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Showing 1-30 of 82 results for "25059" within Papers
Maryanne F Stock et al.
The Journal of biological chemistry, 278(52), 52315-52322 (2003-10-08)
The kinesin family member BimC has a highly positively charged domain of approximately 70 amino acids at the N terminus of the motor domain. Motor domain constructs of BimC were prepared with and without this extra domain to determine its
Chou-Wei Chang et al.
Journal of virology, 89(3), 1703-1718 (2014-11-21)
BGLF4 kinase, the only Ser/Thr protein kinase encoded by the Epstein-Barr virus (EBV) genome, phosphorylates multiple viral and cellular substrates to optimize the cellular environment for viral DNA replication and the nuclear egress of nucleocapsids. Previously, we found that nuclear
H Shokri
Journal de mycologie medicale, 24(3), 179-184 (2014-04-22)
To investigate the DNA fingerprinting of Candida zeylanoides (C. zeylanoides) strains and the correlation between genotyping and antifungal susceptibility of C. zeylanoides. Paper discs containing nystatin, ketoconazole, fluconazole, Zataria multiflora (Z. multiflora) and Pulicaria gnaphalodes (P. gnaphalodes) essential oils were
In-Sun Choi et al.
Neuroreport, 15(10), 1639-1642 (2004-07-03)
Experimentally induced heterotopia exhibit many of the anatomical features characteristic of cortical malformations in children with early-onset epilepsy. We used extracellular field potential recordings from the dorsal hippocampus of intact adult rats to determine whether the excitability of CA1 pyramidal
Beata Fuchsova et al.
Experimental cell research, 338(2), 170-182 (2015-08-01)
Adenovirus serotypes have been shown to cause drastic changes in nuclear organization, including the transcription machinery, during infection. This ability of adenovirus to subvert transcription in the host cell facilitates viral replication. Because nuclear actin and nuclear myosin I, myosin
Hiroto Ogawa et al.
Biochemical and biophysical research communications, 467(2), 185-190 (2015-10-13)
The spatial dynamics of action potentials, including their propagation and the location of spike initiation zone (SIZ), are crucial for the computation of a single neuron. Compared with mammalian central neurons, the spike dynamics of invertebrate neurons remain relatively unknown.
Blanca V Rodriguez et al.
Analytical biochemistry, 492, 69-75 (2015-09-30)
High-quality chromosomal DNA is a requirement for many biochemical and molecular biological techniques. To isolate cellular DNA, standard protocols typically lyse cells and separate nucleic acids from other biological molecules using a combination of chemical and physical methods. After a
A P M Wolterbeek et al.
Regulatory toxicology and pharmacology : RTP, 40(1), 1-8 (2004-07-22)
Plant lignan 7-hydromatairesinol, a novel precursor of the mammalian lignan enterolactone was evaluated in a prenatal developmental toxicity study conducted in the Wistar rat. Mated female rats were fed diets containing 0, 0.25, 1, and 4% (w/w) of 7-hydroxymatairesinol in
Jun Wei Pek et al.
The Journal of cell biology, 211(2), 243-251 (2015-10-28)
Stable intronic sequence RNAs (sisRNAs) have been found in Xenopus tropicalis, human cell lines, and Epstein-Barr virus; however, the biological significance of sisRNAs remains poorly understood. We identify sisRNAs in Drosophila melanogaster by deep sequencing, reverse transcription polymerase chain reaction
Andreas Gietl et al.
Nucleic acids research, 42(10), 6219-6231 (2014-04-20)
During transcription initiation, the promoter DNA is recognized and bent by the basal transcription factor TATA-binding protein (TBP). Subsequent association of transcription factor B (TFB) with the TBP-DNA complex is followed by the recruitment of the ribonucleic acid polymerase resulting
Ifigenia Geornaras et al.
Food microbiology, 23(8), 762-771 (2006-09-01)
This study evaluated post-processing chemical solutions for their antilisterial effects on commercial smoked sausage formulated with or without 1.5% potassium lactate plus 0.05% sodium diacetate, and contaminated (approximately 3-4 log cfu/cm(2)) with 10-strain composite Listeria monocytogenes inocula prepared under various
Sophie L Woehrer et al.
The EMBO journal, 34(4), 559-577 (2015-01-16)
The loading of small interfering RNAs (siRNAs) and microRNAs into Argonaute proteins is enhanced by Hsp90 and ATP in diverse eukaryotes. However, whether this loading also occurs independently of Hsp90 and ATP remains unclear. We show that the Tetrahymena Hsp90
Hiroe Hayashi et al.
Bioscience, biotechnology, and biochemistry, 78(3), 510-515 (2014-07-19)
We identified and analyzed a DNA region that is required for the stable maintenance of plasmids in the genus Sphingomonas. This DNA fragment, a 244 bp, is localized in the upstream region of the repA gene of low-copy-number small plasmid
Evgenia Karousou et al.
Molecules (Basel, Switzerland), 19(6), 7959-7980 (2014-06-14)
Glycosaminoglycans (GAGs) due to their hydrophilic character and high anionic charge densities play important roles in various (patho)physiological processes. The identification and quantification of GAGs in biological samples and tissues could be useful prognostic and diagnostic tools in pathological conditions.
Carrie Goodson et al.
