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Showing 1-30 of 33 results for "54793" within Papers
Noritaka Kato et al.
The journal of physical chemistry. B, 109(42), 19604-19612 (2006-07-21)
This study reports a homogeneous and competitive fluorescence quenching immunoassay based on gold nanoparticle/polyelectrolyte (Au(NP)/PE) coated latex particles prepared by the layer-by-layer (LbL) technique. First, the resonant energy transfer from a layer of fluorescent PEs to Au(NP) in LbL assembled
Yannis Dotsikas et al.
Journal of immunoassay & immunochemistry, 26(4), 285-293 (2005-09-13)
A simple method was developed for the rapid characterization of the covalent binding of haptens to proteins such as enzymes, bovine serum albumin (BSA), and other carrier-proteins and antibodies. In the present study, a commercially available fentanyl-BSA conjugate was characterized
M A Domin et al.
Rapid communications in mass spectrometry : RCM, 13(4), 222-226 (1999-03-31)
The ability to rapidly identify the taxonomic class of the wide variety of microorganisms involved in human and animal disease is becoming increasingly important, especially with the increasing development of resistance to the antibiotics which form the main defence against
Gunjan Pandey et al.
FEMS microbiology letters, 229(2), 231-236 (2003-12-19)
We have earlier reported a novel reductive pathway for o-nitrobenzoate (ONB) degradation (at 0.5 mM) in Arthrobacter protophormiae RKJ100, which proceeds via the formation of o-hydroxylaminobenzoate (HABA) and anthranilate (AA). During growth of this organism at 40 times higher concentration
Scott C Meyer et al.
Chemical biology & drug design, 68(1), 3-10 (2006-08-23)
Screening combinatorial libraries of conformationally constrained peptides against macromolecular targets is utilized in identifying novel drug leads and in developing new reagents for chemical biology. In methods such as phage-display selections, biotinylated macromolecular targets are often immobilized on avidin- and
Sotirios Zarogiannis et al.
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 23(5), 593-595 (2007-05-15)
This article reports on the development of a simple and cost-effective bioassay for the detection of biotin in urine and serum, based on the very selective binding of avidin and biotin. Avidin was allowed to react without isolating it from
Kai Qi et al.
Journal of the American Chemical Society, 126(21), 6599-6607 (2004-05-27)
Shell cross-linked nanoparticles (SCKs) presenting surface- and bioavailable biotin functional groups were synthesized via a mixed micelle methodology, whereby co-micellization of chain terminal biotinylated poly(acrylic acid)-b-poly(methyl acrylate) (PAA-b-PMA) and nonbiotinylated PAA-b-PMA were cross-linked in an intramicellar fashion within the shell
Juha A E Määttä et al.
Chembiochem : a European journal of chemical biology, 9(7), 1124-1135 (2008-04-03)
Chicken avidin is a key component used in a wide variety of biotechnological applications. Here we present a circularly permuted avidin (cpAvd4-->3) that lacks the loop between beta-strands 3 and 4. Importantly, the deletion of the loop has a positive
Thin-layer affinity chromatography in analysis of protein-ligand affinity.
M P Laitinen et al.
Analytical biochemistry, 243(2), 279-282 (1996-12-15)
Joan-Antoni Farrera et al.
Chemistry (Weinheim an der Bergstrasse, Germany), 14(7), 2277-2285 (2007-12-29)
The tautomeric equilibria of 2-(4'-hydroxyphenylazo)benzoic acid (HABA) and 2-(3',5'-dimethyl-4'-hydroxyphenylazo)benzoic acid (3',5'-dimethyl-HABA) have been studied by a combination of spectroscopic and computational methods. For neutral HABA in solvents of different polarity (toluene, chloroform, DMSO, DMF, butanol, and ethanol) the azo tautomer
D Leckband et al.
Biophysical journal, 69(3), 1162-1169 (1995-09-01)
The strength of receptor-mediated cell adhesion is directly controlled by the mechanism of cohesive failure between the cell surface and underlying substrate. Unbinding can occur either at the locus of the specific bond or within the bilayer, which results in
Cedric Przybylski et al.
