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Instruction Manual for EZ-Zyme Chromatin Prep Kit
17-375-manual
Instruction Manual
for
EZ-Zyme™ Chromatin Prep Kit
Catalog # 17‐375
Sufficient reagents for 22 enzymatic chromatin preparations per kit.
Contents Page
I.
Product Information Sheet - CS0006
CS0006pis Rev 09/22
2
Equipment Required (Not
User Guide-Nonsterile 33 mm Millex® Syringe Filters
Results,
PPB*
375-22-4 Perfluorobutanoic acid PFBA 0.0005 BRL
2706-90-3 Perfluoropentanoic acid PFPeA 0.0005 BRL
307-24-4 Perfluorohexanoic acid PFHxA 0.0005 BRL
375-85-9 Perfluoroheptanoic
User Guide-Nonsterile 33 mm Millex® Syringe Filters
Results,
PPB*
375-22-4 Perfluorobutanoic acid PFBA 0.0005 BRL
2706-90-3 Perfluoropentanoic acid PFPeA 0.0005 BRL
307-24-4 Perfluorohexanoic acid PFHxA 0.0005 BRL
375-85-9 Perfluoroheptanoic
Comparative Study - ReadyStream® Footprint Analysis vs others
System with Dilutor
-20% -22% -26% -31%
1875
1226
707 620
273
0
500
1000
1500
2000
in
s
ec
on
ds
Total processing time (sec) per 375 g
TB071VM pET-26b(+) Vector Map
Tsp45I 7 1295 2123 2654 2867 2962
4564 5291
Tsp509I 22
Tth111I 1 2960
Tth111II 8 953 1646 2676 3805 3812
3844 4253 4380
UbaJI 18
VspI 6 207 375 1799 1858 4616
4805
XbaI 1 326
XcmI 3 970 1486 1504
MAK094 - Technical Bulletin
each sample to the 96-well plate.
5. Measure the absorbance at 375 nm
(A375).
Bulletin - CDA20
ml of sample in equation / 10 ml of sample in assay
F = Assay Dilution Factor = (375 ml sample + 5 mi NaOH) + 375 ml sample) = 1.013
Sigma Tech. Bulletin No. CDB-1 (8-96)
User Guide (DFU) - 71008BC
1 °C. Incubate 50 – 375
g samples with pre-warmed media (37 ± 1 °C) for 18 – 29 h at 37 ± 1 °C.
PROCEED TO D. SAMPLE PROCESSING PROTOCOL
User Guide 71038 – Cronobacter Tq II AOAC – EN
g samples for 20 – 28 h at 36 °C. Incubate 26 – 375 g samples for 24 – 32 h at 36 °C.
d. Transfer enriched samples to BHI (Appendix B) broth for 2 – 4 h
User Guide 71008 – Salmonella TQ - AFNOR - MICROVAL - EN
For infant formula without probiotics: For 10–375 g sample size, add to 1:10 sample-to-media
ratio of BPW. For infant cereals (without probiotics): For 10–375 g sample size, add to 1:10 sample-
to-media
User Guide 71008 - Salmonella TQ - AFNOR - MICROVAL - EN
1 °C. Incubate 50 – 375
g samples with pre‐warmed media (37 ± 1 °C) for 18 – 29 h at 37 ± 1 °C.
PROCEED TO D. SAMPLE PROCESSING PROTOCOL
User Guide (DFU) - 71008BC
1 °C. Incubate 50 – 375
g samples with pre-warmed media (37 ± 1 °C) for 18 – 29 h at 37 ± 1 °C.
PROCEED TO D. SAMPLE PROCESSING PROTOCOL
RABBIT ANTI-GABA TRANSPORTER-2 (GAT-2)
Comp. Neurology (1996) 375:212-224.
2) Neuroscience Letters (1999) 266:117-120.
3) J. Comp. Neurology (1999) 409:482-494.
4) Calcagnotto, ME et al., J Neuroscience (2002) 22:7596-7605.
Important
Product Information Sheet-udghl-ro
Storage Buffer: 20 mM Tris-HCl, pH 8.0 (+4°C),
0.1 mM EDTA, 100 mM KCl, 1 mM DTT,
50% glycerol (v/v), 0.05% Tween 20 (v/v).
