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53319
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Showing 1-30 of 197 results for "53319" within Papers
CRC Handbook of Microbiology, 4, 1-1 (1974)
Gene, 529(1), 181-185 (2013-08-21)
To describe at molecular level a family with pulverulent congenital cataract associated with a CRYGC gene mutation. One family with several affected members with pulverulent congenital cataract and 230 healthy controls were examined. Genomic DNA from leukocytes was isolated to
Acta physiologica Scandinavica. Supplementum, 492, 9-18 (1980-01-01)
An enzymic flux of O2- and H2O2 caused strand breaks in the supercoiled covalently closed circular Col El plasmid. Subnanomolar levels of superoxide levels of superoxide dismutase or of catalase prevented this attack on DNA, signifying that both O2- and
The Journal of biological chemistry, 288(20), 14310-14319 (2013-04-02)
NADH:ubiquinone oxidoreductase (complex I) pumps protons across the membrane using downhill redox energy. The Escherichia coli complex I consists of 13 different subunits named NuoA-N coded by the nuo operon. Due to the low abundance of the protein and some
Current opinion in organ transplantation, 19(2), 100-107 (2014-02-21)
To update the reader on the recent literature in liver, kidney, pancreas, and intestine static cold preservation, and to identify which solutions are most advantageous for each organ. The comparison of randomized trials of histidine-tryptophan-ketoglutarate (HTK), Celsior, and University of
Amino acid spacing in isotachophoresi; on polyacrylamide gel: a critical evaluation.
Analytical biochemistry, 94(1), 202-210 (1979-04-01)
Molecular pharmaceutics, 12(6), 1784-1797 (2015-04-17)
During synthesis, purification, and especially storage, antibody-drug conjugates (ADCs) may be exposed to various types of light. Several of the drugs commonly conjugated to antibodies contain photosensitive functional groups. Exposure to light could generate an excited state of the drug
Toxicology letters, 235(2), 84-95 (2015-03-25)
Sunscreen users have been increased, since excessive sun exposure increased the risk of skin diseases. Benzophenone (BP) and its derivatives are commonly used in sunscreens as UV blocker. Its photosafety is concern for human health. Our study showed the role
Journal of neurochemistry, 68(4), 1335-1344 (1997-04-01)
N-Acetyl-L-histidine (NAH) and N-acetyl-L-aspartic acid (NAA) are major constituents of vertebrate brain and eye with distinct phylogenetic distributions. They are characterized by high tissue concentrations, high tissue/extracellular fluid gradients, and a continuous regulated efflux into the extracellular fluid. As a
Related (βα)8-barrel proteins in histidine and tryptophan biosynthesis: a paradigm to study enzyme evolution.
Chembiochem : a European journal of chemical biology, 12(10), 1487-1494 (2011-06-10)
Biochimica et biophysica acta, 1838(7), 1760-1768 (2014-03-04)
The conformational dynamics of the histidine ABC transporter HisQMP2 from Salmonella enterica serovar Typhimurium, reconstituted into liposomes, is studied by site-directed spin labeling and double electron-electron resonance spectroscopy in the absence of nucleotides, in the ATP-bound, and in the post-hydrolysis
Proceedings of the National Academy of Sciences of the United States of America, 111(17), E1705-E1712 (2014-04-16)
The histidine imidazole side chain plays a critical role in protein function and stability. Its importance for catalysis is underscored by the fact that histidines are localized to active sites in ∼ 50% of all enzymes. NMR spectroscopy has become
Dalton transactions (Cambridge, England : 2003), 43(31), 11988-11999 (2014-06-28)
Ferrocene-conjugated copper(ii) complexes [Cu(Fc-aa)(aip)](ClO4) () and [Cu(Fc-aa)(pyip)](ClO4) () of l-amino acid reduced Schiff bases (Fc-aa), 2-(9-anthryl)-1H-imidazo[4,5-f][1,10]phenanthroline (aip) and 2-(1-pyrenyl)-1H-imidazo[4,5-f][1,10]phenanthroline (pyip), where Fc-aa is ferrocenylmethyl-l-tyrosine (Fc-Tyr in , ), ferrocenylmethyl-l-tryptophan (Fc-Trp in , ) and ferrocenylmethyl-l-methionine (Fc-Met in , ), were
Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry, 20(6), 989-1004 (2015-07-19)
This work is focused on the study of DNA binding and cleavage properties of 2'-deoxyadenosines modified with ester/amide of histidine (his(6)dA ester, his(6)dA amide) and their copper(II) complexes. To determine the coordination mode of the complex species potentiometric and spectroscopic
Prikladnaia biokhimiia i mikrobiologiia, 49(2), 149-154 (2013-06-26)
Strain MG 1655+hisGr hisL'-Delta, purR, which produces histidine with a weight yield of approximately 12% from glucose, was constructed through directed chromosomal modifications of the laboratory Escherichia coli strain MG 1655+, which has a known genome sequence. A feedback-resistant ATP-phosphoribosyl
The Journal of biological chemistry, 289(49), 34214-34228 (2014-10-25)