Eukaryotic cell, 10(12), 1592-1606 (2011-11-01)
Light microscopy and deep-etch electron microscopy were used to visualize triacylglyceride (TAG)-filled lipid bodies (LBs) of the green eukaryotic soil alga Chlamydomonas reinhardtii, a model organism for biodiesel production. Cells growing in nitrogen-replete media contain small cytoplasmic lipid bodies (α-cyto-LBs)
Nils Tore Vethe et al.
Therapeutic drug monitoring, 36(1), 108-118 (2013-09-26)
The development of biomarkers describing the individual responses to the immunosuppressant mycophenolic acid (MPA) has focused on the target enzyme activity [inosine 5'-monophosphate dehydrogenase (IMPDH)]. An extended strategy is to quantify the metabolic consequences of IMPDH inhibition. The aim of
David C Henderson et al.
Molecular biotechnology, 53(2), 109-117 (2012-01-25)
To assay for viruses in plant samples, we required a method for nucleic acid isolation that is rapid, simple, and applicable to the widest possible variety of plants. A protocol for isolation of total nucleic acid (TNA) was developed by
Yiren Xu et al.
mAbs, 7(1), 231-242 (2014-11-27)
Bispecific antibodies have emerged in recent years as a promising field of research for therapies in oncology, inflammable diseases, and infectious diseases. Their capability of dual target recognition allows for novel therapeutic hypothesis to be tested, where traditional mono-specific antibodies
Sovann Kin et al.
Journal of food science, 76(4), S242-S250 (2012-03-16)
Sodium or potassium salts such as lactate and acetate can be used to inhibit the growth of spoilage bacteria and food-borne pathogens, and thereby prolong the shelf-life of refrigerated seafood. However, minimal information is available regarding the combined effects of
Saša Haberl et al.
The Journal of membrane biology, 246(11), 861-867 (2013-07-09)
The use of plasmid DNA (pDNA) as a pharmaceutical tool has increased since it represents a safer vector for gene transfer compared to viral vectors. Different pDNA extraction methods have been described; among them is alkaline lysis, currently the most
Faith A Bazley et al.
Stem cells and development, 24(22), 2634-2648 (2015-07-15)
Primordial germ cells (PGCs) share many properties with embryonic stem cells (ESCs) and innately express several key pluripotency-controlling factors, including OCT4, NANOG, and LIN28. Therefore, PGCs may provide a simple and efficient model for studying somatic cell reprogramming to induced
Nina Grossmann et al.
Nature communications, 5, 5419-5419 (2014-11-08)
The ATP-binding cassette (ABC) transporter associated with antigen processing (TAP) participates in immune surveillance by moving proteasomal products into the endoplasmic reticulum (ER) lumen for major histocompatibility complex class I loading and cell surface presentation to cytotoxic T cells. Here
Chantell Wilson et al.
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, 23(11), 2328-2337 (2014-10-12)
Although colorectal cancer is a disease characterized by sequential accumulation of mutations in epithelial cells, mechanisms leading to genomic vulnerability contributing to tumor initiation remain undefined. GUCY2C has emerged as an intestine-specific tumor suppressor controlling epithelial homeostasis through circuits canonically
Petra van der Lelij et al.
The EMBO journal, 33(22), 2643-2658 (2014-09-27)
Although splicing is essential for the expression of most eukaryotic genes, inactivation of splicing factors causes specific defects in mitosis. The molecular cause of this defect is unknown. Here, we show that the spliceosome subunits SNW1 and PRPF8 are essential
Song P Seto et al.
Bioconjugate chemistry, 26(2), 286-293 (2015-01-27)
Bone morphogenetic protein-2 (BMP-2) plays an important role in bone and cartilage formation and is of interest in regenerative medicine. Heparin can interact electrostatically with BMP-2 and thus has been explored for controlled release and potential stabilization of this growth
X L Jin et al.
Reproduction (Cambridge, England), 148(2), 147-157 (2014-06-26)
The co-expression of the CREB and ATF1 transcription factors is required for the development of preimplantation embryos. Embryotropin-mediated, calcium/calmodulin-dependent signalling activates CREB-induced transcription in the two-cell embryo, but the regulation of ATF1 in the embryo is not known. This study
Florence Teulé et al.
Nature protocols, 4(3), 341-355 (2009-02-21)
The extreme strength and elasticity of spider silks originate from the modular nature of their repetitive proteins. To exploit such materials and mimic spider silks, comprehensive strategies to produce and spin recombinant fibrous proteins are necessary. This protocol describes silk
Ya-Fang Huang et al.
Antioxidants & redox signaling, 23(12), 973-984 (2015-04-14)
To elucidate the role of reactive oxygen species (ROS) in arthritis and to identify targets of arthritis treatment in conditions with different levels of oxidant stress. Through establishing an arthritis model by injecting arthritogenic serum into wild-type and NADPH oxidase
Alexander G Myasnikov et al.
Nature communications, 5, 5294-5294 (2014-11-08)
During protein synthesis, several ribosomes bind to a single messenger RNA (mRNA) forming large macromolecular assemblies called polyribosomes. Here we report the detailed molecular structure of a 100 MDa eukaryotic poly-ribosome complex derived from cryo electron tomography, sub-tomogram averaging and pseudo-atomic
Guobin Shan et al.
Journal of environmental sciences (China), 20(1), 80-87 (2008-06-25)
Purification of the total DNA extracted from activated sludge samples was studied. The effects of extraction buffers and lysis treatments (lysozyme, sodium dodecyl sulfate (SDS), sonication, mechanical mill and thermal shock) on yield and purity of the total DNA extracted
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