53351-U

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Ascentis® Express Phenyl Hexyl Columns for U/HPLC

Improve your reversed phase selectivity for polar aromatics and heterocyclic compounds with the Ascentis® Express Phenyl-Hexyl U/HPLC column.

Prestige Antibody® Immunofluorescence

Immunofluorescence Protocol details Prestige Antibodies' subcellular localization studies in A-431, U-2 OS, and U-251MG cell lines.

Improving Glycoform Coverage for Mucin Prep

Human Protein Atlas uses A-431, U-251 MG, and U-205 cell lines with Prestige Antibodies for organelle mapping.

BIOshell™ Glycan U/HPLC Columns

The BIOshell™ Glycan column is ideal for the separation and quantitation of isolated glycans using the HILIC method for accurate glycoprotein analysis.

Newly Developed Porous Graphitic Carbon in U/HPLC

Polar graphitic carbon enables UHPLC separation of highly polar pesticides, herbicides, and Vitamin D metabolites, advancing liquid chromatography capabilities.

Ascentis® Express C8 U/HPLC Columns

When a C18 doesn′t give the desired separation, or your sample contains compounds that are known to be difficult to retain or resolve on a C18, consider changing to an Ascentis® Express C8 column.

Ascentis ® Express HILIC U/HPLC Columns

For separation of polar compounds including polar neutrals, polar acids, and polar and non-polar basic amines use our Ascentis® Express HILIC column.

Ascentis® Express U/HPLC Column Selection

The Ascentis® Express Selection Guide flowchart helps guide users in the selection of a phase based on the particular compound type or separation challenge.

Supel™ Carbon LC U/HPLC Columns

Supel™ Carbon LC U/HPLC columns facilitate high-temperature and high-pressure separation of polar or charged compounds.

BIOshell™ A400 Protein C4 U/HPLC Columns

BIOshell™ A400 Protein C4 columns contain 3.4 μm particles with 400 Å pores, which are derivatized with dimethylbutyl silane and exhaustively endcapped for optimum protein recovery.

Ascentis® Express F5 (Pentafluorophenyl) U/HPLC Columns

Enhanced selectivity for stereoisomers and closely related compounds with alternate selectivity from C18 phases for basic, acidic, or neutral compounds

The Importance of Filtration in Optimizing (U)HPLC and LC-MS Performance

Learn how filter selection and sample and mobile phase filtration impact HPLC back pressure and column lifetime.

Ascentis^® Express C18 U/HPLC Columns

Ascentis® Express C18 is the first choice when starting a new separation method.

Leverage the full potential of Pharmacopeia regulated U/HPLC method adaption to increase lab effectiveness

Learn strategies to improve productivity and reduce costs in your pharmaceutical analyses with U/HPLC method adaptation.

Determination of Glyphosate, Glufosinate, and AMPA in tea

A precise U/HPLC-MS/MS method with QuEChERS extraction for the analysis of glyphosate, glufosinate, and AMPA in green tea.

FastStart™ Taq DNA Polymerase, 5 U/μL Protocol & Troubleshooting

The choice of the PCR enzyme in combination with an appropriate buffer can profoundly affect PCR outcome.

Simplified LC-MS/MS for Herbicides in Cereals

Glyphosate analysis: LC-MS/MS method with Supel™ Carbon LC U/HPLC column for stability and retention.

Faster Protein LC (FP2LC)

Larger porous shell particles in BIOshell™ columns improve efficiency in U/HPLC analysis of peptides and proteins.

Ascentis^® Express Peptide ES-C18 U/HPLC Columns

Choose Ascentis® Express Peptide ES-C18 U/HPLC Columns based on Fused-Core® technology for fast and efficient separation of high-molecular weight compounds, such as peptides and small proteins. With a 2.7 µm particle size and rigorous testing, these columns offer reliable results

3D Neurosphere Culture Media

3D neurosphere culture protocol to expand human U-87 MG glioblastoma cells in serum-free/xeno-free stem cell culture media. Explore over 350 PromoCell products on Sigma.com.

SupelMIP Molecularly Imprinted Polymer SPE Cartridges

SupelMIP SPE is based on molecularly imprinted polymer (MIP) technology. Each SupelMIP phase offers tailor-made selectivity for the extraction of trace analytes in complex matrixes.

HPLC Discovery HS C18

HPLC DiscoveryBIO HS C18 products. Available in 3µm, 5µm and 10µm particle sizes.

HPLC Columns for Antibody and Large Protein Analysis

BIOshell™ IgG 1000 Å C4 columns are highly suited for the reversed phase separation of high molecular weight compounds such as monoclonal antibodies with molecular weight of 150 kDa.

DiscoveryBIO Wide Pore C18

DiscoveryBIO Wide Pore C18 products offered by Sigma-Aldrich.

Discovery BIO Wide Pore C5

Discovery BIO Wide Pore C5 products offered.

HybridSPE-Phospholipid Technology

This Sigma-Aldrich article discusses how the HybridSPE-Phospholipid Technology works and how the phospholipids are removed.

Automated Solid Phase Microextraction (SPME) Sampling

Use the full range of Supelco® SPME fibers with your PAL autosampler system so you can sample smarter

Metabolites – A Serious Challenge for LC/MS Separations and Usual Detection Techniques

We offer the tools for the analysis of the metabolites; including certified reference standards, enzymes, substrates, and chromatographic products.

Nucleic Acid Modifying Enzyme Selection Chart (Ultra Pure)

A helpful chart for selection of correct nucleic acid modifying enzyme.

ThermaStop™-RT PCR Additive

PCR additive interacts with reverse transcriptase at low temperatures to reduce priming problems that lead to nonspecific bands.

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