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Instruction Manual for EZ-Zyme Chromatin Prep Kit
17-375-manual
Instruction Manual
for
EZ-Zyme™ Chromatin Prep Kit
Catalog # 17‐375
Sufficient reagents for 22 enzymatic chromatin preparations per kit.
Contents Page
I.
Product Information Sheet - CS0006
CS0006pis Rev 09/22
2
Equipment Required (Not
User Guide-Nonsterile 33 mm Millex® Syringe Filters
Results,
PPB*
375-22-4 Perfluorobutanoic acid PFBA 0.0005 BRL
2706-90-3 Perfluoropentanoic acid PFPeA 0.0005 BRL
307-24-4 Perfluorohexanoic acid PFHxA 0.0005 BRL
375-85-9 Perfluoroheptanoic
Comparative Study - ReadyStream® Footprint Analysis vs others
System with Dilutor
-20% -22% -26% -31%
1875
1226
707 620
273
0
500
1000
1500
2000
in
s
ec
on
ds
Total processing time (sec) per 375 g
MAK094 - Technical Bulletin
each sample to the 96-well plate.
5. Measure the absorbance at 375 nm
(A375).
Bulletin - CDA20
ml of sample in equation / 10 ml of sample in assay
F = Assay Dilution Factor = (375 ml sample + 5 mi NaOH) + 375 ml sample) = 1.013
Sigma Tech. Bulletin No. CDB-1 (8-96)
User Guide (DFU) - 71008BC
1 °C. Incubate 50 – 375
g samples with pre-warmed media (37 ± 1 °C) for 18 – 29 h at 37 ± 1 °C.
PROCEED TO D. SAMPLE PROCESSING PROTOCOL
User Guide 71008 - Salmonella TQ - AFNOR - MICROVAL - EN
1 °C. Incubate 50 – 375
g samples with pre‐warmed media (37 ± 1 °C) for 18 – 29 h at 37 ± 1 °C.
PROCEED TO D. SAMPLE PROCESSING PROTOCOL
User Guide (DFU) - 71008BC
1 °C. Incubate 50 – 375
g samples with pre-warmed media (37 ± 1 °C) for 18 – 29 h at 37 ± 1 °C.
PROCEED TO D. SAMPLE PROCESSING PROTOCOL
RABBIT ANTI-GABA TRANSPORTER-2 (GAT-2)
Comp. Neurology (1996) 375:212-224.
2) Neuroscience Letters (1999) 266:117-120.
3) J. Comp. Neurology (1999) 409:482-494.
4) Calcagnotto, ME et al., J Neuroscience (2002) 22:7596-7605.
Important
Product Information Sheet-udghl-ro
Storage Buffer: 20 mM Tris-HCl, pH 8.0 (+4°C),
0.1 mM EDTA, 100 mM KCl, 1 mM DTT,
50% glycerol (v/v), 0.05% Tween 20 (v/v).
11 775 367 001 1 vial,
100 µl
11 775 375 001 1 vial
RABBIT ANTI-GABA TRANSPORTER-3 (GAT-3)
) J. Comp. Neurology (1996) 367:595-606.
2) J. Comp. Neurology (1996) 375:212-224.
3) J. Neuroscience (1996) 16:6255-6264.
4) J. Neuroscience (1997) 17:6264-6276.
5) Neuroscience Letters (1999)
RABBIT ANTI-GABA TRANSPORTER-1 (GAT-1)
(1996) 375:212-224.
4) J. Neuroscience (1997) 17:6264-6276.
5) J. Comp. Neurology (1998) 396:51-63.
6) NeuroReport (1998) 9:467-470.
7) Calcagnotto, ME et al., J Neuroscience (2002) 22:7596-7605
Product Information Sheet -C6019
in
vitro antifungal activity against clinical yeast
isolates. Int. J. Antimicrob. Agents, 20(5),
375-379 (2002).
8. McLean, K. J., et al., Azole antifungals are potent
inhibitors of cytochrome P450
Datasheet - M0691
Biochem. Biophys. Acta, 1496, 3-22
(2000).
2. Hasson, T., et al., J. Cell Biol., 127, 425-440
(1994).
3. Buss, F., et al., J. Cell Biol., 143, 1535-1545
(1998).
4. Wells, A.L. et
Product Information Sheet - D1529
(2017).
