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User Guide-RAB0026
µL 200 µL 200 µL 200 µL 200 µL 200 µL 200 µL
Std 1 Std 2 Std 3 Std 4 Std 5 Std 6 Std 7 0 Std
Diluent
volume
Std +
720 µL
960 µL 300
Data Sheet - I9767
. Verma, I.M., and Stevenson, J., Proc. Natl. Acad.
Sci. USA, 94, 11758 (1997).
4. Regnier, C.H., et al., Cell, 90, 373 (1997).
5. DiDonato, J.A., et al., Nature, 388, 548 (1997).
6. Mercurio, F
GR 144053- Non-peptide antagonist of the platelet glycoprotein IIb/IIIa (GP IIb/IIIa) fibrinogen receptor
6989-6996 (1999).
117. Lazo, J.S., et al., Mol. Pharmacol., 61 , 720-728 (2002)
118. Myers, M.P., et al., Proc. Natl. Acad. Sci. USA, 94, 9052-9057 (1997).
119. Li, J., et al., Science, 275, 1943-1947 (
XP725 Antibody Number-Lot Number 071M4826
.5 689 690 691 692 713 714 715 716 737 738 739 740 761 762 763 764
8.6 693 694 695 696 717 718 719 720 741 742 743 744 765 766 767 768
Antibody number
Product Information Sheet-11855476001
001
720 U, 2 x 360 U, 190 reactions in a final volume
of 50 μl
11 681 842 001
3,600 U, 10 x 360 U, 950 reactions in a final volume
of 50 μl
11
Product Information Sheet - CS0270
ng/mL
Expected
ng/mL
Observed:
%Expected
Neat 2,880
1:2 1,340 1,440 93%
1:4 598 720 83%
1:8 296 360 82%
1:16 163 180 90%
1:32 70 90 78%
Product Information Sheet-elong-ro
Pre-Incubation 92 – 94 2 min 1
Denaturation
Annealing
Elongation
92 – 94
45 – 65(1)
68
10 sec
30 sec
45 sec – 30 min(2)
10
Denaturation
Annealing
Elongation
92 – 94
45
Product Information Sheet-ehifi-ro
Cycles
Pre-Incubation 94 2 min 1
Denaturation
Annealing
Elongation
94
45 – 65(1)
72(2) or 68
15 sec
30 sec
45 sec – 8 min(3)
10
Denaturation
Annealing
Elongation
94
45 – 68(1)
Excellent temperature homogeneity contributes to high PCR performance of the Mastercycler® nexus
93.5
94
94.5
95
95.5
96
660 665 670 675 680 685 690 695 700 705 710 715 720 725
Te
m
p
er
at
ur
Product Information Sheet-ehifidntp-ro
Cycles
Pre-Incubation 94 2 min 1
Denaturation
Annealing
Elongation
94
45 – 65(1)
72(2) or 68
15 sec
30 sec
45 sec – 8 min(3)
10
Denaturation
Annealing
Elongation
94
45 – 68(1)
Product Information Sheet - 11939823001
001
720 U, 2 x 360 U, 190 reactions in a final volume of 50 μl 11 681 842 001
3,600 U, 10 x 360 U, 950 reactions in a final volume of
50 μl
11 759 060 001
Taq
Product Information Sheet-3353621001
Reaction buffer, 10x conc. (Vial 5) 2.5
2 mM dATP (Vial 4) 2.5
Final Volume 24
– Mix the reaction mixture and spin down briefly.
Incubate for 3 minutes at +94°C.
– Chill on ice.
Protein Tyrosine Phosphatases- Potential Roles in Disease
6989-6996 (1999).
117. Lazo, J.S., et al., Mol. Pharmacol., 61 , 720-728 (2002)
118. Myers, M.P., et al., Proc. Natl. Acad. Sci. USA, 94, 9052-9057 (1997).
119. Li, J., et al., Science, 275, 1943-1947 (
Calbiochem Biologics 29.1
). Does not affect peptidylglutamyl- 5 mg
peptide hydrolyzing (PGPH)-like activity even at 100 µg/ml. Inhibits α-chymotrypsin, cathepsin L
and m-calpain (IC50 = 720 ng/ml, 190 ng/ml and 740 ng/ml, respectively
Biologics 29.1
). Does not affect peptidylglutamyl- 5 mg
peptide hydrolyzing (PGPH)-like activity even at 100 µg/ml. Inhibits α-chymotrypsin, cathepsin L
and m-calpain (IC50 = 720 ng/ml, 190 ng/ml and 740 ng/ml, respectively
Product Information Sheet-thificdnaro
required by the type of block cycler used.