Glycobiology, 20(2), 224-234 (2009-10-28)
Polysulfated carbohydrates such as heparin (HP) and heparan sulfate (HS) are not easily amenable to usual ultraviolet matrix-assisted laser desorption/ionization-mass spectrometry (UV-MALDI)-MS analysis due to the thermal lability of their O- and N-SO(3) moieties, and their poor ionization efficiency with
Nathan W Moore et al.
Langmuir : the ACS journal of surfaces and colloids, 24(4), 1212-1218 (2007-12-18)
According to classical thermodynamics, biological ligand-receptor bonds should have a median lifetime of about 2 ms, and nearly half should have lifetimes of nanoseconds to microseconds. As a result, it is clear that many "weak" bonds are indispensable for cellular
Susanna Repo et al.
Chemistry & biology, 13(10), 1029-1039 (2006-10-21)
The chicken genome encodes several biotin-binding proteins, including avidin and avidin-related protein 4 (AVR4). In addition to D-biotin, avidin binds an azo dye compound, 4-hydroxyazobenzene-2-carboxylic acid (HABA), but the HABA-binding properties of AVR4 are not yet known. Differential scanning calorimetry
Sajid Hussain et al.
Scientific reports, 4, 5232-5232 (2014-06-11)
Synaphic (ligand-directed) targeting of drugs is an important potential new approach to drug delivery, particularly in oncology. Considerable success with this approach has been achieved in the treatment of blood-borne cancers, but the advances with solid tumours have been modest.
K J Welham et al.
Rapid communications in mass spectrometry : RCM, 12(4), 176-180 (1998-03-11)
A variety of gram-positive and gram-negative intact bacterial cells have been analysed by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) and shown to provide fingerprint mass spectra with discrete peaks being observed over the mass range from 3 to 40 kDa.
Zhenying Zhang et al.
Rapid communications in mass spectrometry : RCM, 18(18), 2146-2154 (2004-08-19)
Six novel poly(phenylenevinylene) (PPV) derivatives carrying butoxy or myrtanyl groups, including poly(2-butoxy-m-phenylenevinylene) (Bu-MPV), poly(2,5-dibutoxy-p-phenylenevinylene-alter-p-phenylenevinylene) (Bu-PPPV), poly(2,5-dibutoxy-p-phenylenevinylene-alter-m-phenylenevinylene) (Bu-PMPV), poly(2-myrtanyl-m-phenylenevinylene) (Myr-MPV), poly(2,5-dimyrtanyl-p-phenylenevinylene-alter-p-phenylenevinylene) (Myr-PPPV), and poly(2,5-dimyrtanyl-p-phenylenevinylene-alter-m-phenylenevinylene) (Myr-PMPV), were characterized by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS). The repeat unit mass and the
Examination of complex oligosaccharides by matrix-assisted laser desorption/ionization mass spectrometry on time-of-flight and magnetic sector instruments
Harvey, D. J.; Rudd, P. M.; Bateman, R. H.; Bordoli, R. S.; Howes, K.; Hoyes, J. B.; Vickers, R. G.
Org. Mass Spectrom., 29, 753-765 (1994)
Ting Deng et al.
Biosensors & bioelectronics, 21(8), 1545-1552 (2005-08-09)
A quartz crystal microbalance (QCM) sensor was proposed for the detection of small molecule biotin based on the mixed self-assembled monolayer (SAM) of thiols on gold substrate and the bioaffinity difference between an analyte (biotin) and an analogue compound (HABA)
Mehmet Cinar et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 105, 80-87 (2013-01-09)
The optimized geometrical structure, vibrational and electronic transitions, chemical shifts and nonlinear optical properties of 2-(4-hydroxyphenylazo)benzoic acid (HABA) compound were presented in this study. The ground state geometrical structure and vibrational wavenumbers were carried out by using density functional (DFT/B3LYP)
Ferenc Zsila
Journal of molecular recognition : JMR, 24(6), 995-1006 (2011-11-01)
Promiscuous ligand binding by hen egg-white avidin has been demonstrated and studied by using circular dichroism (CD) spectroscopy complemented by molecular docking calculations. It has been shown that the biotin-binding pocket of avidin is able to accommodate a wide variety
Y Dai et al.
