27532-96-3

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Glycogen Synthase Kinase 3 (GSK-3) Overview

Glycogen synthase kinase 3 (GSK-3) is a highly conserved family of serine/threonine kinases for over 100 proteins in many pathways. See a list of accepted GSK-3 modulators and related products.

Downloadable 96 Well Plate Templates

We offer downloadable and printable 24, 48 & 96 well plate templates in PDF, JPG, PNG and SVG formats for design experiments performed in a well plate

Supel™ BioSPME 96-Pin Devices

Supel™ BioSPME 96-Pin devices enhance speed and simplicity for plasma protein binding studies and LCMS analyses.

Custom SPE Tubes & 96-Well Plates

Solid-phase extraction (SPE) offers rapid sample preparation and purification for chromatographic analysis, customizable to analytical needs.

Drug transport assays in a 96-well system: reproducibility and correlation to human absorption

Application note on Drug transport assays in a 96-well system using Millicell-96 System from Millipore.

The 3'/5' Assay for Analysis of RNA Integrity Protocol

The 3’/5’ integrity assay identifies RNA degradation, crucial for large sample sets or less detectable degradation.

GC Analysis of Omega 3 in Fish Oil and Salmon

Analyze omega 3 fatty acid profiles from fish oil capsules and salmon using GC columns for confirmatory analysis of lipid composition.

5'/3' RACE Kit, 2nd Generation Troubleshooting

In addition to the troubleshooting provided in the product manual, most probably the efficiency of the tailing reaction performed by Terminal Transferase could be impaired. This could occur due to several reasons (which will not only affect the control reaction

Diethanolamine Assay for the Enzymatic Assay of Alkaline Phosphatase (EC 3.1.3.1)

Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)

HMG-CoA Reductase (HMGR) Assay Kit

3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) is a transmembrane glycoprotein, located on the endoplasmic reticulum.

Standard Methods 6040D: GC Analysis of Geosmin and 2-MIB on SLB®-5ms after SPME using 50/30 µm DVB/Carboxen/PDMS Fiber

Separation of 2-Isopropyl-3-methoxypyrazine 20 ppt; 2-Isobutyl-3-methoxypyrazine; 2-Methylisoborneol; (±)-Geosmin 10 ppt

GenElute™ 96 Well Tissue Genomic DNA Purification Kit Protocol

This protocol is designed to allow for high throughput purification of pure genomic DNA from a variety of cultured cells and tissues using the GenElute 96 Well Tissue Genomic DNA Purification Kit.

Cholesterol Biosynthesis

Cholesterol biosynthesis starts in the hepatic endoplasmic reticulum with acetyl-CoA, yielding 3-hydroxy-3-methylglutaryl-CoA via HMG-CoA synthase.

Cultrex ® 3-D Culture Matrix™ Laminin I Protocol

3-D Culture Matrix™ Mouse Laminin I may be used as a gel or as a media additive to study cellular growth and differentiation in three dimensions in vitro.

MilliporeSigma Partners with Promega to Advance 3-D Cell Drug Discovery Technologies

MilliporeSigma and Promega will co-develop novel technologies that advance drug discovery.

Microscopic Examination of Samples

This protocol covers 3 modes for the microscopic examination of cell samples.

MultiScreen® Solid Bottom Plates

96-well and 384-well microplates for biochemical assays, ELISA, high throughput screening, and compound storage.

Procedure for Purifying and Concentrating DNA using illustra™ GFX 96 PCR Purification Kit

This page shows the procedure for purifying and concentrating DNA using illustra™ GFX 96 PCR Purification Kit from Cytiva.

Graphene and New Monoatomic Materials: Using 2-Dimensional Nanosheets in a 3-Dimensional World

Cultrex® 3-D Culture Matrix™ Rat Collagen I Protocol

3-D Culture Matrix™ Collagen I facilitates cellular growth and differentiation in vitro, offering versatile applications in biomedical research.

What You Need to Know About the New ISO 16140-3 Microbiological Method Verification

This on-demand webinar introduces the new EN ISO 16140-3 on the verification of methods in food microbiology.

Troubleshooting first-dimension IEF: Ettan™ IPGphor™ 3 Isoelectric Focusing System

Troubleshooting first-dimension isoelectric focusing with Ettan IPGphor 3 Isoelectric Focusing System from Cytiva.

Cell Viability and Proliferation XTT Assay Protocol Guide

Perform colorimetric assays for nonradioactive quantification of cellular proliferation, viability, and cytotoxicity for adherent or suspension cells cultured in 96-well microplates.

Corning® Spheroid Microplates

Understand how Corning® Spheroid Microplates can be used for generating and culturing tumor spheroids in a 96-well spheroid microplate format.

384- vs. 96-Well Multiplex Analysis of Low-Level Cytokines | MILLIPLEX^® Assays

High-sensitivity multiplex panels allow researchers to detect low-level cytokines and study multiple cytokines at once to dive deeper into low-level inflammation processes. See a performance comparison of the 384-well assay and the 96-well assay formats for the MILLIPLEX® Human High

Coating Tissue Culture Plates with Vitronectin

Vitronectin can support cell adhesion through binding to various integrins and other proteoglycans. This protocol is for coating a 96-well plate and other surfaces.

Differentiation of Primary Human Osteoblasts

Cell culture protocol used to differentiate primary human osteoblasts into bone cells in 2-3 weeks.

Enzymatic Assay of Peroxidase (EC 1.11.1.7) 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a Substrate

To standardize a procedure for the assay of Peroxidase using 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a substrate.

Guidelines for Bioassays on MultiScreen Filter Plates: Protein Binding and Cell Loading

Guidelines for various bioassays on the MultiScreen system, which makes it possible to carry out an assay from sample incubation to scintillation counting in a 96-well plate.

Enrichment of Phospholipids in Biological Samples Using HybridSPE-PL

A simple method to enrich phospholipids from plasma samples, involving a HybridSPE-PPT 96-well plate that both retains phospholipids and removes precipitated proteins.

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