39382-08-6

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Performing a Separation with IgG Sepharose 6 Fast Flow

Perform a separation with IgG Sepharose 6 Fast Flow from Cytiva, an Affinity Chromatography product for purification of recombinant fusion proteins containing a protein A tail.

Enzymatic Activity of Glucose-6-Phosphatase [EC 3.1.3.9]

To measure glucose-6-phosphatase activity, the Taussky-Shorr method is used. This method is a spectrophotometric stop-rate determination assay that is measured at 660 nm.

Ni Sepharose 6 Fast Flow for Protein Purification

Ni Sepharose 6 Fast Flow purifies histidine-tagged proteins efficiently, offering high cross-linked agarose beads with Ni2+ ions.

Oligonucleotide Standard 6 Mix LC-UV Analysis

Chromolith® RP-18e columns optimize Oligo Standard 6 separation with varied flow rates and ion-pairing reagent evaluation.

Enzymatic Assay of Glucose-6-Phosphate Dehydrogenase (EC 1.1.1.49)

To measure glucose-6-phosphate dehydrogenase activity, beta-nicotinamide adenine dinucleotide phosphate is used in a spectrophotometric rate determination assay at 340 nm.

Dextran

Dextran polymer details: composed mainly of alpha-D-(1-6) linkages with varied branch lengths.

Performing a Separation or Removal of Albumin with HiTrap® Blue HP and Blue Sepharose 6 Fast Flow

This page shows how to separate or remove albumin by affinity chromatography using HiTrap Blue HP and Blue Sepharose 6 Fast Flow.

Enzymatic Assay of Peroxidase (EC 1.11.1.7) 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a Substrate

To standardize a procedure for the assay of Peroxidase using 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a substrate.

Removal of Albumin Using Blue Sepharose® Chromatography Media

Remove albumin from afﬁnity chromatography samples using HiTrap™ Blue HP or Blue Sepharose® 6 Fast Flow from Cytiva.

Determination of Hydrocortisone from Topical Cream Using Discovery DSC-Si SPE and Reversed-Phase HPLC-UV

Using the method described in this report, an average absolute recovery and RSD value of 99.86 ± 6.99% (n=6) was observed, to determine an average of 1.02% hydrocortisone in topical cream.

Enzymatic Assay of Alcohol Oxidase (EC 1.1.3.13)

To measure alcohol oxidase activity, this assay uses 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) and a continuous spectrophotometric rate determination at 405 nm.

Purification or Removal of DNA-Binding Proteins

This page shows how to purify or remove DNA-binding proteins with Heparin Sepharose High Performance, Heparin Sepharose 6 Fast Flow, Capto Heparin from Cytiva.

USDA FSIS STEC Guidance Implementation

Discover the expanded USDA FSIS verification testing for the 'Big 6' non-O157 STEC in beef products and explore accurate testing solutions and industry practices for enhanced food safety.

Preservation of Moisture-Sensitive Chemical Reagents

Preserve reagent quality of air- and moisture-sensitive reagents using nitrogen or argon in crown-cap bottles with a 6 mm diameter hole in the crown-cap and a PTFE-faced rubber liner.

Determination of Water Content in Phenol Using Karl Fischer Titration

Accurately measure the moisture content in Phenol (C6H5OH) through Karl Fischer titration, using both Volumetric and Coulometric methods.

Puriﬁcation of Histidine-Tagged Recombinant Proteins Using Ni Sepharose® High Performance

Ni Sepharose High Performance consists of highly cross-linked 6% agarose beads (34 µm) to which a chelating group has been immobilized and subsequently charged with Ni2+ ions.

Performing a Separation of DNA binding proteins with Cytiva Products Based on Heparin

This page shows how to use heparin in the separation of DNA binding proteins used in HiTrap Heparin HP, HiPrep 16/10 Heparin FF and Heparin Sepharose 6 Fast Flow products from Cytiva.

Purification of NAD+ and ATP-dependent Kinases

Affinity chromatography purification of enzymes using 5’ AMP Sepharose® 4B, HiTrap® Blue HP, and Blue Sepharose® 6 Fast Flow products.

Formulation and Delivery US 2024

Join us at the Formulation and Delivery US conference at booth #6 to learn about our integrated offering for all process steps in pharmaceutical and biopharmaceutical manufacturing which includes products that meet the highest quality and purity standards with extensive

Purification from Unclariﬁed Cell Lysate using HisTrap™ FF Crude

This page shows how to purify histidine-tagged recombinant proteins from unclariﬁed cell lysate using HisTrap FF crude from Cytiva.

Whole Transcriptome Amplification of RNA from Low Cell-Number Samples

The efficacy of amplification of small quantities of total RNA with the Complete Whole Transcriptome Amplification Kit (WTA2) was examined in this study.

TRC Library Citations

TRC 1.5 human and mouse shRNA libraries

Immunoprecipitation Techniques

This page describes immunoprecipitation (immunoaffinity or pull-down techniques).

Alkaline Phosphatase

We offer a broad range of alkaline phosphatase enzymes and substrates that are optimized for conjugation to antibodies and other proteins for your specific application needs.

Autophagy in Cancer Promotes Therapeutic Resistance

We presents an article on Autophagy in Cancer Promotes Therapeutic Resistance

Differentiation of Primary Human Osteoblasts

Cell culture protocol used to differentiate primary human osteoblasts into bone cells in 2-3 weeks.

Mesoporous Materials: Properties and Applications

Mesoporous Materials include a range of high surface area porous silicates with applications in gas adsorption, drug delivery, diagnostics and catalysis.

Flavors and Fragrances New Product Offerings

As a leading global supplier of new flavor and fragrance ingredients, we are dedicated to providing you with high-quality aroma chemicals comprising of certified food grade, IFRA, natural, kosher, and halal ingredients.

MISSION® Application Data

MISSION® Application Data. We are committed to providing you with helpful application notes and data for the MISSION® TRC shRNA libraries.

mRNA Health Conference

Join us at the 2022 mRNA Health Conference! Solutions for mRNA and Manufacturing and Formulation. Connect with our team and be sure to attend our technical presentations and poster sessions.

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