Initial denaturation 94°C for 5 min
Standard PCR Profile Setup Temp. Time [s] Cycles
Denaturation 94°C 10 35
Annealing 50°C 20
Elongation 72°C 30
Final elongation
Shaping Epigenetics Discovery - Epigenetics Product Selection Brochure
3 4 5 6 M 2M 1
Thermocycling Conditions
94 °C for 2 minutes 1 cycle
98 °C for 1 s
5 cycles
74 °C for 1min/kb
98 °C
inNovations Newsletter #17
Denaturation 1 95°C, 2 min 95°C, 2 min 94°C, 2 min 94°C, 2 min
Amplification 25–40
95°C, 30 s
Primer (Tm – 5)°C,
30 s
72°C, 1 min per kbp
95°C, 30 s
Primer (Tm
XP725 Antibody List Lot 089K4791
NA M
696 Anti Cy3+Cy5 C0992 NA NA M
720 Anti Cy3+Cy5 C0992 NA NA M
730 b-Tubulin T5201 NA NA M y y y
744 Anti Cy3+Cy5 C0992 NA NA M
768 Anti Cy3+Cy5 C0992 NA NA M
94 N Cadherin-Ca in C2667C2667
107 CaM
XP725 Antibody List Lot 129K4831
NA M
696 Anti Cy3+Cy5 C0992 NA NA M
720 Anti Cy3+Cy5 C0992 NA NA M
730 b-Tubulin T5201 NA NA M y y y
744 Anti Cy3+Cy5 C0992 NA NA M
768 Anti Cy3+Cy5 C0992 NA NA M
94 N Cadherin-Ca in C2667C2667
107 CaM
XP725 Antibody List Lot 129K4830
NA M
696 Anti Cy3+Cy5 C0992 NA NA M
720 Anti Cy3+Cy5 C0992 NA NA M
730 b-Tubulin T5201 NA NA M y y y
744 Anti Cy3+Cy5 C0992 NA NA M
768 Anti Cy3+Cy5 C0992 NA NA M
94 N Cadherin-Ca in C2667C2667
107 CaM
New Products - Antibodies, Small Molecule, Inhibitors
and WHSC1 (IC50 > 50 µM). Inhibits the proliferation of
MV4-1, MOLM-13, KOPN-8 cells (IC50 = 170, 720, and 620 nM, respectively) and
suppresses the growth of MV4-11 xenografts in nude mice model (50 mg
Millipore's 2008 Sustainability Report
Region
(kWh in thousands)
744
2006 2007 2008
482
252
10
450 390
255
10
720
118,825
114,675
2006 2007 2008
79,000
37,350
2,475
72,375
Inhibitor Sourcebook 3rd Edition
Shown to be orally bioavailable with a serum half-life of 94 min in mice in vivo.
500 µg
SIRT1/2 Inhibitor IV, Cambinol 566323 [NSC-112546; 5-(2-hydroxy-naphthalen-1-ylmethyl)-6-
phenyl-2-thioxo-2
Protein Purification and Detection Tools
Livingston, D. M.
(1994) Cell 78, 161–172.
2. Roberts, S. G. E. and Green, M. (1994) Nature 371, 717–720.
3. Joneson, T., McDonough, M., Bar-Sag, D., and Van Aelst, L. (1996) Science 274, 1374–1376.
T7
Halal Certificate
HEXYL BUTYRATE NATURAL W256811 C37181 HC-22SIX782
719. HEXYL FORMATE W257001 C45646 HC-22SIJS80
720. HEXYL HEXANOATE 97%, FG W257206 A63493 HC-22SIS489
721. HEXYL ISOBUTYRATE 98%, FG W317209 A99332
Halal Certificate
HEXYL BUTYRATE NATURAL W256811 C37181 HC-24SIRA84
719. HEXYL FORMATE W257001 C45646 HC-24SIIR82
720. HEXYL HEXANOATE 97%, FG W257206 A63493 HC-24SIAP91
721. HEXYL ISOBUTYRATE 98%, FG W317209 A99332
TB055 pET System Manual
Rev. C 0611JN Page 47 of 63
Halling, S.M. and Smith, S. (1985) Bio/Technology 3, 715–720.
Harlow, E. and Lane, D. (1988) Antibodies, A Laboratory
XP725 Worksheet for Lot Number 071M4826
716 4 3 6 4 Histone H3 pSer28 H9908
717 4 3 6 5 Histone H3 pSer10 H0412
718 4 3 6 6 Histone H3 pSer10 H0412
719 4 3 6 7 Anti CY3/5 C0992
720 4 3 6 8 Anti CY3/5 C0992
721 4
List of Antibodies
n/d
717 Topoisomerase-I T8573 M y n/d n/d
718 TRAIL T3067 M y n/d n/d
719 TRAIL T9191 P y n/d n/d
720 Anti Cy3+Cy5 C0992 M NA NA NA
721 Transforming Growth Factor-β, pan T9429 P y n/d n/d
722 Transportin
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