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476004-81-6
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Showing 1-30 of 720 results for "476004-81-6" within Site Content
Performing a Separation with IgG Sepharose 6 Fast Flow
Perform a separation with IgG Sepharose 6 Fast Flow from Cytiva, an Affinity Chromatography product for purification of recombinant fusion proteins containing a protein A tail.
Enzymatic Activity of Glucose-6-Phosphatase [EC 3.1.3.9]
To measure glucose-6-phosphatase activity, the Taussky-Shorr method is used. This method is a spectrophotometric stop-rate determination assay that is measured at 660 nm.
Ni Sepharose 6 Fast Flow for Protein Purification
Ni Sepharose 6 Fast Flow purifies histidine-tagged proteins efficiently, offering high cross-linked agarose beads with Ni2+ ions.
Oligonucleotide Standard 6 Mix LC-UV Analysis
Chromolith® RP-18e columns optimize Oligo Standard 6 separation with varied flow rates and ion-pairing reagent evaluation.
Enzymatic Assay of Glucose-6-Phosphate Dehydrogenase (EC 1.1.1.49)
To measure glucose-6-phosphate dehydrogenase activity, beta-nicotinamide adenine dinucleotide phosphate is used in a spectrophotometric rate determination assay at 340 nm.
Dextran
Dextran polymer details: composed mainly of alpha-D-(1-6) linkages with varied branch lengths.
Performing a Separation or Removal of Albumin with HiTrap® Blue HP and Blue Sepharose 6 Fast Flow
This page shows how to separate or remove albumin by affinity chromatography using HiTrap Blue HP and Blue Sepharose 6 Fast Flow.
Enzymatic Assay of Peroxidase (EC 1.11.1.7) 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a Substrate
To standardize a procedure for the assay of Peroxidase using 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a substrate.
Removal of Albumin Using Blue Sepharose® Chromatography Media
Remove albumin from affinity chromatography samples using HiTrap™ Blue HP or Blue Sepharose® 6 Fast Flow from Cytiva.
Enzymatic Assay of Alcohol Oxidase (EC 1.1.3.13)
To measure alcohol oxidase activity, this assay uses 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) and a continuous spectrophotometric rate determination at 405 nm.
Determination of Hydrocortisone from Topical Cream Using Discovery DSC-Si SPE and Reversed-Phase HPLC-UV
Using the method described in this report, an average absolute recovery and RSD value of 99.86 ± 6.99% (n=6) was observed, to determine an average of 1.02% hydrocortisone in topical cream.
Purification or Removal of DNA-Binding Proteins
This page shows how to purify or remove DNA-binding proteins with Heparin Sepharose High Performance, Heparin Sepharose 6 Fast Flow, Capto Heparin from Cytiva.
USDA FSIS STEC Guidance Implementation
Discover the expanded USDA FSIS verification testing for the 'Big 6' non-O157 STEC in beef products and explore accurate testing solutions and industry practices for enhanced food safety.
Determination of Water Content in Phenol Using Karl Fischer Titration
Accurately measure the moisture content in Phenol (C6H5OH) through Karl Fischer titration, using both Volumetric and Coulometric methods.
Purification of Histidine-Tagged Recombinant Proteins Using Ni Sepharose® High Performance
Ni Sepharose High Performance consists of highly cross-linked 6% agarose beads (34 µm) to which a chelating group has been immobilized and subsequently charged with Ni2+ ions.
Preservation of Moisture-Sensitive Chemical Reagents
Preserve reagent quality of air- and moisture-sensitive reagents using nitrogen or argon in crown-cap bottles with a 6 mm diameter hole in the crown-cap and a PTFE-faced rubber liner.
Performing a Separation of DNA binding proteins with Cytiva Products Based on Heparin
This page shows how to use heparin in the separation of DNA binding proteins used in HiTrap Heparin HP, HiPrep 16/10 Heparin FF and Heparin Sepharose 6 Fast Flow products from Cytiva.
Purification of NAD+ and ATP-dependent Kinases
Affinity chromatography purification of enzymes using 5’ AMP Sepharose® 4B, HiTrap® Blue HP, and Blue Sepharose® 6 Fast Flow products.
Formulation and Delivery US 2024
Join us at the Formulation and Delivery US conference at booth #6 to learn about our integrated offering for all process steps in pharmaceutical and biopharmaceutical manufacturing which includes products that meet the highest quality and purity standards with extensive
Dietary Antioxidants
Antioxidants protect biological systems from oxidative damage produced by oxygen-containing free radicals and from redoxactive transition metal ions such as iron, copper, and cadmium.
Thermal Transitions of Homopolymers: Glass Transition & Melting Point
Literature values for the glass transition temperature, (Tg), and melting temperature, (Tm), are given for the more common homopolymers.
Lung Cancer Organoid Biobank
Explore our cryopreserved human lung organoid biobank from NSCLC tumor biopsies for lung cancer, SARS-CoV2, COPD and pulmonary disease research. Lung organoid 3D cell culture protocol available.
Determination of Tanshinone IIA and key ginsenosides
Method development for HPLC-UV quantification of tanshinone IIA and key ginsenosides in Guanxin Danshen Diwan as per the Chinese Pharmacopoeia draft.
Events
Upcoming events at Merck KGaA, Darmstadt, Germany... We are a vibrant company that's driving innovation all over the world. Join us to learn more about scheduled events hosted or attented by Merck KGaA, Darmstadt, Germany.
Performing a Purification of NADP+-dependent dehydrogenases and other enzymes with affinity for NADP+ Using 2’5’ ADP Sepharose 4B and Red Sepharose CL-6B
This page shows how to purify NADP+-dependent dehydrogenases and other enzymes with affinity for NADP+ by affinity chromatography using 2’5’ ADP Sepharose 4B and Red Sepharose CL-6B from Cytiva.
Virus Concentration by Ultrafiltration
Ultrafiltration using Amicon® Ultra centrifugal devices for concentrating virus in preparation of high titer virus stocks and isolation of virus from samples.
Performing a Separation with HiTrap® Protein G HP
Purify monoclonal or polyclonal IgG from serum, cell culture supernatant or ascitic fluid using the HiTrap Protein G HP from Cytiva, an affinity-exclusion chromatography product containing Sepharose-immobilized Protein G.
Workflow for the Analysis of Polysorbate 80 in Erbitux® Formulation
A complete reversed phase HPLC-ELSD workflow, including sample preparation for the analysis of Polysorbate 80 in a monoclonal antibody formulation.
In Vitro Differentiation of Human PBMC and CD34+ Derived Monocytes into Mature CD83+/CD14- Dendritic Cells
Generate mature human dendritic immune cells from PBMCs or CD34+ stem cell derived monocytes in 7 days in defined and serum-free dendritic cell culture media. Explore over 350 PromoCell products.
General Instructions for Affinity Purification Using HiTrap® Columns
General instructions for affinity purification using HiTrap columns from Cytiva.
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