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3.2.1.31
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(G7396) - Enzyme Assay
01/29/98
SIGMA QUALITY CONTROL TEST PROCEDURE
Enzymatic Assay of ββββ-GLUCURONIDASE
(EC 3.2.1.31)
from E. coli
PRINCIPLE:
PheP-Gluc + H2O β
-Glucuronidase> D-Glucuronate + Phenolphthalein
Data Sheet - G1512
from Helix pomatia
Product Number G 1512
Storage Temperature –20 °C
CAS# 9001-45-0
EC 3.2.1.31
Synonyms: β-D-Glucuronide glucuronosohydrolase
Product Description
Glucuronidation, conjugation
Data Sheet - G0876
H-2
from Helix pomatia
Catalog Number G0876
Storage Temperature 2–8 °C
CAS RN 9001-45-0
EC 3.2.1.31
Synonym: β-D-Glucuronide glucuronosohydrolase
Product Description
Glucuronidation, conjugation
Data Sheet - G8885
H-3
from Helix pomatia
Catalog Number G8885
Storage Temperature 2–8 °C
CAS RN 9001-45-0
EC 3.2.1.31
Synonyms: β-D-Glucuronide glucuronosohydrolase
Product Description
Glucuronidation, conjugation
Data Sheet - G0751
from Helix pomatia
Product Number G 0751
Storage Temperature –20 °C
CAS# 9001-45-0
EC 3.2.1.31
Synonyms: β-D-Glucuronide glucuronosohydrolase
Product Description
Glucuronidation, conjugation
Data Sheet - G8420
Escherichia coli BL21
Catalog Number G8420
Storage Temperature –20 °C
CAS RN 9001-45-0
EC 3.2.1.31
Synonyms: β-D-Glucuronide glucuronosohydrolase;
GUS
Product Description
Glucuronidation, conjugation
Data Sheet - G7396
from Escherichia coli
Product Number G7396
Storage Temperature –20 °C
CAS# 9001-45-0
EC 3.2.1.31
Synonyms: β-D-Glucuronide glucuronosohydrolase;
GUS
Product Description
Glucuronidation, conjugation
Data Sheet - G8132
vulgata (keyhole limpet)
Catalog Number G8132
Storage Temperature –20 °C
CAS RN 9001-45-0
EC 3.2.1.31
Synonym: β-D-Glucuronide glucuronosohydrolase
Product Description
Glucuronidation, conjugation
Data Sheet - G2174
limpets (Patella vulgata)
Catalog Number G2174
Storage Temperature 2–8 °C
CAS RN 9001-45-0
EC 3.2.1.31
Synonym: β-D-Glucuronide glucuronosohydrolase
Product Description
Glucuronidation, conjugation
Data Sheet - G7770
from Helix pomatia
Product Number G7770
Storage Temperature 2–8 °C
CAS# 9001-45-0
EC 3.2.1.31
Synonyms: β-D-Glucuronide glucuronosohydrolase
Product Description
Glucuronidation, conjugation
Data Sheet - SRE0095
proprietary
host
Catalog Number SRE0095
Storage Temperature 2–8 C
CAS RN 9001-45-0
EC 3.2.1.31
Synonym: -D-Glucuronide glucuronosohydrolase
Product Description
Glucuronidation (conjugation
Product Information Sheet - G7896
Bacterial
from Escherichia coli
Sigma Prod. No. G7896
CAS: 9001-45-0
ENZYME COMMISSION NUMBER: 3.2.1.31
SYNONYMS: β-D-Glucuronide glucuronosohydrolase; GUS
PHYSICAL DESCRIPTION:
Appearance: White
Data Sheet - G7646
from Escherichia coli
Product Number G 7646
Storage Temperature –20 °C
CAS# 9001-45-0
EC 3.2.1.31
Synonyms: β-D-Glucuronide glucuronosohydrolase;
GUS
Product Description
Glucuronidation, conjugation
Product information sheet
overexpressing Escherichia coli BL21
G8295
Storage Temperature -20 °C
CAS RN 9001-45-0
EC 3.2.1.31
Synonyms: β-D-Glucuronide glucuronosohydrolase; GUS
Product Description
Glucuronidation
Data Sheet-SRE0093
Product Description
CAS Registry Number: 9001-45-0
Enzyme Commission (EC) Number: 3.2.1.31
Synonyms: β-D-Glucuronide glucuronosohydrolase
Glucuronidation (conjugation with glucuronic
Data Sheet - G7017
Product Description
CAS Registry Number: 9001-45-0
Enzyme Commission (EC) Number: 3.2.1.31
Synonyms: β-D-Glucuronide glucuronosohydrolase
Glucuronidation, or conjugation with glucuronic
Product information sheet -SRE0103
glucuronidase
Pre-buffered, automation-ready hydrolysis mix, 6-12 Reactions/mL
SRE0103
EC Number: 3.2.1.31
Product Description
Most drugs, hormones and xenobiotics are
metabolized in humans to hydrophilic
DataSheet - SRE0095
Product Description
CAS Registry Number: 9001-45-0
Enzyme Commission (EC) Number: 3.2.1.31
Synonyms: β-D-Glucuronide glucuronosohydrolase
Glucuronidation (conjugation with glucuronic
DataSheet - G7396
Product Description
CAS Registry Number: 9001-45-0
Enzyme Commission (EC) Number: 3.2.1.31
Synonyms: β-D-Glucuronide glucuronosohydrolase
Molecular Weight: ~290 kDa (tetramer),1 68,259
DataSheet - G8132
Product Description
CAS Registry Number: 9001-45-0
Enzyme Commission (EC) Number: 3.2.1.31
Synonyms: β-D-Glucuronide glucuronosohydrolase
Glucuronidation, or conjugation with glucuronic
DataSheet - G0876
Product Description
CAS Registry Number: 9001-45-0
Enzyme Commission (EC) Number: 3.2.1.31
Synonyms: β-D-Glucuronide glucuronosohydrolase
Glucuronidation, or conjugation with glucuronic
DataSheet - G0751
Product Description
CAS Registry Number: 9001-45-0
Enzyme Commission (EC) Number: 3.2.1.31
Synonyms: β-D-Glucuronide glucuronosohydrolase
Glucuronidation, or conjugation with glucuronic
Product Information Sheet-bgals-ro
: 08 β-Glucuronidase from E. coli K 12
6 sigma-aldrich.com
2.3. Parameters
EC-Number
EC 3.2.1.31
pH Optimum
pH 6.0 to 6.5; activity is reduced above or below this range.
Specific Activity
Product Information Sheet-bgala-ro
dichloromethane.
2.3. Parameters
EC-Number
β-D-Glucuronoside glucuronosohydrolase: EC 3.2.1.31
Arylsulfate sulfohydrolase: EC 3.1.6.1
Inhibition
β-Glucuronidase activity is inhibited by: