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60983
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Data Sheet - 60983
KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.
60983 Kovac's Reagent for indoles
(Indole Reagent according to Kovac)
Data Sheet - 44655
sterile culture tubes.
For further confirmation the indole test can be made with Kovac’s reagent (60983). Cover a colony with 10-20 µl
Kovac’s reagent. A change of color to red after 2-10 seconds shows
Data Sheet - 44657
indicates the presence of E. coli. In addition the
indole test can be made with Kovac’s reagent (Fluka 60983). Cover a colony with 10-20 µl Kovac’s reagent. A change of color to
red after 2-10 seconds shows
Data Sheet - 95273
check again for
fluorescence. For confirmation, the indole test can be made with Kovac’s reagent (60983). Cover a colony with 10-20
µl Kovac’s reagent. A change of color to red after 2-10 seconds shows
Data Sheet - 04313
coli. If no fluorescence occurs after 24 hours of incubation do not add Kovacs
reagent (Cat. No. 60983) for checking the indole reaction as this alcoholic reagent will destroy the
growth conditions for
Data Sheet - 31401
coli. If no fluorescence occurs after 24 hours of incubation do not add Kovacs reagent (Cat. No. 60983) for checking
the indole reaction as this alcoholic reagent will destroy the growth conditions for
Data Sheet - 22099
presence of E. coli. For
confirmation the indole test can be made with Kovac’s reagent (Cat. No. 60983). Cover a colony with 10-20 µl Kovac’s
reagent. A change of color to red after 2-10 seconds indicates
Data Sheet - 67309
peptone with a high tryptophane content.
Cat. No. 67309 contains Isoamylic alcohol and Cat. No. 60983 n-Butanol as solvent, they are more
stable than amyl alcohol described in the older formulations.
Data Sheet - 93657
amino-acid
tryptophan into indol and α-aminopropionic acid (3). Indol can be detected by Kovac’s reagent (60983,
67309) or Ehrlich’s reagent (39070, 39080) forming a red coloured complex that concentrates in a
Data Sheet - 81938
combination with the two Chromogens. To confirm Escherichia coIi,
add a drop ot Kovac's reagent (Cat. No 60983) on the dark-blue to violet colony. Formation of cherry-
red colour indicates the positive reaction
Data Sheet - 70194
of hydrolytic enzymes and as result indole
is formed. Indole can be detected with Kovacs Indole (60983) or Ehrlich’s Reagent (03891). Indole
combines with the aldehyde present in the reagent to form a
Data Sheet - 87959
combination with the two Chromogens. To
confirm Escherichia coIi, add a drop ot Kovac's reagent (Cat. No. 60983) on the dark-blue to violet colony. Formation
of cherry-red colour indicates the positive reaction
BROLACIN MUG Agar
check again for fluorescence.
In addition the indole test can be made with Kovac’s reagent (Cat. No. 60983). Cover a colony with 10-20 µl Kovac’s
reagent. A change of color to red after 2-10 seconds shows
Data Sheet - 17349
tube incubated at 44°C after 8 to 24 hours, perform indole test by adding Kovac‘s reagent (Cat. No.
60983). A positive indole test in a broth tube showing gas production at 44°C indicates the presence of
Data Sheet - 89823
technique can also be used. To confirm Escherichia coli, add a drop
of Kovac’s reagent (Cat. No. 60983) on the dark-blue to violet colony. Formation of cherry-red colour indicates the positive reaction
Data Sheet - 63014
presence
of E. coli. For confirmation the indole test can be made with Kovac’s reagent (Cat. No. 60983). Cover a
colony with 10-20 µl Kovac’s reagent. A change of color to red after 2-10 seconds indicates
Data Sheet - 61749
tube incubated at 44°C after 8 to 24 hours, perform indole test by adding Kovac‘s reagent (Cat. No.
60983). A positive indole test in a broth tube showing gas production at 44°C indicates the presence of
Data Sheet - 85927
technique can
also be used. To confirm Escherichia coli, add a drop of Kovac’s reagent (Cat. No. 60983) on the dark-
blue to violet colony. Formation of cherry-red colour indicates the positive reaction
Data Sheet - 16016
coli. If
there is no fluorescence after 24 hours of incubation do NOT add Kovacs reagent (Cat. No. 60983) to check the indole
reaction because this alcoholic reagent destroys the growth conditions for cultures
Data Sheet - 62634
coli. If there is no fluorescence after 24 hours of incubation do NOT add Kovacs reagent (Cat. No.
60983) to check the indole reaction because this alcoholic reagent destroys the growth conditions for
cultures
Data Sheet - 17165
indicates the presence of E. coli. In
addition the indole test can be made with Kovac’s reagent (60983). Cover a colony with 10-20 µl Kovac’s reagent. A
change of color to red after 2-10 seconds indicates
ID Membranes Flyer
acid. This reagent is for detecting the indole and
identify the indole-positive microorganisms.
60983 Kovac's Reagent for indoles
08714 Methyl Red Solution Some bacteria utilize glucose to form large