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Showing 1-30 of 564 results for "277-385-9" within Site Content

X-tremeGENE™ 9 DNA Transfection Reagent Protocol

Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).

CRISPR Cas 9 Nuclease RNA-guided Genome Editing

Learn about CRISPR Cas9, what it is and how it works. CRISPR is a new, affordable genome editing tool enabling access to genome editing for all.

MilliporeSigma Expands in Gillingham, UK, Boosts Distribution Capabilities

MilliporeSigma today announced the completion of a £9 million expansion to its Gillingham distribution center.

Accelerated mAb Development Program – From Transfection to GMP Drug Substance in 9 Months

The accelerated mAb development program delivers GMP drug substance nine months from transfection. We designed the mAbExpress™ program to be fast, cost effective, and appropriate for the early clinical phase of your program, with regulatory support throughout.

How to Boost AAV Titer with Feed Supplementation in both HEK293 and Sf-9 Platforms

In this webinar, you will learn wow to boost AAV titers in HEK293 and Sf-9 cell lines as well as getting some insider tips on volume and timing optimization.

Analysis of 17 Cannabinoids in Hemp and Cannabis

HPLC separation of 17 important cannabinoids including CBD, delta 9 THC and THCA. Read the application note.

Determination of Phosphorus Total in Meat and Meat Products

Colorimetric total phosphorus analysis method for meat and meat products as per German Food and Feed Code §64 LFGB 06.00-9.

Solving Aspartimide Formation in Fmoc SPPS with Fmoc-Asp(OBno)-OH

Aspartimide formation 1,2 is caused by repeated exposure of aspartic acid-containing sequences to bases like piperidine and can result ultimately in the generation of 9 different by-products.

Analysis of Antioxidants in Bakery Products

HPLC-UV method detects 9 antioxidants like propyl gallate in food using a Discovery®</sup HS C18 column for excellent peak shapes.

Pressure-Temperature Calculator for Solvents

Use the calculator to determine the boiling point or pressure of solvents based on the Antoine Equation and see solvent attributes. View a list of products based on the solvent CAS number.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

Sample Preparation for Chromatographic Purification

This page discusses various aspects of sample preparation for chromatographic purification.

Sensor Dyes

Nitric oxide (NO) as a signal transporter in neurons, endothelial cells and in the immune system.

Epigenetics Overview

Epigenetics is a term coined to describe changes that are not mutation based but can still be passed on from generation to generation. Genes that are activated or repressed without any change in DNA sequence are epigenetically controlled. Epigenetic modifications

Chitosan-based Biomaterials

Chitin, a natural polysaccharide, is the second most abundant natural biopolymer in the world, after cellulose.

Self-Propagating Reactions Induced by Mechanical Alloying

An article concerning self-propagating reactions induced by mechanical alloying, presented by Sigma-Aldrich.com.

Stripping and Reprobing Western Blotting Membranes

This page shows and discusses three protocols for stripping and reprobing a western blot membrane.

DNA Damage and Repair

DNA damage and repair mechanism is vital for maintaining DNA integrity. Damage to cellular DNA is involved in mutagenesis, the development of cancer among others.

Revolution in Plastic Recycling: Laser Marking & Digital Product Passports Enable True Circular Economy

An industry-wide project demonstrates how packaging can become smarter, more sustainable, and fully traceable. Plastics are a blessing—and a global problem. Lightweight, durable, and versatile. Yet these very properties make plastics a challenge worldwide. Over 400 million tons are produced

AOAC Method 2011.11: UHPLC/MS/MS Analysis of Vitamin D2 and Vitamin D3 on Titan™ C18

Separation of Ergocalciferol (D2), analytical standard; Cholecalciferol (D3), analytical standard

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

NMR Deuterated Solvent Properties Reference Chart

Use this reference table to find the coupling values and chemical shifts of our NMR (deuterated) solvents. Melting and boiling points, molecular weight, density, and CAS number are also listed.

Dietary Antioxidants

Antioxidants protect biological systems from oxidative damage produced by oxygen-containing free radicals and from redoxactive transition metal ions such as iron, copper, and cadmium.

Isotopic Labeling for NMR Spectroscopy of Biological Solids

Isotopic Labeling for NMR Spectroscopy of Biological Solids;

Peptide Modifications: N-Terminal, Internal, and C-Terminal

Silylation of Non-Steroidal Anti-Inflammatory Drugs

Results of a study involving the ability few Fluka silylating reagents to form GC-MS-compatible trimethylsilylmethyl derivatives of NSAIDs

Polymersomes for Drug Delivery

The development of drugs that target specific locations within the human body remains one of the greatest challenges in biomedicine today.

Recent Developments in Silicon Anode Materials for High Performance Lithium-Ion Batteries

Recent demand for electric and hybrid vehicles, coupled with a reduction in prices, has caused lithium-ion batteries (LIBs) to become an increasingly popular form of rechargeable battery technology.

Graphene-Based Composites and their Unique Renewable Energy Applications

Graphene is a unique two-dimensional (2D) structure of monolayer carbon atoms packed into a dense honeycomb crystal that has attracted great interest due to its diverse and fascinating properties.

Polyelectrolyte Multilayer Films and Membrane Functionalization

We present an article that discusses two applications in particular; first, using these layers as polyelectrolyte membranes to control permeability.

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