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Mid-Infrared-based quantitation of highly modified proteins, labeled peptides and peptidomimetics
quantification of highly modified proteins: glycosylation
Human type I interferon α receptor 1 (IFNAR1) is a 530
amino acid protein with a sequence that contains 35
asparagines and 23 glutamines. The average residue
Outstanding performance Purospher®STAR HPLC and UHPLC columns - EMD
adjusted to pH 1.9 with perchloric acid)
B. Acetonitrile
Flow rate: 0.38 mL/min
Pressure: 530 bar
Gradient: 0 min 17 % Acetonitrile, from 17 – 34 % B
in 16 min, reequilibration with 17 % B
from 16.1
Brochure: Purospher STAR HPLC and UHPLC Columns
adjusted to pH 1.9 with perchloric acid)
B. Acetonitrile
Flow rate: 0.38 mL/min
Pressure: 530 bar
Gradient: 0 min 17 % Acetonitrile, from 17 – 34 % B
in 16 min, reequilibration with 17 % B
Brochure: HPLC Method Development Guide
HR 15 cm x 4.6 mm I.D.; detection: 247 nm; temperature: 40 °C;
flow rate: 1.3 mL/min; mobile phase A: water, mobile phase B: acetonitrile;
gradient conditions: 0 min 45% B, 2.5 min 95% B; sample (in
Chrombook 2015 - The world of chromatography in your hands
perchloric acid)
B: Acetonitrile
Gradient 0 min 17 % B
16 min 17-34 % B
16.1-25 min re-equilibration with 17 % B
Flow rate 0.38 mL/min
Pressure 530 bar
Temperature 40°C
Injection volume 2 µL
Sample
Brochure: The Supelco® HPLC and UHPLC Column Selection Guide
Eluents: A. Water, B. Acetonitrile
UV: 247 nm
Injection volume: 10 µL
Green: Purospher™ STAR RP-18e (5 µm)
LiChroCART® 150-4.6
Gradient: 0 min 45 % B,
from 45 to 95 % B in
Reagents for peptide labeling & biomolecule conjugation
Dirksen & P. E. Dawson (2008) Curr. Opin. Chem. Biol., 18, 760.
16. J. Offer(2010) Biopolymers, 94, 530.
17. T. W. Muir (2003) Ann. Rev. Biochem., 72, 249.
18. R. von Eggelkraut-Gottanka, et. al. (2003)
Cytoskeletal Signaling Antibodies, Reagents and Kits
35
Protein
B-Raf (V599E), active KA 10 µg 14-557
B-Raf (δ;1-415), active KA 10 µg 14-530
Raf-1 (truncated), active KA 10 µg 14-352
Ral
Antibody
anti-RalB 25 H M R WB Pur M IgG
MAPK: Antibodies, Proteins, Kits, siRNA and Reagents
-003601
Protein
B-Raf (V599E), active KA 10 µg 14-557
B-Raf (δ1-415), active KA 10 µg 14-530
Raf-1 (truncated), active KA 10 µg 14-352
35
Ral
RalA and RalB
XP725 Antibody List 013M4793
478104.2 M y n/d n/d
528 Anti Cy3+Cy5 C0992
529 p19INK4d P4354 1032 CDKN2D NP_524145.1 M y n/d n/d
530 p21WAF1/Cip1 P1484 12575, 1026 CDKN1A,Cdkn1a NP_031695.1,NP_510867.1 M y y n/d
531 p300/CBP P2859
XP725 Antibody List
CDKN2A NP_478104.2 M y n/d n/d
528 Anti Cy3+Cy5
529 p19INK4d P4354 1032 CDKN2D NP_524145.1 M y n/d n/d
530 p21WAF1/Cip1 P1484 12575, 1026 CDKN1A,Cdkn1a NP_031695.1,NP_510867.1 M y y n/d
531 p300/CBP P2859
Inhibitor Sourcebook 3rd Edition
reversibly and ATP-competitively inhibits several protein kinases (IC50 = 29 nM, 34
nM, 30 nM, 590 nM and 530 nM for PKCa, PKCß, PKCg, PKCd and PKC; IC50 = 7 nM, 27
nM, 50 nM, 50 nM, 150 nM and