11 775 367 001 1 vial,
100 µl
11 775 375 001 1 vial
Application Note: Determination of PFAS in Drinking Water by LC-MS/MS according to GB 5750.8-2024
PFBA 375-22-4
2 Perfluoro-3-methoxypropanoic acid PFMPA 377-73-1
3* Perfluorovaleric acid PFPeA 2706-90-3
4* Perfluorobutanesulfonic acid PFBS 375-73-5
5 2,2,3,3,4,4-Hexafluoro-4-(trifluoromethoxy
RABBIT ANTI-GABA TRANSPORTER-1 (GAT-1)
(1996) 375:212-224.
4) J. Neuroscience (1997) 17:6264-6276.
5) J. Comp. Neurology (1998) 396:51-63.
6) NeuroReport (1998) 9:467-470.
7) Calcagnotto, ME et al., J Neuroscience (2002) 22:7596-7605
Product Information Sheet -C6019
in
vitro antifungal activity against clinical yeast
isolates. Int. J. Antimicrob. Agents, 20(5),
375-379 (2002).
8. McLean, K. J., et al., Azole antifungals are potent
inhibitors of cytochrome P450
RABBIT ANTI-GABA TRANSPORTER-3 (GAT-3)
) J. Comp. Neurology (1996) 367:595-606.
2) J. Comp. Neurology (1996) 375:212-224.
3) J. Neuroscience (1996) 16:6255-6264.
4) J. Neuroscience (1997) 17:6264-6276.
5) Neuroscience Letters (1999)
Product Information Sheet - D1529
(2017).
10. Murdock, A.L. et al., Arch. Biochem. Biophys.,
114(2), 375-390 (1966).
11. Smookler, David, "The Role of TIMP3 in Models of
Inflammation and Immunity". University of
Toronto, Ph.D
Datasheet - M0691
Biochem. Biophys. Acta, 1496, 3-22
(2000).
2. Hasson, T., et al., J. Cell Biol., 127, 425-440
(1994).
3. Buss, F., et al., J. Cell Biol., 143, 1535-1545
(1998).
4. Wells, A.L. et
User_Guide
Homogenize or mix sample and incubate for 6–24 h at 41.5 °C ± 1 °C.
2. Raw beef meat up to 375 g – For 375 g test portion, add sample into 1500 mL pre-warmed (41.5
Data Sheet - E6404
. Immunother., 23, 519-527 (2000).
4. Schwarz, M., et al., EMAP II: a modulator of
neovascularization in the developing lung. Am. J.
Physiol., 276, L365-375 (1999).
5. Behrensdorf, H. A., et al
Product Information Sheet - H7133
°C
Product Description
Molecular Formula: C4HF7O2
Molecular Weight: 214.0
CAS Number: 375-22-4
Boiling Point: 120 °C
Density: 1.645 g/ml
Molarity: 7.69 M (based on density)
pKa: approximately
Anti-CRMP1 (C2868)
(2000).
4. Deo, R.C., et al., EMBO J., 23, 9-22 (2004).
5. Yamashita, N., et al., J. Neurosci., 26, 13357-
13362 (2006).
6. Chang, C.C., et al., J. Natl. Cancer Inst., 96, 364-
375 (2004).
ER
Data Sheet - C8241
Reserved.
C8241dat Rev 03/22 NDH,PHC,GCY,MAM
2
User Guide 71015 - MPX TOP 7 STEC - AFNOR - EN
mEHEC® media.
For 375 g test portion, weigh into 1,500 mL pre-warmed (41.5 °C) mEHEC® media. Homogenize or mix
sample for 2 min. Incubate 25 g samples for 8 – 16 h at 41.5 °C. Incubate 375 g samples
Anti-CRMP-1 (N-terminal) (C4118)
(2000).
4. Deo, R.C., et al., EMBO J., 23, 9-22 (2004).
5. Yamashita, N., et al., J. Neurosci., 26, 13357-
13362 (2006).
6. Chang, C.C., et al., J. Natl. Cancer Inst., 96, 364-
375 (2004).
ER
Product Information Sheet - NA9604
W0263 1 x 375 ml
Wash Solution 2 W4639 1 x 75 ml
Elution Solution E7777 2 x 115 ml
Filter Plate F3555 4
Binding Plate B6686 4
Elution Plate (Corning
Costar cell culture plate)
CLS3790 4
AlumaSeal
User Guide 71015BC
media.
For 375 g test portion, weigh into 1,500 mL pre-warmed (41.5 °C) mEHEC® media. Homogenize or mix
sample for 2 min. Incubate 25 g samples for 8 – 16 h at 41.5 °C. Incubate 375 g samples for
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