs in response to signals. The potential contributions of physicochemical properties of enhancer assembly in signaling events remain poorly understood. Here we report that in human breast
Daehun Park et al.
Nature communications, 12(1), 263-263 (2021-01-13)
Clusters of tightly packed synaptic vesicles (SVs) are a defining feature of nerve terminals. While SVs are mobile within the clusters, the clusters have no boundaries consistent with a liquid phase. We previously found that purified synapsin, a peripheral SV
Nataliya Shulga et al.
Molecular and cellular biology, 23(2), 534-542 (2003-01-02)
The nuclear pore complex (NPC) is a permeable sieve that can dilate to facilitate the bidirectional translocation of a wide size range of receptor-cargo complexes. The binding of receptors to FG nucleoporin docking sites triggers channel gating by an unknown
Microwave-driven polyol method for preparation of TiO2 nanocrystallites
Yamamoto T, et al.
Chemistry Letters (Jpn), 31(10), 964-965 (2002)
Ashwanth C Francis et al.
Nature communications, 11(1), 3505-3505 (2020-07-16)
The early steps of HIV-1 infection, such as uncoating, reverse transcription, nuclear import, and transport to integration sites are incompletely understood. Here, we imaged nuclear entry and transport of HIV-1 replication complexes in cell lines, primary monocyte-derived macrophages (MDMs) and
W Spahl et al.
Journal of dentistry, 26(2), 137-145 (1998-04-16)
The purpose of our study was to determine the quality and quantity of leachable residual (co)monomers and additives eluted from various commercial dental composite resins after polymerization. Polymerized specimens from four universal hybrid-type composite resins were eluted for 3 days
Yi Lin et al.
Cell, 167(3), 789-802 (2016-10-22)
Two complementary approaches were used in search of the intracellular targets of the toxic PR poly-dipeptide encoded by the repeat sequences expanded in the C9orf72 form of amyotrophic lateral sclerosis. The top categories of PRn-bound proteins include constituents of non-membrane
Archan Chakraborty et al.
Journal of cell science, 133(9) (2020-03-19)
Under metabolic stress, cellular components can assemble into distinct membraneless organelles for adaptation. One such example is cytidine 5'-triphosphate synthase (CTPS, for which there are CTPS1 and CTPS2 forms in mammals), which forms filamentous structures under glutamine deprivation. We have
Thomas R Peskett et al.
Molecular cell, 70(4), 588-601 (2018-05-15)
Huntington's disease is caused by an abnormally long polyglutamine tract in the huntingtin protein. This leads to the generation and deposition of N-terminal exon1 fragments of the protein in intracellular aggregates. We combined electron tomography and quantitative fluorescence microscopy to
Xiao-Man Liu et al.
eLife, 10 (2021-11-13)
Exosomes may mediate cell-to-cell communication by transporting various proteins and nucleic acids to neighboring cells. Some protein and RNA cargoes are significantly enriched in exosomes. How cells efficiently and selectively sort them into exosomes remains incompletely explored. Previously, we reported
M J Politis et al.
Journal of neurocytology, 9(4), 505-516 (1980-08-01)
This study examined the thesis that 2,5-dexanedione (2,5-HD) produces distal (dying-back) axonopathy by direct toxic action on nerve fibers. Single or repeated application of undiluted or 10% 2,5-HD to exposed rat sciatic nerves caused some fibres to develop focal axonal
M A Rahman et al.
Dental materials journal, 14(2), 230-235 (1995-12-01)
The optimum concentration and application time for an experimental dentin primer composed of an aqueous solution of 1,6-hexanediol were determined by measuring the wall-to-wall polymerization contraction gap width of a commercial light-activated resin composite in a cylindrical dentin cavity prepared
Yu-San Yang et al.
Cell, 177(3), 697-710 (2019-04-16)
Yeast ataxin-2, also known as Pbp1 (polyA binding protein-binding protein 1), is an intrinsically disordered protein implicated in stress granule formation, RNA biology, and neurodegenerative disease. To understand the endogenous function of this protein, we identify Pbp1 as a dedicated
Christiane Iserman et al.
Molecular cell, 80(6), 1078-1091 (2020-12-09)
We report that the SARS-CoV-2 nucleocapsid protein (N-protein) undergoes liquid-liquid phase separation (LLPS) with viral RNA. N-protein condenses with specific RNA genomic elements under physiological buffer conditions and condensation is enhanced at human body temperatures (33°C and 37°C) and reduced
D Pandolfi et al.
Nucleosides & nucleotides, 18(9), 2051-2069 (1999-11-05)
Synthetic oligonucleotides are increasingly used because of their potential activity as regulators of gene expression. One of their major drawbacks is instability toward nucleases, in particular exonucleases. In this article, we studied some terminal modifications that can enhance exonuclease resistance
H D Durham et al.
