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Product Information Sheet - SRE0065
References
1. Good, N.E. et al., Hydrogen ion buffers for
biological research. Biochemistry, 5(2), 466-467
(1966).
2. Grady, J.K. et al., Radicals from "Good's" buffers.
Anal. Biochem., 173(1)
Data Sheet - P7457
Protein to a nitrocellulose membrane.
2. Block the membrane using a solution of 5% non-fat
dry milk in TBS at 37 °C for 1 hour.
3. Wash the membrane twice for 1-2 minutes each in
TBS
Product Information Sheet - SRE0065
References
1. Good, N.E. et al., Hydrogen ion buffers for
biological research.
Biochemistry, 5(2), 466-467 (1966).
2. Grady, J.K. et al., Radicals from "Good's"
buffers. Anal.
Biochem., 173(1
Data Sheet - I3157
.
3. Donahue, R., et al., Blood, 75, 2271 (1990).
4. Petit-Frere, C., et al., Cytokine, 3, 466 (1991).
5. Renauld, J., et al., J. Immunol., 144, 4235 (1990).
6. Chang, M.S., et al., Blood, 83, 3199
Quality Control Range Sheet- MTH17-113 and MTH17-213
14563 pg/mL Control 2 3495 – 7703 pg/mL
MIP3A Control 1 684 – 1420 pg/mL IL-10 Control 1 224 – 466 pg/mL IL-31 Control 1 562 – 1167 pg/mL
Control 2
Product Information Sheet - S9797
et al., Nat. Protoc., 7(12), 2159‐2170
(2012).
5. Huang, Y. et al., PLoS One, 5(1), e8888 (2010).
6. Liu, Y. et al., Nat. Biotechnol. 37(4), 424-429
(2019).
Notice
We provide information
Data Sheet - A4594
Opin. Cell Biol.,
11, 466-475 (1999).
3. Spang, A., et al., Proc. Natl. Acad. Sci. USA, 95,
11199-11204 (1998).
4. Bremser, M., et al., Cell, 96, 495-506 (1999).
5. Brown, H. A., et
Datasheet - P7457
Protein to a nitrocellulose membrane.
2. Block the membrane using a solution of 5%
non-fat dry milk in TBS at 37 °C for 1 hour.
3. Wash the membrane twice for 1-2 minutes
MOUSE ANTI-CHOLINE ACETYLTRANSFERASE (ChAT) MONOCLONAL ANTIBODY
(pH 8.1) for 5 minutes at 37°C. Transfer sections to TBS (pH 8.1) containing
pronase (1.2 µg/mL) for 1 1/2-2 minutes at 37°C, followed by several ice cold buffer washes for a total of 5
minutes. The
Cellular Senescence Assay Kit
Supplied
1. 1X PBS (Phosphate Buffered Saline)
2. 37°C incubator
3. Phase contrast or light microscope
4. Polypropylene tubes
5. Cells / Tissue samples
6. 70% glycerol (optional)
Product Information Sheet - 11585592001
the DNA.
• After cooling to +37°C the DNA/agarose mixture can be
used directly for labeling.
� • Mix High Prime thoroughly and add 4 �l to the denatured
DNA.
• Add 5 μl 20 μCi [α32P]dCTP,
• Mix
XP725 Antibody Number-Lot Number 071M4826
3 4 25 26 27 28 49 50 51 52 73 74 75 76
1.2 5 6 7 8 29 30 31 32 53 54 55 56 77 78 79 80
1.3 9 10 11 12 33 34 35 36 57 58
Product Information Sheet-05401020001
Research Grade (Thermolysin Medium)
10 mg (2 × 5 mg)
Cat. No. 05 401 135 001 Liberase TH Research Grade (Thermolysin High)
10 mg (2 × 5 mg)
Cat. No. 05 466 202 001 Liberase DL Research Grade
Product Information Sheet-libdh-ro
Research Grade (Thermolysin Medium)
10 mg (2 × 5 mg)
Cat. No. 05 401 135 001 Liberase TH Research Grade (Thermolysin High)
10 mg (2 × 5 mg)
Cat. No. 05 466 202 001 Liberase DL Research Grade
Product Information Sheet - LIBDLRO
Research Grade (Thermolysin Medium)
10 mg (2 × 5 mg)
Cat. No. 05 401 135 001 Liberase TH Research Grade (Thermolysin High)
10 mg (2 × 5 mg)
Cat. No. 05 466 202 001 Liberase DL Research Grade
TB046VM pET-16b Vector Map
Psp1406I 5 881 2249 3148 4942 5315
PstI 1 4961
PvuI 1 5086
PvuII 3 1819 1912 3414
RcaI 4 617 4543 5551 5656
RsaI 4 165 1366 3629 5196
SapI 1 3707
Sau96I 22
Sau3AI 37
ScaI 1 5196
ScrFI 24
SfaNI 24
SfcI 5 138
Data Sheet - D4943
et al., Int. J. Biochem. Cell Bio., 36,
408-421 (2004).