10. Murdock, A.L. et al., Arch. Biochem. Biophys.,
114(2), 375-390 (1966).
11. Smookler, David, "The Role of TIMP3 in Models of
Inflammation and Immunity". University of
Toronto, Ph.D
Data Sheet - E6404
. Immunother., 23, 519-527 (2000).
4. Schwarz, M., et al., EMAP II: a modulator of
neovascularization in the developing lung. Am. J.
Physiol., 276, L365-375 (1999).
5. Behrensdorf, H. A., et al
Product Information Sheet - H7133
°C
Product Description
Molecular Formula: C4HF7O2
Molecular Weight: 214.0
CAS Number: 375-22-4
Boiling Point: 120 °C
Density: 1.645 g/ml
Molarity: 7.69 M (based on density)
pKa: approximately
Anti-CRMP1 (C2868)
(2000).
4. Deo, R.C., et al., EMBO J., 23, 9-22 (2004).
5. Yamashita, N., et al., J. Neurosci., 26, 13357-
13362 (2006).
6. Chang, C.C., et al., J. Natl. Cancer Inst., 96, 364-
375 (2004).
ER
Data Sheet - C8241
Reserved.
C8241dat Rev 03/22 NDH,PHC,GCY,MAM
2
Anti-CRMP-1 (N-terminal) (C4118)
(2000).
4. Deo, R.C., et al., EMBO J., 23, 9-22 (2004).
5. Yamashita, N., et al., J. Neurosci., 26, 13357-
13362 (2006).
6. Chang, C.C., et al., J. Natl. Cancer Inst., 96, 364-
375 (2004).
ER
Product Information Sheet - NA9604
W0263 1 x 375 ml
Wash Solution 2 W4639 1 x 75 ml
Elution Solution E7777 2 x 115 ml
Filter Plate F3555 4
Binding Plate B6686 4
Elution Plate (Corning
Costar cell culture plate)
CLS3790 4
AlumaSeal
User Guide 71015 - MPX TOP 7 STEC - AFNOR - EN
mEHEC® media.
For 375 g test portion, weigh into 1,500 mL pre-warmed (41.5 °C) mEHEC® media. Homogenize or mix
sample for 2 min. Incubate 25 g samples for 8 – 16 h at 41.5 °C. Incubate 375 g samples
User Guide 71015BC
media.
For 375 g test portion, weigh into 1,500 mL pre-warmed (41.5 °C) mEHEC® media. Homogenize or mix
sample for 2 min. Incubate 25 g samples for 8 – 16 h at 41.5 °C. Incubate 375 g samples for
User Guide - 60038BC
solution.
b. For 375 g samples, follow the directions in (c) above and also add 6.0 mL of 0.45% novobiocin solution.
c. Alternatively, follow the directions in (d) above and add 4 mL of
QC Range Sheet MCKP1-110K
Control 1 88 - 184 pg/mL IL-10 Control1 93- 192 pg/ml
Control 2 369 - 766 pg/mL Control 2 375 - 779 pg/ml
RABBIT ANTI-HUMAN INTEGRIN alpha4 POLYCLONAL ANTIBODY
. Fibronectin and its receptors. Ann. Rev. Biochem. 57: 375-413.
Hynes, R. O. (1987). Integrins: a family of cell surface receptors. Cell 48(4): 549-554.
Ruoslahti, E. and Pierschbacher, M. D. (1987
Product Information - FD500S
Gratath, K.A., Carbohydrate Res., 30, 375 (1973).
21. Arfors, K.E. and Hint, H., Microvasc. Res., 3, 440 (1971).
22. Arfors, K.E. and Rutili, G., Microvasc. Res., 4, 466 (1972).
23. Arfors, K.E. and Rutili
Product Information - FD150S
Gratath, K.A., Carbohydrate Res., 30, 375 (1973).
21. Arfors, K.E. and Hint, H., Microvasc. Res., 3, 440 (1971).
22. Arfors, K.E. and Rutili, G., Microvasc. Res., 4, 466 (1972).
23. Arfors, K.E. and Rutili
Product Information - FD40S
Gratath, K.A., Carbohydrate Res., 30, 375 (1973).
21. Arfors, K.E. and Hint, H., Microvasc. Res., 3, 440 (1971).
22. Arfors, K.E. and Rutili, G., Microvasc. Res., 4, 466 (1972).
23. Arfors, K.E. and Rutili
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