The extracellular protein HbpS from Streptomyces reticuli interacts with iron ions and heme. It also acts in concert with the two-component sensing system SenS-SenR in response to oxidative stress. Sequence comparisons suggested that the protein may bind a cobalamin. UV-visible
Nature, 505(7483), 427-431 (2013-12-24)
Acyl carrier protein (ACP) transports the growing fatty acid chain between enzymatic domains of fatty acid synthase (FAS) during biosynthesis. Because FAS enzymes operate on ACP-bound acyl groups, ACP must stabilize and transport the growing lipid chain. ACPs have a
Toxicology letters, 239(3), 182-193 (2015-10-07)
Solar UV radiation is main factor of photocarcinogenesis, photoageing, and phototoxicity; thus, protection from UV radiation is major concern. Sunscreens containing UV filters are suggested as sun safe practices, but safety of UV filters remains in controversies. Benzophenone-1 (BP1) is
International journal of pharmaceutics, 492(1-2), 127-136 (2015-07-19)
To evaluate the oxidative stability of proteins, a model protein, etanercept, was exposed to oxidative stress conditions using hydrogen peroxide. Various amino acids were also evaluated on their antioxidant effect. Transition temperature (Tm), secondary structural content, hydrodynamic size, and aggregation
PloS one, 10(8), e0135174-e0135174 (2015-08-14)
The budding yeast Saccharomyces cerevisiae has recently been described as an emerging opportunistic fungal pathogen. Fungal cell wall mannoproteins have been demonstrated to be involved in adhesion to inert surfaces and might be engaged in virulence. In this study, we
Bioorganic & medicinal chemistry letters, 18(15), 4303-4307 (2008-07-17)
An activation study of the human carbonic anhydrase (hCA, EC 4.2.1.1) isoforms hCA III (cytosolic) and IV (membrane-associated) with a series of natural and non-natural amino acids and aromatic/heterocyclic amines is reported. hCA III was efficiently activated by d-His, serotonin
The Analyst, 140(24), 8157-8164 (2015-11-17)
A novel, highly sensitive and selective dual-readout (colorimetric and fluorometric) sensor based on fluorescent carbon dots (CDs) and unmodified gold nanoparticles (AuNPs) for the detection of thiocyanate (SCN(-)) was proposed. Amino-functionalized CDs could be readily adsorbed onto the surface of
The FEBS journal, 280(23), 6184-6195 (2013-10-12)
Sulfite salts are widely used as antibrowning agents in food processing. Nevertheless, the exact mechanism by which sulfite prevents enzymatic browning has remained unknown. Here, we show that sodium hydrogen sulfite (NaHSO3) irreversibly blocks the active site of tyrosinase from
Methods (San Diego, Calif.), 54(1), 31-38 (2010-12-29)
Many recombinant proteins carry an oligohistidine (His(X))-tag that allows their purification by immobilized metal affinity chromatography (IMAC). This tag can be exploited for the site-specific attachment of chromophores and fluorophores, using the same metal ion-nitrilotriacetic acid (NTA) coordination chemistry that
Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry, 13(4), 481-498 (2008-04-18)
Early investigation of the electron paramagnetic resonance spectra of bis-histidine-coordinated membrane-bound ferriheme proteins led to the description of a spectral signal that had only one resolved feature. These became known as "highly anisotropic low-spin" or "large g(max)" ferriheme centers. Extensive
Biochemistry, 52(13), 2371-2380 (2013-03-21)
The pH dependence of the β-galactoside binding activity of human galectin-1 (hGal-1) was investigated by fluorescence spectroscopy using lactose as a ligand. The obtained binding constant Kb was 2.94 ± 0.10 mM(-1) at pH 7.5. The Kb value decreased at
Biochimica et biophysica acta, 1840(1), 434-442 (2013-10-15)
Nα-Acetylhistidine (NAH) is present in very high concentrations exclusively in the brain and lens of ectothermic vertebrates, including ray-finned fishes, amphibians and reptiles, and not in those of endothermic birds and mammals. Although NAH is known to be synthesized from
Current topics in medicinal chemistry, 13(22), 2866-2884 (2013-10-12)
Worldwide, tuberculosis is the leading cause of morbidity and mortality due to a single bacterial pathogen, Mycobacterium tuberculosis (Mtb). The increasing prevalence of this disease, the emergence of multi-, extensively, and totally drug-resistant strains, complicated by co-infection with the human
Biochemistry, 53(13), 2112-2125 (2014-03-15)
Hemophores from Pseudomonas aeruginosa (HasAp), Serratia marcescens (HasAsm), and Yersinia pestis (HasAyp) bind hemin between two loops. One of the loops harbors conserved axial ligand Tyr75 (Y75 loop) in all three structures, whereas the second loop (H32 loop) contains axial
Biotechnology letters, 33(6), 1075-1084 (2011-02-15)
Immobilized metal affinity chromatography (IMAC) of proteins containing poly-histidine fusion tags is an efficient research tool for purifying recombinant proteins from crude cellular feedstocks at laboratory scale. Nevertheless, to achieve successful purification of large amounts of the target protein for
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