15. Wrenger, S., et al., FEBS. Lett., 466, 155-159
(2000).
16. Shibuya-Saruta, H., et al., Clin. Lab. Anal. 10,
435-440 (1996).
Product Information Sheet-libth-ro
Research Grade (Thermolysin Medium)
10 mg (2 × 5 mg)
Cat. No. 05 401 135 001 Liberase TH Research Grade (Thermolysin High)
10 mg (2 × 5 mg)
Cat. No. 05 466 202 001 Liberase DL Research Grade
Product Information Sheet-libtm-ro
Research Grade (Thermolysin Medium)
10 mg (2 × 5 mg)
Cat. No. 05 401 135 001 Liberase TH Research Grade (Thermolysin High)
10 mg (2 × 5 mg)
Cat. No. 05 466 202 001 Liberase DL Research Grade
User Manual-S7350
slide.
2. Incubate overnight at 37 °C.
Day 2:
3. Treat cells with chemical (For example, H2O2).
4. PBS wash (2X)
5. Methanol fix the cells for 5 min.
Product Information Sheet - S0501
29, 1991.
45. Sadeghi-Hashjin, G. et al., Br. J. Pharmacol., 118, 466, 1996.
46. Noack, E. and Feelisch, M., Basic Res. Cardiol., 86, 37, 1991.
47. Fiscus, R.R., Semin. Thromb. Hemost., Supplement, 14
Product Information Sheet - SE120084
and guidelines for
the provision of a clinical biochemistry service,
Ann. Clin. Biochem., 1988; 25:466-483.
17. Tietz, N.W. ed., Clinical Guide to Laboratory
Tests, 3rd Edition, W.B. Saunders, Co.
The Extract-N-Amp Blood Kit: Rapid DNA Extraction Coupled with PCR
genomic DNA preparation from fresh or
frozen serum for PCR amplification. BioTechniques, 29, 460-466 (2000).
5. Panaccio, M, et al., FoLT PCR: a simple PCR protocol for amplifying DNA
directly from whole
Extract-N-Amp Tissue PCR Kit: Rapid Genomic DNA Extraction from Tissue Coupled with PCR
and found to be accurate.
30
25
20
15
10
5
35
40
45
0
50
ng
D
N
A
Extract #
10 wk, 37 ˚C
5
RT-PCR Product Listing
.48 (2001).
5. Okayama, H. and Berg, P., Molec. Cell. Biol., 2, 161 (1982).
6. Berger, S.L., et al., Biochemistry, 22, 2365 (1985).
7. Leis, J.P., et al., Proc. Natl. Acad. Sci. USA, 70, 466 (1973).
8.
Product Information Sheet - 5401046001
Low)
05 401 160 001
05 466 202 001
10 mg (2 × 5 mg)
100 mg (2 × 50 mg)
Liberase DH
Research Grade
(Dispase® High)
05 401 054 001
05 401 089 001
Product Information Sheet - SE120049
and guidelines for the provision of a
clinical biochemistry service. Ann. Clin. Biochem.,
1988; 25:466-483.
17. Tietz, N.W., ed., Clinical Guide to Laboratory Tests,
3rd Edition, W.B. Saunders, Co.
Phosphotyrosine HTRF® Assay
Buffer 15µL 10 µL 5 µL - 25µL 25µL
6. A total 25 µL reaction mix per well. Gently tap the plate to mix the solutions, cover with
one plate sealer. Incubate for
Product Information Sheet-cdp-ro
-distilled water.
Wash for 2 × 15 minutes at +37°C in 0.2 M NaOH containing 0.1% SDS* to remove the DIG-labeled probe.
Rinse thoroughly 5 minutes in 2x SSC*.
Phosphotyrosine HTRF® Assay
441 Biotin-TK3 5mM MgCl2
IGF-1R (h) 14-465 Biotin-TK2 5mM MgCl2, 1mM MnCl2
Insulin R (h) 14-466 Biotin-TK2 5mM MgCl2, 1mM MnCl2
Lck (h) 14-442 Biotin-TK3 5mM MgCl2, 1mM MnCl2
Lyn (h) 14-510
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