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Product Information Sheet - MAK106
generate standards of 130, 65,
32.5, 16.3, and 8.1 pmole/well. 200 L of
2-propanol is used as the 0 (Blank) Standard.
Measure the fluorescence intensity (ex = 465/
em = 535 nm) of each
TGF-β Signaling: Dual Role in Tumor Suppression and Oncogenesis
PhosphoDetect™ Anti-
Smad1 (pSer463/465)
Rabbit pAb
566411 Polyclonal, protein A purified IgG. Recognizes the
~65 kDa Smad1 protein phosphorylated at
Ser463/465 in human and mouse. IB
50 g
Quality Control Range Sheet- PRCYT-108 and PRCYT-208
Control 1 82 - 171 pg/mL IL-23 Control 1 224 - 465 pg/mL
Control 2 373 - 775 pg/mL Control 2 989 - 2054 pg/mL
Data Sheet - P4247
J.S., J. Biol. Chem.
275, 29685-29693 (2000).
5. DeCoursey, T.E., et al., Nature, 307, 465-468
(1984).
6. Lewis, R.S., et al., Annu. Rev. Immunol., 13, 623-
653 (1995)
MCT/PHC 8/04
Sigma
Data Sheet - P4497
J.S., J. Biol. Chem.
275, 29685-29693 (2000).
5. DeCoursey, T. E., et al., Nature, 307, 465-468
(1984).
6. Lewis, R. S., et al., Annu. Rev. Immunol., 13, 623-
653 (1995).
DXP,PHC 05/08-1
Sigma
Data Sheet - A5106
optimal working dilutions by titration.
References
1. Pollard, T.D., and Borisy, G.G., Cell, 112, 453-465
(2003).
2. Takenawa, T., and Miki, H., J. Cell Sci., 114, 1801-
1809 (2001).
3. Millard, T.H
TGF-b Signaling: Dual Role in Tumor Suppression and Oncogenesis
PhosphoDetect™ Anti-Smad1
(pSer463/465) Rabbit pAb
566411
Polyclonal, protein A purified IgG. Recognizes
the ~65 kDa Smad1 protein phosphorylated
at Ser463/465 in human and mouse. IB
50 g
PhosphoDetect
Data Sheet - A5231
working concen-
trations by titration.
References
1. Pollard, T.D., and Borisy, G.G., Cell, 112, 453-465
(2003).
2. Takenawa, T., and Miki, H., J. Cell Sci., 114, 1801-
1809 (2001).
3. Millard, T.H
Data Sheet - G7646
Appl. Environ.
Microbiol., 65, 465-471 (1999).
5. Methods of Enzymatic Analysis, vol. 2, Hans Ulrich
Bergmeyer, Ed., Academic Press, NY, 460-461,
929-943 (1974).
6. Kim, D-H, et al., Biol. Pharm
Data Sheet - M9317
in rabbit using a synthetic
peptide corresponding to the C-terminus of human
MeCP2 (amino acids 465-478) conjugated to KLH as
immunogen. The sequence is conserved in rat and
mouse. Anti-MeCP2 is affinity-purified
Data Sheet - K3643 - Lot 019K1588
testis-representing a third isoform for the catalytic
subunit of cAMP-dependent protein kinase. Molec.
Endocr., 4, 465-475 (1990).
2. Reinton, N. et al., The gene encoding the C gamma
catalytic subunit of cAMP-dependent
Data Sheet - K3643 - Lot 040M0739
testis-representing a third isoform for the catalytic
subunit of cAMP-dependent protein kinase. Molec.
Endocr., 4, 465-475 (1990).
2. Reinton, N. et al., The gene encoding the C gamma
catalytic subunit of cAMP-dependent
XP725 Antibody Number-Lot Number 071M4826
75 76
1.2 5 6 7 8 29 30 31 32 53 54 55 56 77 78 79 80
1.3 9 10 11 12 33 34 35 36 57 58 59 60 81 82 83 84
1.4 13 14 15 16 37 38
Data Sheet - K3643
testis-representing a third isoform for the catalytic
subunit of cAMP-dependent protein kinase. Molec.
Endocr., 4, 465-475 (1990).
2. Reinton, N. et al., The gene encoding the C gamma
catalytic subunit of cAMP-dependent
Data Sheet - K3643 - Lot 090M0852
testis-representing a third isoform for the catalytic
subunit of cAMP-dependent protein kinase. Molec.
Endocr., 4, 465-475 (1990).
2. Reinton, N. et al., The gene encoding the C gamma
catalytic subunit of cAMP-dependent
Data Sheet - K3643 - Lot 031M0736
testis-representing a third isoform for the catalytic
subunit of cAMP-dependent protein kinase. Molec.
