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MilliporeSigma Announces $65 Million Antibody-Drug Conjugate Manufacturing Expansion

MilliporeSigma today announced a $65 million expansion of its HPAPI and ADC manufacturing capabilities and capacity at its facility near Madison, Wisconsin.

Gold Nanorod Synthesis

Plasmonic nanoparticles have unique optical properties that can be tailored to suit a variety of applications in the biotechnology1–8 and electronics9–16 industries.

Chromolith® WidePore 300 HPLC Columns

Analytical Chromolith® HPLC columns for Bio applications, 300A mesopores: RP-18, RP-8, RP-4, Prot-A, Epoxy

GC Analysis of Aldehydes (PFBOA Derivatives) in Beer on SLB®-5ms after SPME using a 65 µm PDMS/DVB Fiber

Separation of null

Determination of vitamins B and preservatives

Learn to separate and quantify B vitamins and preservatives in capsules with HPLC-UV and Purospher® STAR RP-8 column. Validated for precision.

Famciclovir Tablets USP Monograph Method Using a Purospher™ STAR RP-8 endcapped HPLC Column and UV Detection

Following the new USP monograph for Famciclovir Tablets, Purospher™ STAR RP-8 endcapped HPLC columns can be used to monitor organic impurities in Famciclovir tablet formulations. Famciclovir is an antiviral drug indicated for the treatment of herpes zoster, herpes simplex virus

Determination of 11 Beta Agonist Residues in Pork by LC-MS

Using an Ascentis® Express F5 HPLC column, this study separates 11 β-agonists in pork by LC-MS/MS in under 8 minutes with excellent recovery and precision.

Determination of Eight Aminoglycosides in Pork Meat

LC/LC-MS method identifies 8 aminoglycosides in pork using Discovery® DSC-18 SPE and Ascentis® Express C18 UHPLC, per Chinese standards.

Detection of Signaling Effector-Complexes Downstream of BMP4 Using In Situ PLA, A Proximity Ligation Assay

BMPs are responsible for a wide range of developmental and biological effects. BMP receptors activate (phosphorylate) the Smad1/5/8 effectors, which then, form a complex with Smad4 and translocate to the nucleus where they function as transcription factors to initiate BMP

Column Packing and Preparation for Size Exclusion Chromatography

Peptide Stability and Potential Degradation Pathways

To prevent or minimize peptide degradation we recommend storing the peptide in lyophilized form at –20 °C or –80 °C. Once in solution, the peptide/protein should be stored frozen in individual aliquots to avoid freeze-thaw cycles. Also, prolonged exposure to

Thermal Transitions of Homopolymers: Glass Transition & Melting Point

Literature values for the glass transition temperature, (Tg), and melting temperature, (Tm), are given for the more common homopolymers.

Column Packing and Preparation for Affinity Chromatography of GST-tagged Proteins

This page describes efﬁcient column packing and preparation for affinity chromatography of GST-tagged proteins using Cytiva products.

Protein Determination using 2-D Quant Kit

2-D Quant Kit from Cytiva is designed to accurately determine protein concentrations in samples for electrophoresis.

HPLC Analysis of Protein Standards (1) on BIOshell™ A400 Protein C4

For use as a marker in SDS-PAGE; Albumin from chicken egg white, For use as a marker in SDS-PAGE; L-Lactic Dehydrogenase from rabbit muscle, Type XI, lyophilized powder, 600-1,200 units/mg protein

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

Nicewicz Photoredox Catalysts for Anti-Markovnikov Alkene Hydrofunctionalization

Explore Nicewicz Photoredox catalysts—photoexcitable acridinium salts for efficient, metal-free anti-Markovnikov hydrofunctionalizations.

Automated Solid Phase Microextraction (SPME) Sampling

Use the full range of Supelco® SPME fibers with your PAL autosampler system so you can sample smarter

Metal Borohydrides as Hydrogen Storage Materials

An article about metal borohydrides as hydrogen storage materials

Genomic DNA Purification using illustra™ GenomicPrep Mini Spin Kits

This page shows Genomic DNA purification using illustra™ genomicPrep Mini Spin Kits from Cytiva.

Isoelectric Focusing

Information on Isoelectric Focusing including what it is and how it is used. In order to ensure the high performance of analysis, isoelectric point (pI) standards are needed.

Standard Methods 6040D: GC Analysis of Geosmin and 2-MIB on SLB®-5ms after SPME using 50/30 µm DVB/Carboxen/PDMS Fiber

Separation of 2-Isopropyl-3-methoxypyrazine 20 ppt; 2-Isobutyl-3-methoxypyrazine; 2-Methylisoborneol; (±)-Geosmin 10 ppt

Sample Preparation for Chromatographic Purification

This page discusses various aspects of sample preparation for chromatographic purification.

Biomedical Implant Devices Fabricated from Low Young’s Modulus Titanium Alloys Demonstrating High Mechanical Biocompatibility

Biomedical implants are essentially foreign substances within the human body that must survive many years’ exposure to demanding mechanical and physiological conditions. Despite these challenges, metal implants have been widely used to substitute for or rebuild hard tissues such as

A Highly Sensitive IFN-γ ELISpot Assay to Quantify Cellular Immune Responses to Previous Viral Infection

This study presents an example of a typical ELISpot assay for quantifying the number of pre-existing, antigen-reactive T cells from peripheral blood mononuclear cell (PBMC) samples obtained from donors previously infected with cytomegalovirus (CMV).

Buchwald Phosphine Ligands

Genomic DNA Purification from Sample on FTA Elute

This page shows genomic DNA purification from sample on FTA elute from Cytiva.

Acid and Base Chart — Table of Acids & Bases

Acid and base chart lists the strength of acids and bases (strongest to weakest) in order. Simple to use laboratory reference chart for scientists, researchers and lab technicians.

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