Carbohydrate research, 304(1), 1-9 (1997-12-24)
Sulfated oligosaccharides are an important class of compounds in the field of glycobiology. Mass spectrometric analysis of these molecules is challenging due to their readiness to dissociate in sample preparation and their tendency to fragment during ionization. Moreover, their presence
Kenneth Kam-Wing Lo et al.
Chemistry (Weinheim an der Bergstrasse, Germany), 12(5), 1500-1512 (2005-11-24)
Six luminescent cyclometalated iridium(III)-dipyridoquinoxaline and -dipyridophenazine complexes [Ir(ppy)2(N-N)](PF6) (Hppy = 2-phenylpyridine; N-N = dipyrido[3,2-f:2',3'-h]quinoxaline, dpq (1); 2-n-butylamidodipyrido[3,2-f:2',3'-h]quinoxaline, dpqa (2); 2-((2-biotinamido)ethyl)amidodipyrido[3,2-f:2',3'-h]quinoxaline, dpqB (3); dipyrido[3,2-a:2',3'-c]phenazine, dppz (4); benzo[i]dipyrido[3,2-a:2',3'-c]phenazine, dppn (5); 11-((2-biotinamido)ethyl)amidodipyrido[3,2-a:2',3'-c]phenazine, dppzB (6)) have been designed as luminescent intercalators for DNA and
H Hofstetter et al.
Analytical biochemistry, 284(2), 354-366 (2000-08-31)
We introduce a new nonradioactive, chromogenic label based on 4-hydroxyazobenzene-2-carboxylic acid (HABA), which is suitable for bioanalytical application, e.g., detection, localization, isolation, and purification. The HABA label is superior to other systems where it is difficult to separate labeled from
Wenhui Lv et al.
Journal of colloid and interface science, 356(1), 16-23 (2011-02-02)
A straightforward approach to the synthesis of "clickable" thermoresponsive core cross-linked (CCL) nanoparticles was developed. This approach was based on reversible addition-fragmentation chain transfer (RAFT) radical cross-linking polymerization of styrene and divinylbenzene with azide-functionalized poly(N-isopropylacrylamide) (PNIPAM-N(3)) as macro chain transfer
Kenneth Kam-Wing Lo et al.
Inorganic chemistry, 43(17), 5275-5282 (2004-08-18)
Two luminescent ruthenium(II) polypyridine complexes containing a biotin moiety [Ru(bpy)(2)(L1)](PF(6))(2) (1) and [Ru(bpy)(2)(L2)](PF(6))(2) (2) (bpy = 2,2'-bipyridine; L1 = 4-(N-((2-biotinamido)ethyl)amido)-4'-methyl-2,2'-bipyridine; L2 = 4-(N-((6-biotinamido)hexyl)amido)-4'-methyl-2,2'-bipyridine) have been synthesized and characterized, and their photophysical and electrochemical properties have been studied. Upon photoexcitation, complexes
Xiaoze Jiang et al.
Bioconjugate chemistry, 20(5), 994-1001 (2009-05-01)
Quantum dots (QDs) containing surface carboxylic groups have been successfully modified using biotinylated glycopolymer and carbohydrate/biotin reagents via EDC coupling. The biotinylated glycopolymer was synthesized in controlled dimension via the reversible addition-fragmentation chain transfer (RAFT) polymerization of the three monomers
Matrix-assisted laser desorption ionization mass spectrometry with 2-(4-hydroxyphenylazo) benzoic acid matrix.
Juhasz, Peter, Catherine E. Costello, and Klaus Biemann.
Journal of the American Society For Mass Spectrometry, 4 (5), 399-409 (1993)
Martina Bruna Violatto et al.
ACS nano, 13(4), 4410-4423 (2019-03-19)
Steroids are the standard therapy for autoimmune hepatitis (AIH) but the long-lasting administration is hampered by severe side effects. Methods to improve the tropism of the drug toward the liver are therefore required. Among them, conjugation to nanoparticles represents one
Characterization of Polymers by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry. End Group Determination and Molecular Weight Estimates in Poly(ethylene glycols)
Montaudo, Giorgio; Montaudo, Maurizio S.; Puglisi, Concetto; Samperi, Filippo
Macromolecules, 28, 4562-4569 (1995)
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