Muscle & nerve, 11(2), 160-165 (1988-02-01)
We reported previously that 2,5-hexanedione (2,5-HD), the neurotoxic metabolite of methyl-n-butylketone (MnBK) and n-hexane, induced aggregation of intermediate filaments of the vimentin type in cultured fibroblasts. To determine if these findings have relevance to the mechanism by which these hexacarbons
Amy R Strom et al.
Nature, 547(7662), 241-245 (2017-06-22)
Constitutive heterochromatin is an important component of eukaryotic genomes that has essential roles in nuclear architecture, DNA repair and genome stability, and silencing of transposon and gene expression. Heterochromatin is highly enriched for repetitive sequences, and is defined epigenetically by
Asmin Tulpule et al.
Cell, 184(10), 2649-2664 (2021-04-14)
Receptor tyrosine kinase (RTK)-mediated activation of downstream effector pathways such as the RAS GTPase/MAP kinase (MAPK) signaling cascade is thought to occur exclusively from lipid membrane compartments in mammalian cells. Here, we uncover a membraneless, protein granule-based subcellular structure that
Marc Boehning et al.
Nature structural & molecular biology, 25(9), 833-840 (2018-08-22)
The carboxy-terminal domain (CTD) of RNA polymerase (Pol) II is an intrinsically disordered low-complexity region that is critical for pre-mRNA transcription and processing. The CTD consists of hepta-amino acid repeats varying in number from 52 in humans to 26 in
Jennifer Tuma et al.
The journal of physical chemistry. B, 111(45), 13101-13106 (2007-10-26)
The solution structure of a synthetic DNA mini-hairpin possessing a stilbenediether linker and three G:C base pairs has been obtained using (1)H NMR spectral data and constrained torsion angle molecular dynamics. Notable features of this structure include a compact hairpin
Steven Boeynaems et al.
Molecular cell, 65(6), 1044-1055 (2017-03-18)
Liquid-liquid phase separation (LLPS) of RNA-binding proteins plays an important role in the formation of multiple membrane-less organelles involved in RNA metabolism, including stress granules. Defects in stress granule homeostasis constitute a cornerstone of ALS/FTLD pathogenesis. Polar residues (tyrosine and
Christiane Iserman et al.
bioRxiv : the preprint server for biology (2020-06-27)
A mechanistic understanding of the SARS-CoV-2 viral replication cycle is essential to develop new therapies for the COVID-19 global health crisis. In this study, we show that the SARS-CoV-2 nucleocapsid protein (N-protein) undergoes liquid-liquid phase separation (LLPS) with the viral
Jason Z Zhang et al.
Cell, 182(6), 1531-1544 (2020-08-28)
The fidelity of intracellular signaling hinges on the organization of dynamic activity architectures. Spatial compartmentation was first proposed over 30 years ago to explain how diverse G protein-coupled receptors achieve specificity despite converging on a ubiquitous messenger, cyclic adenosine monophosphate
C Arunan et al.
Peptides, 21(6), 773-777 (2000-08-26)
A novel 1,6-hexanediol diacrylate cross-linked resin was prepared that was subsequently functionalized by using chloromethyl methyl ether to afford a high-capacity resin. The resin exhibited good swelling and its application in the successful synthesis of a 13-residue peptide corresponding to
Maciej Podgórski
Dental materials : official publication of the Academy of Dental Materials, 26(6), e188-e194 (2010-03-20)
In this study three novel dimethacrylates of different chain lengths having bulky bicycloaliphatic rings were synthesized and proposed as possible dental monomers for dental resin mixtures. The monomers were prepared by the reaction of glycidyl methacrylate with dicarboxylic acid esters
Martina Hallegger et al.
Cell, 184(18), 4680-4696 (2021-08-12)
Mutations causing amyotrophic lateral sclerosis (ALS) often affect the condensation properties of RNA-binding proteins (RBPs). However, the role of RBP condensation in the specificity and function of protein-RNA complexes remains unclear. We created a series of TDP-43 C-terminal domain (CTD)
M Durand et al.
Nucleic acids research, 18(21), 6353-6359 (1990-11-11)
An oligodeoxyribonucleotide, d(GCTCACAAT-X-ATTGTGAGC), where X represents a hexaethylene glycol chain, was studied using circular dichroism spectroscopy. Its conformation and conformational stability were compared to those of compounds where X was replaced by four thymines and to the duplex of same
Olga Vladimirova et al.
PLoS pathogens, 17(1), e1009231-e1009231 (2021-01-21)
Liquid-liquid phase separation (LLPS) can drive formation of diverse and essential macromolecular structures, including those specified by viruses. Kaposi's Sarcoma-Associated Herpesvirus (KSHV) genomes associate with the viral encoded Latency-Associated Nuclear Antigen (LANA) to form stable nuclear bodies (NBs) during latent
C Tani et al.
Dental materials journal, 15(1), 39-44 (1996-06-01)
Physical changes in the surface characteristics of dentin during bonding procedures were evaluated by measuring the contact angles of three standard liquids, to determine the surface free energy, and that of a commercially available dual cured dentin bonding agent dropped
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