Endocr., 4, 465-475 (1990).
2. Reinton, N. et al., The gene encoding the C gamma
catalytic subunit of cAMP-dependent
Product Information Sheet - 11835238001
in US 10 735 094 001
1 kg, Not available in US 10 735 108 001
Tween 20 50 ml, 5 x 10 ml 11 332 465 001
1 2 3
1 2 3
TB392 pCDF-1b Vector Map
2807
BsaXI 2 137 2458
BseYI 3 1829 2592 2727
BsgI 2 3081 3281
BsiEI 5 189 465 933 1420 2347
BsiHKAI 4 150 1037 2049 3150
BsmAI 6 1361 1432 2511 2898 3024
3429
BsmBI 1 2511
BsmFI 2 752 1513
Bsp1286I 5
User Manual-ECM554
measure cell invasiveness and select for highly invasive tumor cells,
Proc. Natl. Acad. Sci. USA 83, 465-469.
28. Liotta, L.A. (1984) Tumor invasion and metastasis: role of the basement membrane, Am.
Product Information Sheet-11718096001
custom fill 10 223 638 103
Tris hydrochloride 500 g 10 812 846 001
Tween 20 50 ml, 5 x 10 ml 11 332 465 001
Buffers in a Box, Premixed PBS Buffer, 10x 4 l 11 666 789 001
Buffers in a Box
Product Information Sheet-12039672910
-Star, ready-to-use by alkaline phosphatase leads to a light emission
at a maximum wavelength of 465 nm (Figure 3) which is recorded with an imaging device or on X-ray film or
Lumi-Film*.
– Exposure
BioFiles-Issue
16805 Atto 425-NHS ester 1 mg
09260 Atto 425-Streptavidin 1 mg
50712 Atto 465 1 mg
55607 Atto 465-maleimide 1 mg
53404 Atto 465-NHS ester 1 mg
41051 Atto 488 1 mg
41698 Atto 488-NHS ester 1 mg
16951
Instruction Manual: siIMPORTER™ siRNA and Plasmid DNA Transfection Reagent
gene silencing.”
Cell. 107:465-76l.
3. Cogoni, C., N. Romano, and G. Macino. 1994. “Suppression
of gene expression by homologous transgenes.” Antonie
Van Leeuwenhoek. 65:205-9.
4. Kennerdell, J.
Product Information Sheet - 11697471001
sheets, 10 x 15 cm 11 209 299 001
1 roll, 0.3 x 3 m 11 417 240 001
Tween 20 50 ml, 5 x 10 ml 11 332 465 001
1 2 3
1 2 3
Product Information Sheet - S0501
, R., Anal. Profiles of Drug Subs., K. Florey, ed. Academic Press, NY, 6, 487, 1977.
3. Frank, M.J., et al., J. Pharm. Sci., 65, 44, 1976.
4. Bult, A., et al., Anal. Profiles of Drug Subs., K. Florey
Evolving strategies for application specific validation of research use antibodies
. 2008 Sep;130(3):465-71.
5. Brumbaugh K et al. Overview of the generation,
validation, and application of phosphosite-specific
antibodies. Methods Mol Biol. 2011;717:3-43.
6. Egelhofer, T.A.
Product Information Sheet-11755633001
sheets, 10 x 15 cm 11 209 299 001
1 roll, 0.3 x 3 m 11 417 240 001
Tween 20 50 ml, 5 x 10 ml 11 332 465 001
Blocking Reagent 50 g 11 096 176 001
Anti-Digoxigenin-AP, Fab fragments
Product Information Sheet-11655884001
sheets, 10 x 15 cm 11 209 299 001
1 roll, 0.3 x 3 m 11 417 240 001
Tween 20 50 ml, 5 x 10 ml 11 332 465 001
Blocking Reagent 50 g 11 096 176 001
Anti-Digoxigenin-AP, Fab fragments
Product Information Sheet-11093657910
l 11 666 681 001
Sodium Dodecyl Sulfate (SDS) 1 kg 11 667 289 001
Tween 20 50 ml, 5 x 10 ml 11 332 465 001
High Pure Plasmid Isolation Kit 1 kit, 50 purifications 11 754 777 001
1 kit
QCM 24-Well Collagen-Based Cell Invasion Assay
measure cell invasiveness and select for highly invasive tumor cells, Proc.
Natl. Acad. Sci. USA 83, 465-469.
8. Liotta, L.A. (1984) Tumor invasion and metastasis: role of the basement
membrane, Am.
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